Cytotoxicity Evaluation of Proroot MTA, Root MTA and Portland Cement on Human Gingival Fibroblasts

INTRODUCTION: The aim of this study was to compare the cytotoxicity of ProRoot MTA (PMTA), Root MTA (RMTA) and Portland cement (PC) on human gingival fibroblasts (HGFs). MATERIALS AND METHODS: The extracts of the test materials were placed close to human gingival fibroblasts (HGFs) within 96-well pl...

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Autores principales: Sharifian, Mohammad Reza, Ghobadi, Mehrnoosh, Shokouhinejad, Noushin, Assadian, Hadi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Iranian Center for Endodontic Research 2007
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3844761/
https://www.ncbi.nlm.nih.gov/pubmed/24298288
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author Sharifian, Mohammad Reza
Ghobadi, Mehrnoosh
Shokouhinejad, Noushin
Assadian, Hadi
author_facet Sharifian, Mohammad Reza
Ghobadi, Mehrnoosh
Shokouhinejad, Noushin
Assadian, Hadi
author_sort Sharifian, Mohammad Reza
collection PubMed
description INTRODUCTION: The aim of this study was to compare the cytotoxicity of ProRoot MTA (PMTA), Root MTA (RMTA) and Portland cement (PC) on human gingival fibroblasts (HGFs). MATERIALS AND METHODS: The extracts of the test materials were placed close to human gingival fibroblasts (HGFs) within 96-well plates. Cellular viability was assessed using MTT assay in different intervals (freshly mixed, 4, 24, and 168 hours after mixing). The data were analyzed using the One-way ANOVA and Tukey’s test at 95% significance level. RESULTS: It was indicated that there was not a significant difference in cytotoxicity of test materials (p>0.05). In addition, there was not a statistically significant difference between different time intervals within each group (p>0.05). CONCLUSION: PMTA, RMTA and PC showed comparative biocompatibility while evaluated in-vitro.
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spelling pubmed-38447612013-12-02 Cytotoxicity Evaluation of Proroot MTA, Root MTA and Portland Cement on Human Gingival Fibroblasts Sharifian, Mohammad Reza Ghobadi, Mehrnoosh Shokouhinejad, Noushin Assadian, Hadi Iran Endod J Original Article INTRODUCTION: The aim of this study was to compare the cytotoxicity of ProRoot MTA (PMTA), Root MTA (RMTA) and Portland cement (PC) on human gingival fibroblasts (HGFs). MATERIALS AND METHODS: The extracts of the test materials were placed close to human gingival fibroblasts (HGFs) within 96-well plates. Cellular viability was assessed using MTT assay in different intervals (freshly mixed, 4, 24, and 168 hours after mixing). The data were analyzed using the One-way ANOVA and Tukey’s test at 95% significance level. RESULTS: It was indicated that there was not a significant difference in cytotoxicity of test materials (p>0.05). In addition, there was not a statistically significant difference between different time intervals within each group (p>0.05). CONCLUSION: PMTA, RMTA and PC showed comparative biocompatibility while evaluated in-vitro. Iranian Center for Endodontic Research 2007 2007-10-02 /pmc/articles/PMC3844761/ /pubmed/24298288 Text en © 2007, Iranian Center for Endodontic Research This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Sharifian, Mohammad Reza
Ghobadi, Mehrnoosh
Shokouhinejad, Noushin
Assadian, Hadi
Cytotoxicity Evaluation of Proroot MTA, Root MTA and Portland Cement on Human Gingival Fibroblasts
title Cytotoxicity Evaluation of Proroot MTA, Root MTA and Portland Cement on Human Gingival Fibroblasts
title_full Cytotoxicity Evaluation of Proroot MTA, Root MTA and Portland Cement on Human Gingival Fibroblasts
title_fullStr Cytotoxicity Evaluation of Proroot MTA, Root MTA and Portland Cement on Human Gingival Fibroblasts
title_full_unstemmed Cytotoxicity Evaluation of Proroot MTA, Root MTA and Portland Cement on Human Gingival Fibroblasts
title_short Cytotoxicity Evaluation of Proroot MTA, Root MTA and Portland Cement on Human Gingival Fibroblasts
title_sort cytotoxicity evaluation of proroot mta, root mta and portland cement on human gingival fibroblasts
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3844761/
https://www.ncbi.nlm.nih.gov/pubmed/24298288
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