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Robust 3D DNA FISH Using Directly Labeled Probes
3D DNA FISH has become a major tool for analyzing three-dimensional organization of the nucleus, and several variations of the technique have been published. In this article we describe a protocol which has been optimized for robustness, reproducibility, and ease of use. Brightly fluorescent directl...
Autores principales: | Bolland, Daniel J., King, Michelle R., Reik, Wolf, Corcoran, Anne E., Krueger, Christel |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MyJove Corporation
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3846859/ https://www.ncbi.nlm.nih.gov/pubmed/23978815 http://dx.doi.org/10.3791/50587 |
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