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A Caenorhabditis elegans Locomotion Phenotype Caused by Transgenic Repeats of the hlh-17 Promoter Sequence
Transgene technology is one of the most heavily relied upon tools in modern biological research. Expression of an exogenous gene within cells, for research and therapeutic applications, nearly always includes promoters and other regulatory sequences. We found that repeats of a non-protein coding tra...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3842965/ https://www.ncbi.nlm.nih.gov/pubmed/24312354 http://dx.doi.org/10.1371/journal.pone.0081771 |
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author | Stout Jr, Randy F. Grubišić, Vladimir Parpura, Vladimir |
author_facet | Stout Jr, Randy F. Grubišić, Vladimir Parpura, Vladimir |
author_sort | Stout Jr, Randy F. |
collection | PubMed |
description | Transgene technology is one of the most heavily relied upon tools in modern biological research. Expression of an exogenous gene within cells, for research and therapeutic applications, nearly always includes promoters and other regulatory sequences. We found that repeats of a non-protein coding transgenic sequence produced profound changes to the behavior of the nematode Caenorhabditis elegans. These changes were produced by a glial promoter sequence but, unexpectedly, major deficits were observed specifically in backward locomotion, a neuron-driven behavior. We also present evidence that this behavioral phenotype is transpromoter copy number-dependent and manifests early in development and is maintained into adulthood of the worm. |
format | Online Article Text |
id | pubmed-3842965 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-38429652013-12-05 A Caenorhabditis elegans Locomotion Phenotype Caused by Transgenic Repeats of the hlh-17 Promoter Sequence Stout Jr, Randy F. Grubišić, Vladimir Parpura, Vladimir PLoS One Research Article Transgene technology is one of the most heavily relied upon tools in modern biological research. Expression of an exogenous gene within cells, for research and therapeutic applications, nearly always includes promoters and other regulatory sequences. We found that repeats of a non-protein coding transgenic sequence produced profound changes to the behavior of the nematode Caenorhabditis elegans. These changes were produced by a glial promoter sequence but, unexpectedly, major deficits were observed specifically in backward locomotion, a neuron-driven behavior. We also present evidence that this behavioral phenotype is transpromoter copy number-dependent and manifests early in development and is maintained into adulthood of the worm. Public Library of Science 2013-11-28 /pmc/articles/PMC3842965/ /pubmed/24312354 http://dx.doi.org/10.1371/journal.pone.0081771 Text en © 2013 Sout, Jr. et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Stout Jr, Randy F. Grubišić, Vladimir Parpura, Vladimir A Caenorhabditis elegans Locomotion Phenotype Caused by Transgenic Repeats of the hlh-17 Promoter Sequence |
title | A Caenorhabditis elegans Locomotion Phenotype Caused by Transgenic Repeats of the hlh-17 Promoter Sequence |
title_full | A Caenorhabditis elegans Locomotion Phenotype Caused by Transgenic Repeats of the hlh-17 Promoter Sequence |
title_fullStr | A Caenorhabditis elegans Locomotion Phenotype Caused by Transgenic Repeats of the hlh-17 Promoter Sequence |
title_full_unstemmed | A Caenorhabditis elegans Locomotion Phenotype Caused by Transgenic Repeats of the hlh-17 Promoter Sequence |
title_short | A Caenorhabditis elegans Locomotion Phenotype Caused by Transgenic Repeats of the hlh-17 Promoter Sequence |
title_sort | caenorhabditis elegans locomotion phenotype caused by transgenic repeats of the hlh-17 promoter sequence |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3842965/ https://www.ncbi.nlm.nih.gov/pubmed/24312354 http://dx.doi.org/10.1371/journal.pone.0081771 |
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