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Subunit Organisation of In Vitro Reconstituted HOPS and CORVET Multisubunit Membrane Tethering Complexes

Biochemical and structural analysis of macromolecular protein assemblies remains challenging due to technical difficulties in recombinant expression, engineering and reconstitution of multisubunit complexes. Here we use a recently developed cell-free protein expression system based on the protozoan...

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Detalles Bibliográficos
Autores principales: Guo, Zhong, Johnston, Wayne, Kovtun, Oleksiy, Mureev, Sergey, Bröcker, Cornelia, Ungermann, Christian, Alexandrov, Kirill
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3846719/
https://www.ncbi.nlm.nih.gov/pubmed/24312556
http://dx.doi.org/10.1371/journal.pone.0081534