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The Effect of Cationic Charge Density Change on Transfection Efficiency of Polyethylenimine

Objective(s): Polyethylenimine (PEI) is a potent non-viral gene delivery carrier. PEI surface charge plays a major role in its condensation ability which in turn is a necessary requirement for high transfection efficiency. As the PEI charge density is dependent on pH, the effect of pH changing PEI o...

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Autores principales: Rezvani Amin, Zohreh, Rahimizadeh, Mohammad, Eshghi, Hossein, Dehshahri, Ali, Ramezani, Mohammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3843858/
https://www.ncbi.nlm.nih.gov/pubmed/24298383
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author Rezvani Amin, Zohreh
Rahimizadeh, Mohammad
Eshghi, Hossein
Dehshahri, Ali
Ramezani, Mohammad
author_facet Rezvani Amin, Zohreh
Rahimizadeh, Mohammad
Eshghi, Hossein
Dehshahri, Ali
Ramezani, Mohammad
author_sort Rezvani Amin, Zohreh
collection PubMed
description Objective(s): Polyethylenimine (PEI) is a potent non-viral gene delivery carrier. PEI surface charge plays a major role in its condensation ability which in turn is a necessary requirement for high transfection efficiency. As the PEI charge density is dependent on pH, the effect of pH changing PEI on PEI condensation ability, transfection efficiency, and cytotoxicity was investigated. Materials and Methods: In order to compare the different values of pH, 25 kDa PEI solutions were prepared at the pH values of 5, 7 and 10, afterward complexed with two plasmids encoding reporter genes of luciferase and enhanced green fluorescence protein to evaluate the capability of polymers in plasmid delivery at two incubation time (4 versus 24 hr). Results: The condensation ability of PEI in acidic, neutral and basic environments was similar. At low pH value, the polyplex had negative surface charge whereas at a higher pH value, the surface charge increased and became positive. The higher transfection efficiency and lower cytotoxicity were achieved by the polyplexes prepared at the pH value of 5 with longer incubation time of 24 hr. Conclusion: The results showed the impact of pH value of PEI on biological and biophysical properties of polyplexes. Although the proton sponge effect can justify several properties of PEI, these results suggest that the proton sponge hypothesis may not be applicable for polymers with low buffering capacity at the pH values around 5.
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spelling pubmed-38438582013-12-02 The Effect of Cationic Charge Density Change on Transfection Efficiency of Polyethylenimine Rezvani Amin, Zohreh Rahimizadeh, Mohammad Eshghi, Hossein Dehshahri, Ali Ramezani, Mohammad Iran J Basic Med Sci Original Article Objective(s): Polyethylenimine (PEI) is a potent non-viral gene delivery carrier. PEI surface charge plays a major role in its condensation ability which in turn is a necessary requirement for high transfection efficiency. As the PEI charge density is dependent on pH, the effect of pH changing PEI on PEI condensation ability, transfection efficiency, and cytotoxicity was investigated. Materials and Methods: In order to compare the different values of pH, 25 kDa PEI solutions were prepared at the pH values of 5, 7 and 10, afterward complexed with two plasmids encoding reporter genes of luciferase and enhanced green fluorescence protein to evaluate the capability of polymers in plasmid delivery at two incubation time (4 versus 24 hr). Results: The condensation ability of PEI in acidic, neutral and basic environments was similar. At low pH value, the polyplex had negative surface charge whereas at a higher pH value, the surface charge increased and became positive. The higher transfection efficiency and lower cytotoxicity were achieved by the polyplexes prepared at the pH value of 5 with longer incubation time of 24 hr. Conclusion: The results showed the impact of pH value of PEI on biological and biophysical properties of polyplexes. Although the proton sponge effect can justify several properties of PEI, these results suggest that the proton sponge hypothesis may not be applicable for polymers with low buffering capacity at the pH values around 5. Mashhad University of Medical Sciences 2013-02 /pmc/articles/PMC3843858/ /pubmed/24298383 Text en © 2013: Iranian Journal of Basic Medical Sciences This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Rezvani Amin, Zohreh
Rahimizadeh, Mohammad
Eshghi, Hossein
Dehshahri, Ali
Ramezani, Mohammad
The Effect of Cationic Charge Density Change on Transfection Efficiency of Polyethylenimine
title The Effect of Cationic Charge Density Change on Transfection Efficiency of Polyethylenimine
title_full The Effect of Cationic Charge Density Change on Transfection Efficiency of Polyethylenimine
title_fullStr The Effect of Cationic Charge Density Change on Transfection Efficiency of Polyethylenimine
title_full_unstemmed The Effect of Cationic Charge Density Change on Transfection Efficiency of Polyethylenimine
title_short The Effect of Cationic Charge Density Change on Transfection Efficiency of Polyethylenimine
title_sort effect of cationic charge density change on transfection efficiency of polyethylenimine
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3843858/
https://www.ncbi.nlm.nih.gov/pubmed/24298383
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