Cargando…
Antidiabetic Effect of Oral Borapetol B Compound, Isolated from the Plant Tinospora crispa, by Stimulating Insulin Release
Aims. To evaluate the antidiabetic properties of borapetol B known as compound 1 (C1) isolated from Tinospora crispa in normoglycemic control Wistar (W) and spontaneously type 2 diabetic Goto-Kakizaki (GK) rats. Methods. The effect of C1 on blood glucose and plasma insulin was assessed by an oral gl...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3844206/ https://www.ncbi.nlm.nih.gov/pubmed/24319481 http://dx.doi.org/10.1155/2013/727602 |
_version_ | 1782293135114633216 |
---|---|
author | Lokman, Faradianna E. Gu, Harvest F. Wan Mohamud, Wan Nazaimoon Yusoff, Mashitah M. Chia, Keh Leong Östenson, Claes-Göran |
author_facet | Lokman, Faradianna E. Gu, Harvest F. Wan Mohamud, Wan Nazaimoon Yusoff, Mashitah M. Chia, Keh Leong Östenson, Claes-Göran |
author_sort | Lokman, Faradianna E. |
collection | PubMed |
description | Aims. To evaluate the antidiabetic properties of borapetol B known as compound 1 (C1) isolated from Tinospora crispa in normoglycemic control Wistar (W) and spontaneously type 2 diabetic Goto-Kakizaki (GK) rats. Methods. The effect of C1 on blood glucose and plasma insulin was assessed by an oral glucose tolerance test. The effect of C1 on insulin secretion was assessed by batch incubation and perifusion experiments using isolated pancreatic islets. Results. An acute oral administration of C1 improved blood glucose levels in treated versus placebo groups with areas under glucose curves 0–120 min being 72 ± 17 versus 344 ± 10 mmol/L (P < 0.001) and 492 ± 63 versus 862 ± 55 mmol/L (P < 0.01) in W and GK rats, respectively. Plasma insulin levels were increased by 2-fold in treated W and GK rats versus placebo group at 30 min (P < 0.05). C1 dose-dependently increased insulin secretion from W and GK isolated islets at 3.3 mM and 16.7 mM glucose. The perifusions of isolated islets indicated that C1 did not cause leakage of insulin by damaging islet beta cells (P < 0.001). Conclusion. This study provides evidence that borapetol B (C1) has antidiabetic properties mainly due to its stimulation of insulin release. |
format | Online Article Text |
id | pubmed-3844206 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-38442062013-12-08 Antidiabetic Effect of Oral Borapetol B Compound, Isolated from the Plant Tinospora crispa, by Stimulating Insulin Release Lokman, Faradianna E. Gu, Harvest F. Wan Mohamud, Wan Nazaimoon Yusoff, Mashitah M. Chia, Keh Leong Östenson, Claes-Göran Evid Based Complement Alternat Med Research Article Aims. To evaluate the antidiabetic properties of borapetol B known as compound 1 (C1) isolated from Tinospora crispa in normoglycemic control Wistar (W) and spontaneously type 2 diabetic Goto-Kakizaki (GK) rats. Methods. The effect of C1 on blood glucose and plasma insulin was assessed by an oral glucose tolerance test. The effect of C1 on insulin secretion was assessed by batch incubation and perifusion experiments using isolated pancreatic islets. Results. An acute oral administration of C1 improved blood glucose levels in treated versus placebo groups with areas under glucose curves 0–120 min being 72 ± 17 versus 344 ± 10 mmol/L (P < 0.001) and 492 ± 63 versus 862 ± 55 mmol/L (P < 0.01) in W and GK rats, respectively. Plasma insulin levels were increased by 2-fold in treated W and GK rats versus placebo group at 30 min (P < 0.05). C1 dose-dependently increased insulin secretion from W and GK isolated islets at 3.3 mM and 16.7 mM glucose. The perifusions of isolated islets indicated that C1 did not cause leakage of insulin by damaging islet beta cells (P < 0.001). Conclusion. This study provides evidence that borapetol B (C1) has antidiabetic properties mainly due to its stimulation of insulin release. Hindawi Publishing Corporation 2013 2013-11-10 /pmc/articles/PMC3844206/ /pubmed/24319481 http://dx.doi.org/10.1155/2013/727602 Text en Copyright © 2013 Faradianna E. Lokman et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Lokman, Faradianna E. Gu, Harvest F. Wan Mohamud, Wan Nazaimoon Yusoff, Mashitah M. Chia, Keh Leong Östenson, Claes-Göran Antidiabetic Effect of Oral Borapetol B Compound, Isolated from the Plant Tinospora crispa, by Stimulating Insulin Release |
title | Antidiabetic Effect of Oral Borapetol B Compound, Isolated from the Plant Tinospora crispa, by Stimulating Insulin Release |
title_full | Antidiabetic Effect of Oral Borapetol B Compound, Isolated from the Plant Tinospora crispa, by Stimulating Insulin Release |
title_fullStr | Antidiabetic Effect of Oral Borapetol B Compound, Isolated from the Plant Tinospora crispa, by Stimulating Insulin Release |
title_full_unstemmed | Antidiabetic Effect of Oral Borapetol B Compound, Isolated from the Plant Tinospora crispa, by Stimulating Insulin Release |
title_short | Antidiabetic Effect of Oral Borapetol B Compound, Isolated from the Plant Tinospora crispa, by Stimulating Insulin Release |
title_sort | antidiabetic effect of oral borapetol b compound, isolated from the plant tinospora crispa, by stimulating insulin release |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3844206/ https://www.ncbi.nlm.nih.gov/pubmed/24319481 http://dx.doi.org/10.1155/2013/727602 |
work_keys_str_mv | AT lokmanfaradiannae antidiabeticeffectoforalborapetolbcompoundisolatedfromtheplanttinosporacrispabystimulatinginsulinrelease AT guharvestf antidiabeticeffectoforalborapetolbcompoundisolatedfromtheplanttinosporacrispabystimulatinginsulinrelease AT wanmohamudwannazaimoon antidiabeticeffectoforalborapetolbcompoundisolatedfromtheplanttinosporacrispabystimulatinginsulinrelease AT yusoffmashitahm antidiabeticeffectoforalborapetolbcompoundisolatedfromtheplanttinosporacrispabystimulatinginsulinrelease AT chiakehleong antidiabeticeffectoforalborapetolbcompoundisolatedfromtheplanttinosporacrispabystimulatinginsulinrelease AT ostensonclaesgoran antidiabeticeffectoforalborapetolbcompoundisolatedfromtheplanttinosporacrispabystimulatinginsulinrelease |