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Islet-1 Immunoreactivity in the Developing Retina of Xenopus laevis
The LIM-homeodomain transcription factor Islet1 (Isl1) has been widely used as a marker of neuronal differentiation in the developing visual system of different classes of vertebrates, including mammals, birds, reptiles, and fish. In the present study, we analyzed the spatial and temporal distributi...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3844241/ https://www.ncbi.nlm.nih.gov/pubmed/24348185 http://dx.doi.org/10.1155/2013/740420 |
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author | Álvarez-Hernán, Guadalupe Bejarano-Escobar, Ruth Morona, Ruth González, Agustín Martín-Partido, Gervasio Francisco-Morcillo, Javier |
author_facet | Álvarez-Hernán, Guadalupe Bejarano-Escobar, Ruth Morona, Ruth González, Agustín Martín-Partido, Gervasio Francisco-Morcillo, Javier |
author_sort | Álvarez-Hernán, Guadalupe |
collection | PubMed |
description | The LIM-homeodomain transcription factor Islet1 (Isl1) has been widely used as a marker of neuronal differentiation in the developing visual system of different classes of vertebrates, including mammals, birds, reptiles, and fish. In the present study, we analyzed the spatial and temporal distribution of Isl1-immunoreactive cells during Xenopus laevis retinal development and its relation to the formation of the retinal layers, and in combination with different markers of cell differentiation. The earliest Isl1 expression appeared at St29-30 in the cell nuclei of sparse differentiating neuroblasts located in the vitreal surface of the undifferentiated retina. At St35-36, abundant Isl1-positive cells accumulated at the vitreal surface of the neuroepithelium. As development proceeded and through the postmetamorphic juveniles, Isl1 expression was identified in subpopulations of ganglion cells and in subsets of amacrine, bipolar, and horizontal cells. These data together suggest a possible role for Isl1 in the early differentiation and maintenance of different retinal cell types, and Isl1 can serve as a specific molecular marker for the study of retinal cell specification in X. laevis. |
format | Online Article Text |
id | pubmed-3844241 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-38442412013-12-12 Islet-1 Immunoreactivity in the Developing Retina of Xenopus laevis Álvarez-Hernán, Guadalupe Bejarano-Escobar, Ruth Morona, Ruth González, Agustín Martín-Partido, Gervasio Francisco-Morcillo, Javier ScientificWorldJournal Research Article The LIM-homeodomain transcription factor Islet1 (Isl1) has been widely used as a marker of neuronal differentiation in the developing visual system of different classes of vertebrates, including mammals, birds, reptiles, and fish. In the present study, we analyzed the spatial and temporal distribution of Isl1-immunoreactive cells during Xenopus laevis retinal development and its relation to the formation of the retinal layers, and in combination with different markers of cell differentiation. The earliest Isl1 expression appeared at St29-30 in the cell nuclei of sparse differentiating neuroblasts located in the vitreal surface of the undifferentiated retina. At St35-36, abundant Isl1-positive cells accumulated at the vitreal surface of the neuroepithelium. As development proceeded and through the postmetamorphic juveniles, Isl1 expression was identified in subpopulations of ganglion cells and in subsets of amacrine, bipolar, and horizontal cells. These data together suggest a possible role for Isl1 in the early differentiation and maintenance of different retinal cell types, and Isl1 can serve as a specific molecular marker for the study of retinal cell specification in X. laevis. Hindawi Publishing Corporation 2013-11-11 /pmc/articles/PMC3844241/ /pubmed/24348185 http://dx.doi.org/10.1155/2013/740420 Text en Copyright © 2013 Guadalupe Álvarez-Hernán et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Álvarez-Hernán, Guadalupe Bejarano-Escobar, Ruth Morona, Ruth González, Agustín Martín-Partido, Gervasio Francisco-Morcillo, Javier Islet-1 Immunoreactivity in the Developing Retina of Xenopus laevis |
title | Islet-1 Immunoreactivity in the Developing Retina of Xenopus laevis
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title_full | Islet-1 Immunoreactivity in the Developing Retina of Xenopus laevis
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title_fullStr | Islet-1 Immunoreactivity in the Developing Retina of Xenopus laevis
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title_full_unstemmed | Islet-1 Immunoreactivity in the Developing Retina of Xenopus laevis
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title_short | Islet-1 Immunoreactivity in the Developing Retina of Xenopus laevis
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title_sort | islet-1 immunoreactivity in the developing retina of xenopus laevis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3844241/ https://www.ncbi.nlm.nih.gov/pubmed/24348185 http://dx.doi.org/10.1155/2013/740420 |
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