Comparative Proteomic Analysis Reveals the Cross-Talk between the Responses Induced by H(2)O(2) and by Long-Term Rice Black-Streaked Dwarf Virus Infection in Rice

Hydrogen peroxide (H(2)O(2)) could be produced during the plant-virus compatible interaction. However, the cell responses regulated by the enhanced H(2)O(2) in virus infected plant are largely unknown. To make clear the influence of Rice black-streaked dwarf virus (RBSDV) infection on H(2)O(2) accumulation, we measured the content of H(2)O(2) and found the H(2)O(2) level was increased in rice seedlings inoculated with RBSDV. To reveal the responses initiated by the enhanced H(2)O(2) during plant-virus interaction, the present study investigated the global proteome changes of rice under long-term RBSDV infection. Approximately 1800 protein spots were detected on two-dimensional electrophoresis (2-DE) gels. Among them, 72 spots were found differently expressed, of which 69 spots were successfully identified by MALDI-TOF/TOF-MS. Furthermore, the differentially expressed proteins induced by RBSDV infection were compared to that induced by H(2)O(2). 19 proteins corresponding to 37 spots, which were differentially expressed under RBSDV infection, were observed differentially expressed under H(2)O(2) stress as well. These overlapping responsive proteins are mainly related to photosynthesis, redox homeostasis, metabolism, energy pathway, and cell wall modification. The increased H(2)O(2) in RBSDV infected plant may produce an oxidative stress, impair photosynthesis, disturb the metabolism, and eventually result in abnormal growth. The data provide a new understanding of the pivotal role of H(2)O(2) in rice-RBSDV compatible interaction.