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Quantitative analysis of the Brucella suis proteome reveals metabolic adaptation to long-term nutrient starvation

BACKGROUND: During the infection process, bacteria are confronted with various stress factors including nutrient starvation. In an in vitro model, adaptation strategies of nutrient-starved brucellae, which are facultative intracellular pathogens capable of long-term persistence, were determined. RES...

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Autores principales: Al Dahouk, Sascha, Jubier-Maurin, Véronique, Neubauer, Heinrich, Köhler, Stephan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3844638/
https://www.ncbi.nlm.nih.gov/pubmed/24007556
http://dx.doi.org/10.1186/1471-2180-13-199
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author Al Dahouk, Sascha
Jubier-Maurin, Véronique
Neubauer, Heinrich
Köhler, Stephan
author_facet Al Dahouk, Sascha
Jubier-Maurin, Véronique
Neubauer, Heinrich
Köhler, Stephan
author_sort Al Dahouk, Sascha
collection PubMed
description BACKGROUND: During the infection process, bacteria are confronted with various stress factors including nutrient starvation. In an in vitro model, adaptation strategies of nutrient-starved brucellae, which are facultative intracellular pathogens capable of long-term persistence, were determined. RESULTS: Long-term nutrient starvation in a medium devoid of carbon and nitrogen sources resulted in a rapid decline in viability of Brucella suis during the first three weeks, followed by stabilization of the number of viable bacteria for a period of at least three weeks thereafter. A 2D-Difference Gel Electrophoresis (DIGE) approach allowed the characterization of the bacterial proteome under these conditions. A total of 30 proteins showing altered concentrations in comparison with bacteria grown to early stationary phase in rich medium were identified. More than half of the 27 significantly regulated proteins were involved in bacterial metabolism with a marked reduction of the concentrations of enzymes participating in amino acid and nucleic acid biosynthesis. A total of 70% of the significantly regulated proteins showed an increased expression, including proteins involved in the adaptation to harsh conditions, in regulation, and in transport. CONCLUSIONS: The adaptive response of Brucella suis most likely contributes to the long-term survival of the pathogen under starvation conditions, and may play a key role in persistence.
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spelling pubmed-38446382013-12-02 Quantitative analysis of the Brucella suis proteome reveals metabolic adaptation to long-term nutrient starvation Al Dahouk, Sascha Jubier-Maurin, Véronique Neubauer, Heinrich Köhler, Stephan BMC Microbiol Research Article BACKGROUND: During the infection process, bacteria are confronted with various stress factors including nutrient starvation. In an in vitro model, adaptation strategies of nutrient-starved brucellae, which are facultative intracellular pathogens capable of long-term persistence, were determined. RESULTS: Long-term nutrient starvation in a medium devoid of carbon and nitrogen sources resulted in a rapid decline in viability of Brucella suis during the first three weeks, followed by stabilization of the number of viable bacteria for a period of at least three weeks thereafter. A 2D-Difference Gel Electrophoresis (DIGE) approach allowed the characterization of the bacterial proteome under these conditions. A total of 30 proteins showing altered concentrations in comparison with bacteria grown to early stationary phase in rich medium were identified. More than half of the 27 significantly regulated proteins were involved in bacterial metabolism with a marked reduction of the concentrations of enzymes participating in amino acid and nucleic acid biosynthesis. A total of 70% of the significantly regulated proteins showed an increased expression, including proteins involved in the adaptation to harsh conditions, in regulation, and in transport. CONCLUSIONS: The adaptive response of Brucella suis most likely contributes to the long-term survival of the pathogen under starvation conditions, and may play a key role in persistence. BioMed Central 2013-09-04 /pmc/articles/PMC3844638/ /pubmed/24007556 http://dx.doi.org/10.1186/1471-2180-13-199 Text en Copyright © 2013 Al Dahouk et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Al Dahouk, Sascha
Jubier-Maurin, Véronique
Neubauer, Heinrich
Köhler, Stephan
Quantitative analysis of the Brucella suis proteome reveals metabolic adaptation to long-term nutrient starvation
title Quantitative analysis of the Brucella suis proteome reveals metabolic adaptation to long-term nutrient starvation
title_full Quantitative analysis of the Brucella suis proteome reveals metabolic adaptation to long-term nutrient starvation
title_fullStr Quantitative analysis of the Brucella suis proteome reveals metabolic adaptation to long-term nutrient starvation
title_full_unstemmed Quantitative analysis of the Brucella suis proteome reveals metabolic adaptation to long-term nutrient starvation
title_short Quantitative analysis of the Brucella suis proteome reveals metabolic adaptation to long-term nutrient starvation
title_sort quantitative analysis of the brucella suis proteome reveals metabolic adaptation to long-term nutrient starvation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3844638/
https://www.ncbi.nlm.nih.gov/pubmed/24007556
http://dx.doi.org/10.1186/1471-2180-13-199
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