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Phylodynamic Profile of HIV-1 Subtype B, CRF01_AE and the Recently Emerging CRF51_01B among Men Who Have Sex with Men (MSM) in Singapore

HIV-1 subtype B and CRF01_AE are the predominant infecting subtypes among men who have sex with men (MSM) in Singapore. The genetic history, population dynamics and pattern of transmission networks of these genotypes remain largely unknown. We delineated the phylodynamic profiles of HIV-1 subtype B,...

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Autores principales: Ng, Kim Tien, Ng, Kah Ying, Khong, Wei Xin, Chew, Kuan Kiat, Singh, Palvinder Kaur, Yap, Joe Kwan, Tan, Mei Ting, Leo, Yee Sin, Laeyendecker, Oliver, Quinn, Thomas C., Kamarulzaman, Adeeba, Tee, Kok Keng, Ng, Oon Tek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3846621/
https://www.ncbi.nlm.nih.gov/pubmed/24312505
http://dx.doi.org/10.1371/journal.pone.0080884
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author Ng, Kim Tien
Ng, Kah Ying
Khong, Wei Xin
Chew, Kuan Kiat
Singh, Palvinder Kaur
Yap, Joe Kwan
Tan, Mei Ting
Leo, Yee Sin
Laeyendecker, Oliver
Quinn, Thomas C.
Kamarulzaman, Adeeba
Tee, Kok Keng
Ng, Oon Tek
author_facet Ng, Kim Tien
Ng, Kah Ying
Khong, Wei Xin
Chew, Kuan Kiat
Singh, Palvinder Kaur
Yap, Joe Kwan
Tan, Mei Ting
Leo, Yee Sin
Laeyendecker, Oliver
Quinn, Thomas C.
Kamarulzaman, Adeeba
Tee, Kok Keng
Ng, Oon Tek
author_sort Ng, Kim Tien
collection PubMed
description HIV-1 subtype B and CRF01_AE are the predominant infecting subtypes among men who have sex with men (MSM) in Singapore. The genetic history, population dynamics and pattern of transmission networks of these genotypes remain largely unknown. We delineated the phylodynamic profiles of HIV-1 subtype B, CRF01_AE and the recently characterized CRF51_01B strains circulating among the MSM population in Singapore. A total of 105 (49.5%) newly-diagnosed treatment-naïve MSM were recruited between February 2008 and August 2009. Phylogenetic reconstructions of the protease gene (HXB2: 2239 – 2629), gp120 (HXB2: 6942 – 7577) and gp41 (HXB2: 7803 – 8276) of the env gene uncovered five monophyletic transmission networks (two each within subtype B and CRF01_AE and one within CRF51_01B lineages) of different sizes (involving 3 – 23 MSM subjects, supported by posterior probability measure of 1.0). Bayesian coalescent analysis estimated that the emergence and dissemination of multiple sub-epidemic networks occurred between 1995 and 2005, driven largely by subtype B and later followed by CRF01_AE. Exponential increase in effective population size for both subtype B and CRF01_AE occurred between 2002 to 2007 and 2005 to 2007, respectively. Genealogical estimates suggested that the novel CRF51_01B lineages were probably generated through series of recombination events involving CRF01_AE and multiple subtype B ancestors. Our study provides the first insight on the phylodynamic profiles of HIV-1 subtype B, CRF01_AE and CRF51_01B viral strains circulating among MSM in Singapore.
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spelling pubmed-38466212013-12-05 Phylodynamic Profile of HIV-1 Subtype B, CRF01_AE and the Recently Emerging CRF51_01B among Men Who Have Sex with Men (MSM) in Singapore Ng, Kim Tien Ng, Kah Ying Khong, Wei Xin Chew, Kuan Kiat Singh, Palvinder Kaur Yap, Joe Kwan Tan, Mei Ting Leo, Yee Sin Laeyendecker, Oliver Quinn, Thomas C. Kamarulzaman, Adeeba Tee, Kok Keng Ng, Oon Tek PLoS One Research Article HIV-1 subtype B and CRF01_AE are the predominant infecting subtypes among men who have sex with men (MSM) in Singapore. The genetic history, population dynamics and pattern of transmission networks of these genotypes remain largely unknown. We delineated the phylodynamic profiles of HIV-1 subtype B, CRF01_AE and the recently characterized CRF51_01B strains circulating among the MSM population in Singapore. A total of 105 (49.5%) newly-diagnosed treatment-naïve MSM were recruited between February 2008 and August 2009. Phylogenetic reconstructions of the protease gene (HXB2: 2239 – 2629), gp120 (HXB2: 6942 – 7577) and gp41 (HXB2: 7803 – 8276) of the env gene uncovered five monophyletic transmission networks (two each within subtype B and CRF01_AE and one within CRF51_01B lineages) of different sizes (involving 3 – 23 MSM subjects, supported by posterior probability measure of 1.0). Bayesian coalescent analysis estimated that the emergence and dissemination of multiple sub-epidemic networks occurred between 1995 and 2005, driven largely by subtype B and later followed by CRF01_AE. Exponential increase in effective population size for both subtype B and CRF01_AE occurred between 2002 to 2007 and 2005 to 2007, respectively. Genealogical estimates suggested that the novel CRF51_01B lineages were probably generated through series of recombination events involving CRF01_AE and multiple subtype B ancestors. Our study provides the first insight on the phylodynamic profiles of HIV-1 subtype B, CRF01_AE and CRF51_01B viral strains circulating among MSM in Singapore. Public Library of Science 2013-12-02 /pmc/articles/PMC3846621/ /pubmed/24312505 http://dx.doi.org/10.1371/journal.pone.0080884 Text en © 2013 Ng et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ng, Kim Tien
Ng, Kah Ying
Khong, Wei Xin
Chew, Kuan Kiat
Singh, Palvinder Kaur
Yap, Joe Kwan
Tan, Mei Ting
Leo, Yee Sin
Laeyendecker, Oliver
Quinn, Thomas C.
Kamarulzaman, Adeeba
Tee, Kok Keng
Ng, Oon Tek
Phylodynamic Profile of HIV-1 Subtype B, CRF01_AE and the Recently Emerging CRF51_01B among Men Who Have Sex with Men (MSM) in Singapore
title Phylodynamic Profile of HIV-1 Subtype B, CRF01_AE and the Recently Emerging CRF51_01B among Men Who Have Sex with Men (MSM) in Singapore
title_full Phylodynamic Profile of HIV-1 Subtype B, CRF01_AE and the Recently Emerging CRF51_01B among Men Who Have Sex with Men (MSM) in Singapore
title_fullStr Phylodynamic Profile of HIV-1 Subtype B, CRF01_AE and the Recently Emerging CRF51_01B among Men Who Have Sex with Men (MSM) in Singapore
title_full_unstemmed Phylodynamic Profile of HIV-1 Subtype B, CRF01_AE and the Recently Emerging CRF51_01B among Men Who Have Sex with Men (MSM) in Singapore
title_short Phylodynamic Profile of HIV-1 Subtype B, CRF01_AE and the Recently Emerging CRF51_01B among Men Who Have Sex with Men (MSM) in Singapore
title_sort phylodynamic profile of hiv-1 subtype b, crf01_ae and the recently emerging crf51_01b among men who have sex with men (msm) in singapore
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3846621/
https://www.ncbi.nlm.nih.gov/pubmed/24312505
http://dx.doi.org/10.1371/journal.pone.0080884
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