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Profiling circulating microRNA expression in a mouse model of nerve allotransplantation

BACKGROUND: The lack of noninvasive biomarkers of rejection remains a challenge in the accurate monitoring of deeply buried nerve allografts and precludes optimization of therapeutic intervention. This study aimed to establish the expression profile of circulating microRNAs (miRNAs) during nerve all...

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Autores principales: Rau, Cheng-Shyuan, Yang, Johnson Chia-Shen, Wu, Shao-Chun, Chen, Yi-Chun, Lu, Tsu-Hsiang, Lin, Ming-Wei, Wu, Yi-Chan, Tzeng, Siou-Ling, Wu, Chia-Jung, Hsieh, Ching-Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3844622/
https://www.ncbi.nlm.nih.gov/pubmed/24011263
http://dx.doi.org/10.1186/1423-0127-20-64
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author Rau, Cheng-Shyuan
Yang, Johnson Chia-Shen
Wu, Shao-Chun
Chen, Yi-Chun
Lu, Tsu-Hsiang
Lin, Ming-Wei
Wu, Yi-Chan
Tzeng, Siou-Ling
Wu, Chia-Jung
Hsieh, Ching-Hua
author_facet Rau, Cheng-Shyuan
Yang, Johnson Chia-Shen
Wu, Shao-Chun
Chen, Yi-Chun
Lu, Tsu-Hsiang
Lin, Ming-Wei
Wu, Yi-Chan
Tzeng, Siou-Ling
Wu, Chia-Jung
Hsieh, Ching-Hua
author_sort Rau, Cheng-Shyuan
collection PubMed
description BACKGROUND: The lack of noninvasive biomarkers of rejection remains a challenge in the accurate monitoring of deeply buried nerve allografts and precludes optimization of therapeutic intervention. This study aimed to establish the expression profile of circulating microRNAs (miRNAs) during nerve allotransplantation with or without immunosuppression. RESULTS: Balb/c mice were randomized into 3 experimental groups, that is, (1) untreated isograft (Balb/c → Balb/c), (2) untreated allograft (C57BL/6 → Balb/c), and (3) allograft (C57BL/6 → Balb/c) with FK506 immunosuppression. A 1-cm Balb/c or C57BL/6 donor sciatic nerve graft was transplanted into sciatic nerve gaps created in recipient mice. At 1, 3, 7, 10, and 14 d after nerve transplantation, nerve grafts, whole blood, and sera were obtained for miRNA expression analysis with an miRNA array and subsequent validation with quantitative real-time PCR (qRT-PCR). Three circulating miRNAs (miR-320, miR-762, and miR-423-5p) were identified in the whole blood and serum of the mice receiving an allograft with FK506 immunosuppression, within 2 weeks after nerve allotransplantation. However, these 3 circulating miRNAs were not expressed in the nerve grafts. The expression of all these 3 upregulated circulating miRNAs significantly decreased at 2, 4, and 6 d after discontinuation of FK506 immunosuppression. In the nerve graft, miR-125-3b and miR-672 were significantly upregulated in the mice that received an allograft with FK506 only at 7 d after nerve allotransplantation. CONCLUSIONS: We identified the circulating miR-320, miR-762, and miR-423-5p as potential biomarkers for monitoring the immunosuppression status of the nerve allograft. However, further research is required to investigate the mechanism behind the dysregulation of these markers and to evaluate their prognostic value in nerve allotransplantation.
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spelling pubmed-38446222013-12-02 Profiling circulating microRNA expression in a mouse model of nerve allotransplantation Rau, Cheng-Shyuan Yang, Johnson Chia-Shen Wu, Shao-Chun Chen, Yi-Chun Lu, Tsu-Hsiang Lin, Ming-Wei Wu, Yi-Chan Tzeng, Siou-Ling Wu, Chia-Jung Hsieh, Ching-Hua J Biomed Sci Research BACKGROUND: The lack of noninvasive biomarkers of rejection remains a challenge in the accurate monitoring of deeply buried nerve allografts and precludes optimization of therapeutic intervention. This study aimed to establish the expression profile of circulating microRNAs (miRNAs) during nerve allotransplantation with or without immunosuppression. RESULTS: Balb/c mice were randomized into 3 experimental groups, that is, (1) untreated isograft (Balb/c → Balb/c), (2) untreated allograft (C57BL/6 → Balb/c), and (3) allograft (C57BL/6 → Balb/c) with FK506 immunosuppression. A 1-cm Balb/c or C57BL/6 donor sciatic nerve graft was transplanted into sciatic nerve gaps created in recipient mice. At 1, 3, 7, 10, and 14 d after nerve transplantation, nerve grafts, whole blood, and sera were obtained for miRNA expression analysis with an miRNA array and subsequent validation with quantitative real-time PCR (qRT-PCR). Three circulating miRNAs (miR-320, miR-762, and miR-423-5p) were identified in the whole blood and serum of the mice receiving an allograft with FK506 immunosuppression, within 2 weeks after nerve allotransplantation. However, these 3 circulating miRNAs were not expressed in the nerve grafts. The expression of all these 3 upregulated circulating miRNAs significantly decreased at 2, 4, and 6 d after discontinuation of FK506 immunosuppression. In the nerve graft, miR-125-3b and miR-672 were significantly upregulated in the mice that received an allograft with FK506 only at 7 d after nerve allotransplantation. CONCLUSIONS: We identified the circulating miR-320, miR-762, and miR-423-5p as potential biomarkers for monitoring the immunosuppression status of the nerve allograft. However, further research is required to investigate the mechanism behind the dysregulation of these markers and to evaluate their prognostic value in nerve allotransplantation. BioMed Central 2013-09-05 /pmc/articles/PMC3844622/ /pubmed/24011263 http://dx.doi.org/10.1186/1423-0127-20-64 Text en Copyright © 2013 Rau et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Rau, Cheng-Shyuan
Yang, Johnson Chia-Shen
Wu, Shao-Chun
Chen, Yi-Chun
Lu, Tsu-Hsiang
Lin, Ming-Wei
Wu, Yi-Chan
Tzeng, Siou-Ling
Wu, Chia-Jung
Hsieh, Ching-Hua
Profiling circulating microRNA expression in a mouse model of nerve allotransplantation
title Profiling circulating microRNA expression in a mouse model of nerve allotransplantation
title_full Profiling circulating microRNA expression in a mouse model of nerve allotransplantation
title_fullStr Profiling circulating microRNA expression in a mouse model of nerve allotransplantation
title_full_unstemmed Profiling circulating microRNA expression in a mouse model of nerve allotransplantation
title_short Profiling circulating microRNA expression in a mouse model of nerve allotransplantation
title_sort profiling circulating microrna expression in a mouse model of nerve allotransplantation
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3844622/
https://www.ncbi.nlm.nih.gov/pubmed/24011263
http://dx.doi.org/10.1186/1423-0127-20-64
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