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An integrated strategy for efficient vector construction and multi-gene expression in Plasmodium falciparum
BACKGROUND: The construction of plasmid vectors for transgene expression in the malaria parasite, Plasmodium falciparum, presents major technical hurdles. Traditional molecular cloning by restriction and ligation often yields deletions and re-arrangements when assembling low-complexity (A + T)-rich...
Autores principales: | Wagner, Jeffrey C, Goldfless, Stephen J, Ganesan, Suresh M, Lee, Marcus CS, Fidock, David A, Niles, Jacquin C |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3842810/ https://www.ncbi.nlm.nih.gov/pubmed/24160265 http://dx.doi.org/10.1186/1475-2875-12-373 |
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