DPPrimer – A Degenerate PCR Primer Design Tool

Designed degenerate primers unlike conventional primers are superior in matching and amplification of large number of genes, from related gene families. DPPrimer tool was designed to predict primers for PCR amplification of homologous gene from related or diverse plant species. The key features of t...

Descripción completa

Detalles Bibliográficos
Autores principales: Gahoi, Shachi, Arya, L, Anil, Rai, Marla, ss
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Biomedical Informatics 2013
Materias:
Software
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3842581/
https://www.ncbi.nlm.nih.gov/pubmed/24307773
http://dx.doi.org/10.6026/97320630009937
Descripción
Sumario:Designed degenerate primers unlike conventional primers are superior in matching and amplification of large number of genes, from related gene families. DPPrimer tool was designed to predict primers for PCR amplification of homologous gene from related or diverse plant species. The key features of this tool include platform independence and user friendliness in primer design. Embedded features such as search for functional domains, similarity score selection and phylogebetic tree further enhance the user friendliness of DPPrimer tool. Performance of DPPrimer tool was evaluated by successful PCR amplification of ADP-glucose phosphorylase genes from wheat, barley and rice. AVAILABILITY: DPPrimer is freely accessible at http://202.141.12.147/DGEN_tool/index.html

Ejemplares similares