An integrated strategy for efficient vector construction and multi-gene expression in Plasmodium falciparum

BACKGROUND: The construction of plasmid vectors for transgene expression in the malaria parasite, Plasmodium falciparum, presents major technical hurdles. Traditional molecular cloning by restriction and ligation often yields deletions and re-arrangements when assembling low-complexity (A + T)-rich...

Descripción completa

Detalles Bibliográficos
Autores principales: Wagner, Jeffrey C, Goldfless, Stephen J, Ganesan, Suresh M, Lee, Marcus CS, Fidock, David A, Niles, Jacquin C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3842810/
https://www.ncbi.nlm.nih.gov/pubmed/24160265
http://dx.doi.org/10.1186/1475-2875-12-373

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3842810/
https://www.ncbi.nlm.nih.gov/pubmed/24160265
http://dx.doi.org/10.1186/1475-2875-12-373

Ejemplares similares