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Force Measurement During Contraction to Assess Muscle Function in Zebrafish Larvae

Zebrafish larvae provide models of muscle development, muscle disease and muscle-related chemical toxicity, but related studies often lack functional measures of muscle health. In this video article, we demonstrate a method to measure force generation during contraction of zebrafish larval trunk mus...

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Autores principales: Sloboda, Darcée D., Claflin, Dennis R., Dowling, James J., Brooks, Susan V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3846141/
https://www.ncbi.nlm.nih.gov/pubmed/23912162
http://dx.doi.org/10.3791/50539
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author Sloboda, Darcée D.
Claflin, Dennis R.
Dowling, James J.
Brooks, Susan V.
author_facet Sloboda, Darcée D.
Claflin, Dennis R.
Dowling, James J.
Brooks, Susan V.
author_sort Sloboda, Darcée D.
collection PubMed
description Zebrafish larvae provide models of muscle development, muscle disease and muscle-related chemical toxicity, but related studies often lack functional measures of muscle health. In this video article, we demonstrate a method to measure force generation during contraction of zebrafish larval trunk muscle. Force measurements are accomplished by placing an anesthetized larva into a chamber filled with a salt solution. The anterior end of the larva is tied to a force transducer and the posterior end of the larva is tied to a length controller. An isometric twitch contraction is elicited by electric field stimulation and the force response is recorded for analysis. Force generation during contraction provides a measure of overall muscle health and specifically provides a measure of muscle function. Although we describe this technique for use with wild-type larvae, this method can be used with genetically modified larvae or with larvae treated with drugs or toxicants, to characterize muscle disease models and evaluate treatments, or to study muscle development, injury, or chemical toxicity.
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spelling pubmed-38461412014-05-08 Force Measurement During Contraction to Assess Muscle Function in Zebrafish Larvae Sloboda, Darcée D. Claflin, Dennis R. Dowling, James J. Brooks, Susan V. J Vis Exp Developmental Biology Zebrafish larvae provide models of muscle development, muscle disease and muscle-related chemical toxicity, but related studies often lack functional measures of muscle health. In this video article, we demonstrate a method to measure force generation during contraction of zebrafish larval trunk muscle. Force measurements are accomplished by placing an anesthetized larva into a chamber filled with a salt solution. The anterior end of the larva is tied to a force transducer and the posterior end of the larva is tied to a length controller. An isometric twitch contraction is elicited by electric field stimulation and the force response is recorded for analysis. Force generation during contraction provides a measure of overall muscle health and specifically provides a measure of muscle function. Although we describe this technique for use with wild-type larvae, this method can be used with genetically modified larvae or with larvae treated with drugs or toxicants, to characterize muscle disease models and evaluate treatments, or to study muscle development, injury, or chemical toxicity. MyJove Corporation 2013-07-23 /pmc/articles/PMC3846141/ /pubmed/23912162 http://dx.doi.org/10.3791/50539 Text en Copyright © 2013, Journal of Visualized Experiments http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visithttp://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Developmental Biology
Sloboda, Darcée D.
Claflin, Dennis R.
Dowling, James J.
Brooks, Susan V.
Force Measurement During Contraction to Assess Muscle Function in Zebrafish Larvae
title Force Measurement During Contraction to Assess Muscle Function in Zebrafish Larvae
title_full Force Measurement During Contraction to Assess Muscle Function in Zebrafish Larvae
title_fullStr Force Measurement During Contraction to Assess Muscle Function in Zebrafish Larvae
title_full_unstemmed Force Measurement During Contraction to Assess Muscle Function in Zebrafish Larvae
title_short Force Measurement During Contraction to Assess Muscle Function in Zebrafish Larvae
title_sort force measurement during contraction to assess muscle function in zebrafish larvae
topic Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3846141/
https://www.ncbi.nlm.nih.gov/pubmed/23912162
http://dx.doi.org/10.3791/50539
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