Heterologous Expression of Mycobacterial Esx Complexes in Escherichia coli for Structural Studies Is Facilitated by the Use of Maltose Binding Protein Fusions

The expression of heteroligomeric protein complexes for structural studies often requires a special coexpression strategy. The reason is that the solubility and proper folding of each subunit of the complex requires physical association with other subunits of the complex. The genomes of pathogenic m...

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Detalles Bibliográficos
Autores principales: Arbing, Mark A., Chan, Sum, Harris, Liam, Kuo, Emmeline, Zhou, Tina T., Ahn, Christine J., Nguyen, Lin, He, Qixin, Lu, Jamie, Menchavez, Phuong T., Shin, Annie, Holton, Thomas, Sawaya, Michael R., Cascio, Duilio, Eisenberg, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3843698/
https://www.ncbi.nlm.nih.gov/pubmed/24312350
http://dx.doi.org/10.1371/journal.pone.0081753

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3843698/
https://www.ncbi.nlm.nih.gov/pubmed/24312350
http://dx.doi.org/10.1371/journal.pone.0081753

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