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Histopathological and clinical evaluation of Kombucha tea and Nitrofurazone on cutaneous full-thickness wounds healing in rats: an experimental study

BACKGROUND: Kombucha, a fermented tea (KT) is claimed to possess many beneficial properties. The aim of this study was to evaluate clinical and histopathological alterations of Kombucha tea and Nitrofurazone on cutaneous full-thickness wounds healing in rat. METHODS: In present study 24 Wister -albi...

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Autores principales: Barati, Fardin, Javanbakht, Javad, Adib-Hashemi, Farajollah, Hosseini, Ehsan, Safaeie, Reyhaneh, Rajabian, Mojtaba, Razmjoo, Mostafa, Sedaghat, Reza, Aghamohammad Hassan, Mehdi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3846920/
https://www.ncbi.nlm.nih.gov/pubmed/23866960
http://dx.doi.org/10.1186/1746-1596-8-120
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author Barati, Fardin
Javanbakht, Javad
Adib-Hashemi, Farajollah
Hosseini, Ehsan
Safaeie, Reyhaneh
Rajabian, Mojtaba
Razmjoo, Mostafa
Sedaghat, Reza
Aghamohammad Hassan, Mehdi
author_facet Barati, Fardin
Javanbakht, Javad
Adib-Hashemi, Farajollah
Hosseini, Ehsan
Safaeie, Reyhaneh
Rajabian, Mojtaba
Razmjoo, Mostafa
Sedaghat, Reza
Aghamohammad Hassan, Mehdi
author_sort Barati, Fardin
collection PubMed
description BACKGROUND: Kombucha, a fermented tea (KT) is claimed to possess many beneficial properties. The aim of this study was to evaluate clinical and histopathological alterations of Kombucha tea and Nitrofurazone on cutaneous full-thickness wounds healing in rat. METHODS: In present study 24 Wister -albino rats weighing 150–200 g were selected and divided to two treatment groups as Nitrofurazone ointment (0.2%) and Kombucha tea. Subsequently, the anesthesia was exerted by Ketamin hydrochloride 10% (40 mg/kg) and Xylasine (2 mg/kg) through intra muscular (IM) route. Furthermore, upon preparation of dorsal region of the animal for surgery, a piece of full-thickness skin removed (2 × 2 cm). In order to comparing wounds healing clinically and histologically, once every four days from the commencement, the wounds were photographed and the healed surface was measured by Scion image software. RESULT: The clinical findings indicated that the Kombucha fungus resulted in precipitating healing than Nitrofurazone; however, it was not significant (p > 0.05). In order to pathological comparing of wound healing process, several wound biopsies were taken on 4, 8, 12, 16 and 20(th) days. Additionally, the histopathological results demonstrated that there was inflammation in Nitrofurazone group through twelveth day, somehow the epithelium was formed and abundant vessels were visible. Although on 16(th) day and the previous days the healing condition of Kombucha fungus was considered as minimal rate, revealing it is similar to Nitrofurazone group on 20(th) day. CONCLUSIONS: To wrap up. These observations suggest that the Kombucha fungus healing quality was rapid from 12(th) day to the end of the research, whereas no significant difference was observed. VIRTUAL SLIDE: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1107407136102196
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spelling pubmed-38469202013-12-04 Histopathological and clinical evaluation of Kombucha tea and Nitrofurazone on cutaneous full-thickness wounds healing in rats: an experimental study Barati, Fardin Javanbakht, Javad Adib-Hashemi, Farajollah Hosseini, Ehsan Safaeie, Reyhaneh Rajabian, Mojtaba Razmjoo, Mostafa Sedaghat, Reza Aghamohammad Hassan, Mehdi Diagn Pathol Research BACKGROUND: Kombucha, a fermented tea (KT) is claimed to possess many beneficial properties. The aim of this study was to evaluate clinical and histopathological alterations of Kombucha tea and Nitrofurazone on cutaneous full-thickness wounds healing in rat. METHODS: In present study 24 Wister -albino rats weighing 150–200 g were selected and divided to two treatment groups as Nitrofurazone ointment (0.2%) and Kombucha tea. Subsequently, the anesthesia was exerted by Ketamin hydrochloride 10% (40 mg/kg) and Xylasine (2 mg/kg) through intra muscular (IM) route. Furthermore, upon preparation of dorsal region of the animal for surgery, a piece of full-thickness skin removed (2 × 2 cm). In order to comparing wounds healing clinically and histologically, once every four days from the commencement, the wounds were photographed and the healed surface was measured by Scion image software. RESULT: The clinical findings indicated that the Kombucha fungus resulted in precipitating healing than Nitrofurazone; however, it was not significant (p > 0.05). In order to pathological comparing of wound healing process, several wound biopsies were taken on 4, 8, 12, 16 and 20(th) days. Additionally, the histopathological results demonstrated that there was inflammation in Nitrofurazone group through twelveth day, somehow the epithelium was formed and abundant vessels were visible. Although on 16(th) day and the previous days the healing condition of Kombucha fungus was considered as minimal rate, revealing it is similar to Nitrofurazone group on 20(th) day. CONCLUSIONS: To wrap up. These observations suggest that the Kombucha fungus healing quality was rapid from 12(th) day to the end of the research, whereas no significant difference was observed. VIRTUAL SLIDE: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1107407136102196 BioMed Central 2013-07-17 /pmc/articles/PMC3846920/ /pubmed/23866960 http://dx.doi.org/10.1186/1746-1596-8-120 Text en Copyright © 2013 Barati et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Barati, Fardin
Javanbakht, Javad
Adib-Hashemi, Farajollah
Hosseini, Ehsan
Safaeie, Reyhaneh
Rajabian, Mojtaba
Razmjoo, Mostafa
Sedaghat, Reza
Aghamohammad Hassan, Mehdi
Histopathological and clinical evaluation of Kombucha tea and Nitrofurazone on cutaneous full-thickness wounds healing in rats: an experimental study
title Histopathological and clinical evaluation of Kombucha tea and Nitrofurazone on cutaneous full-thickness wounds healing in rats: an experimental study
title_full Histopathological and clinical evaluation of Kombucha tea and Nitrofurazone on cutaneous full-thickness wounds healing in rats: an experimental study
title_fullStr Histopathological and clinical evaluation of Kombucha tea and Nitrofurazone on cutaneous full-thickness wounds healing in rats: an experimental study
title_full_unstemmed Histopathological and clinical evaluation of Kombucha tea and Nitrofurazone on cutaneous full-thickness wounds healing in rats: an experimental study
title_short Histopathological and clinical evaluation of Kombucha tea and Nitrofurazone on cutaneous full-thickness wounds healing in rats: an experimental study
title_sort histopathological and clinical evaluation of kombucha tea and nitrofurazone on cutaneous full-thickness wounds healing in rats: an experimental study
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3846920/
https://www.ncbi.nlm.nih.gov/pubmed/23866960
http://dx.doi.org/10.1186/1746-1596-8-120
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