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DPPrimer – A Degenerate PCR Primer Design Tool
Designed degenerate primers unlike conventional primers are superior in matching and amplification of large number of genes, from related gene families. DPPrimer tool was designed to predict primers for PCR amplification of homologous gene from related or diverse plant species. The key features of t...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Biomedical Informatics
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3842581/ https://www.ncbi.nlm.nih.gov/pubmed/24307773 http://dx.doi.org/10.6026/97320630009937 |
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author | Gahoi, Shachi Arya, L Anil, Rai Marla, ss |
author_facet | Gahoi, Shachi Arya, L Anil, Rai Marla, ss |
author_sort | Gahoi, Shachi |
collection | PubMed |
description | Designed degenerate primers unlike conventional primers are superior in matching and amplification of large number of genes, from related gene families. DPPrimer tool was designed to predict primers for PCR amplification of homologous gene from related or diverse plant species. The key features of this tool include platform independence and user friendliness in primer design. Embedded features such as search for functional domains, similarity score selection and phylogebetic tree further enhance the user friendliness of DPPrimer tool. Performance of DPPrimer tool was evaluated by successful PCR amplification of ADP-glucose phosphorylase genes from wheat, barley and rice. AVAILABILITY: DPPrimer is freely accessible at http://202.141.12.147/DGEN_tool/index.html |
format | Online Article Text |
id | pubmed-3842581 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Biomedical Informatics |
record_format | MEDLINE/PubMed |
spelling | pubmed-38425812013-12-04 DPPrimer – A Degenerate PCR Primer Design Tool Gahoi, Shachi Arya, L Anil, Rai Marla, ss Bioinformation Software Designed degenerate primers unlike conventional primers are superior in matching and amplification of large number of genes, from related gene families. DPPrimer tool was designed to predict primers for PCR amplification of homologous gene from related or diverse plant species. The key features of this tool include platform independence and user friendliness in primer design. Embedded features such as search for functional domains, similarity score selection and phylogebetic tree further enhance the user friendliness of DPPrimer tool. Performance of DPPrimer tool was evaluated by successful PCR amplification of ADP-glucose phosphorylase genes from wheat, barley and rice. AVAILABILITY: DPPrimer is freely accessible at http://202.141.12.147/DGEN_tool/index.html Biomedical Informatics 2013-11-11 /pmc/articles/PMC3842581/ /pubmed/24307773 http://dx.doi.org/10.6026/97320630009937 Text en © 2013 Biomedical Informatics This is an open-access article, which permits unrestricted use, distribution, and reproduction in any medium, for non-commercial purposes, provided the original author and source are credited. |
spellingShingle | Software Gahoi, Shachi Arya, L Anil, Rai Marla, ss DPPrimer – A Degenerate PCR Primer Design Tool |
title | DPPrimer – A Degenerate PCR Primer Design Tool |
title_full | DPPrimer – A Degenerate PCR Primer Design Tool |
title_fullStr | DPPrimer – A Degenerate PCR Primer Design Tool |
title_full_unstemmed | DPPrimer – A Degenerate PCR Primer Design Tool |
title_short | DPPrimer – A Degenerate PCR Primer Design Tool |
title_sort | dpprimer – a degenerate pcr primer design tool |
topic | Software |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3842581/ https://www.ncbi.nlm.nih.gov/pubmed/24307773 http://dx.doi.org/10.6026/97320630009937 |
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