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CD59 Underlines the Antiatherosclerotic Effects of C-Phycocyanin on Mice

The effects of C-phycocyanin (C-PC) on atherosclerosis and the regulatory effects of CD59 gene on anti-atherosclerotic roles of C-PC were investigated. Apolipoprotein E knockout (ApoE(−/−)) mice were randomly divided into four groups: control group, C-PC treatment group, CD59 transfection group and...

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Autores principales: Li, Bing, Chu, Xian-Ming, Xu, Ying-Jie, Yang, Fan, Lv, Cong-Yi, Nie, Shu-min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3844276/
https://www.ncbi.nlm.nih.gov/pubmed/24319687
http://dx.doi.org/10.1155/2013/729413
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author Li, Bing
Chu, Xian-Ming
Xu, Ying-Jie
Yang, Fan
Lv, Cong-Yi
Nie, Shu-min
author_facet Li, Bing
Chu, Xian-Ming
Xu, Ying-Jie
Yang, Fan
Lv, Cong-Yi
Nie, Shu-min
author_sort Li, Bing
collection PubMed
description The effects of C-phycocyanin (C-PC) on atherosclerosis and the regulatory effects of CD59 gene on anti-atherosclerotic roles of C-PC were investigated. Apolipoprotein E knockout (ApoE(−/−)) mice were randomly divided into four groups: control group, C-PC treatment group, CD59 transfection group and C-PC+CD59 synergy group. The mice were fed with high-fat-diet and treated with drug intervention at the same time. Results showed the atherosclerotic mouse model was successfully established. CD59 was over-expressed in blood and tissue cells. Single CD59 or C-PC could reduce blood lipid levels and promote the expression of anti-apoptotic Bcl-2 but inhibit pro-apoptotic Fas proteins in endothelial cells. The expression levels of cell cycle protein D1 (Cyclin D1) and mRNA levels of cyclin dependent protein kinase 4 (CDK4) in smooth muscle cells were restrained by CD59 and C-PC. CD59 or C-PC alone could inhibit the formation of atherosclerotic plaque by suppressing MMP-2 protein expression. In addition, C-PC could promote CD59 expression. So both CD59 and C-PC could inhibit the progress of atherosclerosis, and the anti-atherosclerotic effects of C-PC might be fulfilled by promoting CD59 expression, preventing smooth muscle cell proliferation and the apoptosis of endothelial cells, reducing blood fat levels, and at last inhibiting the development of atherosclerosis.
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spelling pubmed-38442762013-12-08 CD59 Underlines the Antiatherosclerotic Effects of C-Phycocyanin on Mice Li, Bing Chu, Xian-Ming Xu, Ying-Jie Yang, Fan Lv, Cong-Yi Nie, Shu-min Biomed Res Int Research Article The effects of C-phycocyanin (C-PC) on atherosclerosis and the regulatory effects of CD59 gene on anti-atherosclerotic roles of C-PC were investigated. Apolipoprotein E knockout (ApoE(−/−)) mice were randomly divided into four groups: control group, C-PC treatment group, CD59 transfection group and C-PC+CD59 synergy group. The mice were fed with high-fat-diet and treated with drug intervention at the same time. Results showed the atherosclerotic mouse model was successfully established. CD59 was over-expressed in blood and tissue cells. Single CD59 or C-PC could reduce blood lipid levels and promote the expression of anti-apoptotic Bcl-2 but inhibit pro-apoptotic Fas proteins in endothelial cells. The expression levels of cell cycle protein D1 (Cyclin D1) and mRNA levels of cyclin dependent protein kinase 4 (CDK4) in smooth muscle cells were restrained by CD59 and C-PC. CD59 or C-PC alone could inhibit the formation of atherosclerotic plaque by suppressing MMP-2 protein expression. In addition, C-PC could promote CD59 expression. So both CD59 and C-PC could inhibit the progress of atherosclerosis, and the anti-atherosclerotic effects of C-PC might be fulfilled by promoting CD59 expression, preventing smooth muscle cell proliferation and the apoptosis of endothelial cells, reducing blood fat levels, and at last inhibiting the development of atherosclerosis. Hindawi Publishing Corporation 2013 2013-11-12 /pmc/articles/PMC3844276/ /pubmed/24319687 http://dx.doi.org/10.1155/2013/729413 Text en Copyright © 2013 Bing Li et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Li, Bing
Chu, Xian-Ming
Xu, Ying-Jie
Yang, Fan
Lv, Cong-Yi
Nie, Shu-min
CD59 Underlines the Antiatherosclerotic Effects of C-Phycocyanin on Mice
title CD59 Underlines the Antiatherosclerotic Effects of C-Phycocyanin on Mice
title_full CD59 Underlines the Antiatherosclerotic Effects of C-Phycocyanin on Mice
title_fullStr CD59 Underlines the Antiatherosclerotic Effects of C-Phycocyanin on Mice
title_full_unstemmed CD59 Underlines the Antiatherosclerotic Effects of C-Phycocyanin on Mice
title_short CD59 Underlines the Antiatherosclerotic Effects of C-Phycocyanin on Mice
title_sort cd59 underlines the antiatherosclerotic effects of c-phycocyanin on mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3844276/
https://www.ncbi.nlm.nih.gov/pubmed/24319687
http://dx.doi.org/10.1155/2013/729413
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