DPPrimer – A Degenerate PCR Primer Design Tool

Designed degenerate primers unlike conventional primers are superior in matching and amplification of large number of genes, from related gene families. DPPrimer tool was designed to predict primers for PCR amplification of homologous gene from related or diverse plant species. The key features of t...

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Detalles Bibliográficos
Autores principales: Gahoi, Shachi, Arya, L, Anil, Rai, Marla, ss
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Biomedical Informatics 2013
Materias:
Software
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3842581/
https://www.ncbi.nlm.nih.gov/pubmed/24307773
http://dx.doi.org/10.6026/97320630009937
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author Gahoi, Shachi
Arya, L
Anil, Rai
Marla, ss
author_facet Gahoi, Shachi
Arya, L
Anil, Rai
Marla, ss
author_sort Gahoi, Shachi
collection PubMed
description Designed degenerate primers unlike conventional primers are superior in matching and amplification of large number of genes, from related gene families. DPPrimer tool was designed to predict primers for PCR amplification of homologous gene from related or diverse plant species. The key features of this tool include platform independence and user friendliness in primer design. Embedded features such as search for functional domains, similarity score selection and phylogebetic tree further enhance the user friendliness of DPPrimer tool. Performance of DPPrimer tool was evaluated by successful PCR amplification of ADP-glucose phosphorylase genes from wheat, barley and rice. AVAILABILITY: DPPrimer is freely accessible at http://202.141.12.147/DGEN_tool/index.html
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spelling pubmed-38425812013-12-04 DPPrimer – A Degenerate PCR Primer Design Tool Gahoi, Shachi Arya, L Anil, Rai Marla, ss Bioinformation Software Designed degenerate primers unlike conventional primers are superior in matching and amplification of large number of genes, from related gene families. DPPrimer tool was designed to predict primers for PCR amplification of homologous gene from related or diverse plant species. The key features of this tool include platform independence and user friendliness in primer design. Embedded features such as search for functional domains, similarity score selection and phylogebetic tree further enhance the user friendliness of DPPrimer tool. Performance of DPPrimer tool was evaluated by successful PCR amplification of ADP-glucose phosphorylase genes from wheat, barley and rice. AVAILABILITY: DPPrimer is freely accessible at http://202.141.12.147/DGEN_tool/index.html Biomedical Informatics 2013-11-11 /pmc/articles/PMC3842581/ /pubmed/24307773 http://dx.doi.org/10.6026/97320630009937 Text en © 2013 Biomedical Informatics This is an open-access article, which permits unrestricted use, distribution, and reproduction in any medium, for non-commercial purposes, provided the original author and source are credited.
spellingShingle Software
Gahoi, Shachi
Arya, L
Anil, Rai
Marla, ss
DPPrimer – A Degenerate PCR Primer Design Tool
title DPPrimer – A Degenerate PCR Primer Design Tool
title_full DPPrimer – A Degenerate PCR Primer Design Tool
title_fullStr DPPrimer – A Degenerate PCR Primer Design Tool
title_full_unstemmed DPPrimer – A Degenerate PCR Primer Design Tool
title_short DPPrimer – A Degenerate PCR Primer Design Tool
title_sort dpprimer – a degenerate pcr primer design tool
topic Software
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3842581/
https://www.ncbi.nlm.nih.gov/pubmed/24307773
http://dx.doi.org/10.6026/97320630009937
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