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Human Cytomegalovirus (HCMV) induces Human Endogenous Retrovirus (HERV) transcription

BACKGROUND: Emerging evidence suggests that human cytomegalovirus (HCMV) is highly prevalent in tumours of different origin. This virus is implied to have oncogenic and oncomodulatory functions, through its ability to control host gene expression. Human endogenous retroviruses (HERV) are also freque...

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Autores principales: Assinger, Alice, Yaiw, Koon-Chu, Göttesdorfer, Ingmar, Leib-Mösch, Christine, Söderberg-Nauclér, Cecilia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3842806/
https://www.ncbi.nlm.nih.gov/pubmed/24219971
http://dx.doi.org/10.1186/1742-4690-10-132
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author Assinger, Alice
Yaiw, Koon-Chu
Göttesdorfer, Ingmar
Leib-Mösch, Christine
Söderberg-Nauclér, Cecilia
author_facet Assinger, Alice
Yaiw, Koon-Chu
Göttesdorfer, Ingmar
Leib-Mösch, Christine
Söderberg-Nauclér, Cecilia
author_sort Assinger, Alice
collection PubMed
description BACKGROUND: Emerging evidence suggests that human cytomegalovirus (HCMV) is highly prevalent in tumours of different origin. This virus is implied to have oncogenic and oncomodulatory functions, through its ability to control host gene expression. Human endogenous retroviruses (HERV) are also frequently active in tumours of different origin, and are supposed to contribute as cofactors to cancer development. Due to the high prevalence of HCMV in several different tumours, and its ability to control host cell gene expression, we sought to define whether HCMV may affect HERV transcription. FINDINGS: Infection of 3 established cancer cell lines, 2 primary glioblastoma cells, endothelial cells from 3 donors and monocytes from 4 donors with HCMV (strains VR 1814 or TB40/F) induced reverse transcriptase (RT) activity in all cells tested, but the response varied between donors. Both, gammaretrovirus-related class I elements HERV-T, HERV-W, HERV-F and ERV-9, and betaretrovirus-related class II elements HML-2 - 4 and HML-7 - 8, as well as spuma-virus related class III elements of the HERV-L group were up-regulated in response to HCMV infection in GliNS1 cells. Up-regulation of HERV activity was more pronounced in cells harbouring active HCMV infection, but was also induced by UV-inactivated virus. The effect was only slightly affected by ganciclovir treatment and was not controlled by the IE72 or IE86 HCMV genes. CONCLUSIONS: Within this brief report we show that HCMV infection induces HERV transcriptional activity in different cell types.
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spelling pubmed-38428062013-11-29 Human Cytomegalovirus (HCMV) induces Human Endogenous Retrovirus (HERV) transcription Assinger, Alice Yaiw, Koon-Chu Göttesdorfer, Ingmar Leib-Mösch, Christine Söderberg-Nauclér, Cecilia Retrovirology Short Report BACKGROUND: Emerging evidence suggests that human cytomegalovirus (HCMV) is highly prevalent in tumours of different origin. This virus is implied to have oncogenic and oncomodulatory functions, through its ability to control host gene expression. Human endogenous retroviruses (HERV) are also frequently active in tumours of different origin, and are supposed to contribute as cofactors to cancer development. Due to the high prevalence of HCMV in several different tumours, and its ability to control host cell gene expression, we sought to define whether HCMV may affect HERV transcription. FINDINGS: Infection of 3 established cancer cell lines, 2 primary glioblastoma cells, endothelial cells from 3 donors and monocytes from 4 donors with HCMV (strains VR 1814 or TB40/F) induced reverse transcriptase (RT) activity in all cells tested, but the response varied between donors. Both, gammaretrovirus-related class I elements HERV-T, HERV-W, HERV-F and ERV-9, and betaretrovirus-related class II elements HML-2 - 4 and HML-7 - 8, as well as spuma-virus related class III elements of the HERV-L group were up-regulated in response to HCMV infection in GliNS1 cells. Up-regulation of HERV activity was more pronounced in cells harbouring active HCMV infection, but was also induced by UV-inactivated virus. The effect was only slightly affected by ganciclovir treatment and was not controlled by the IE72 or IE86 HCMV genes. CONCLUSIONS: Within this brief report we show that HCMV infection induces HERV transcriptional activity in different cell types. BioMed Central 2013-11-12 /pmc/articles/PMC3842806/ /pubmed/24219971 http://dx.doi.org/10.1186/1742-4690-10-132 Text en Copyright © 2013 Assinger et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Report
Assinger, Alice
Yaiw, Koon-Chu
Göttesdorfer, Ingmar
Leib-Mösch, Christine
Söderberg-Nauclér, Cecilia
Human Cytomegalovirus (HCMV) induces Human Endogenous Retrovirus (HERV) transcription
title Human Cytomegalovirus (HCMV) induces Human Endogenous Retrovirus (HERV) transcription
title_full Human Cytomegalovirus (HCMV) induces Human Endogenous Retrovirus (HERV) transcription
title_fullStr Human Cytomegalovirus (HCMV) induces Human Endogenous Retrovirus (HERV) transcription
title_full_unstemmed Human Cytomegalovirus (HCMV) induces Human Endogenous Retrovirus (HERV) transcription
title_short Human Cytomegalovirus (HCMV) induces Human Endogenous Retrovirus (HERV) transcription
title_sort human cytomegalovirus (hcmv) induces human endogenous retrovirus (herv) transcription
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3842806/
https://www.ncbi.nlm.nih.gov/pubmed/24219971
http://dx.doi.org/10.1186/1742-4690-10-132
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