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Bimolecular Fluorescence Complementation; Lighting-Up Tau-Tau Interaction in Living Cells
Abnormal tau aggregation is a pathological hallmark of many neurodegenerative disorders and it is becoming apparent that soluble tau aggregates play a key role in neurodegeneration and memory impairment. Despite this pathological importance, there is currently no single method that allows monitoring...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3847076/ https://www.ncbi.nlm.nih.gov/pubmed/24312574 http://dx.doi.org/10.1371/journal.pone.0081682 |
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author | Tak, HyeJin Haque, Md. Mamunul Kim, Min Jung Lee, Joo Hyun Baik, Ja-Hyun Kim, YoungSoo Kim, Dong Jin Grailhe, Regis Kim, Yun Kyung |
author_facet | Tak, HyeJin Haque, Md. Mamunul Kim, Min Jung Lee, Joo Hyun Baik, Ja-Hyun Kim, YoungSoo Kim, Dong Jin Grailhe, Regis Kim, Yun Kyung |
author_sort | Tak, HyeJin |
collection | PubMed |
description | Abnormal tau aggregation is a pathological hallmark of many neurodegenerative disorders and it is becoming apparent that soluble tau aggregates play a key role in neurodegeneration and memory impairment. Despite this pathological importance, there is currently no single method that allows monitoring soluble tau species in living cells. In this regard, we developed a cell-based sensor that visualizes tau self-assembly. By introducing bimolecular fluorescence complementation (BiFC) technique to tau, we were able to achieve spatial and temporal resolution of tau-tau interactions in a range of states, from soluble dimers to large aggregates. Under basal conditions, tau-BiFC cells exhibited little fluorescence intensity, implying that the majority of tau molecules exist as monomers. Upon chemically induced tau hyperphosphorylation, BiFC fluorescence greatly increased, indicating an increased level of tau-tau interactions. As an indicator of tau assembly, our BiFC sensor would be a useful tool for investigating tau pathology. |
format | Online Article Text |
id | pubmed-3847076 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-38470762013-12-05 Bimolecular Fluorescence Complementation; Lighting-Up Tau-Tau Interaction in Living Cells Tak, HyeJin Haque, Md. Mamunul Kim, Min Jung Lee, Joo Hyun Baik, Ja-Hyun Kim, YoungSoo Kim, Dong Jin Grailhe, Regis Kim, Yun Kyung PLoS One Research Article Abnormal tau aggregation is a pathological hallmark of many neurodegenerative disorders and it is becoming apparent that soluble tau aggregates play a key role in neurodegeneration and memory impairment. Despite this pathological importance, there is currently no single method that allows monitoring soluble tau species in living cells. In this regard, we developed a cell-based sensor that visualizes tau self-assembly. By introducing bimolecular fluorescence complementation (BiFC) technique to tau, we were able to achieve spatial and temporal resolution of tau-tau interactions in a range of states, from soluble dimers to large aggregates. Under basal conditions, tau-BiFC cells exhibited little fluorescence intensity, implying that the majority of tau molecules exist as monomers. Upon chemically induced tau hyperphosphorylation, BiFC fluorescence greatly increased, indicating an increased level of tau-tau interactions. As an indicator of tau assembly, our BiFC sensor would be a useful tool for investigating tau pathology. Public Library of Science 2013-12-02 /pmc/articles/PMC3847076/ /pubmed/24312574 http://dx.doi.org/10.1371/journal.pone.0081682 Text en © 2013 Tak et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Tak, HyeJin Haque, Md. Mamunul Kim, Min Jung Lee, Joo Hyun Baik, Ja-Hyun Kim, YoungSoo Kim, Dong Jin Grailhe, Regis Kim, Yun Kyung Bimolecular Fluorescence Complementation; Lighting-Up Tau-Tau Interaction in Living Cells |
title | Bimolecular Fluorescence Complementation; Lighting-Up Tau-Tau Interaction in Living Cells |
title_full | Bimolecular Fluorescence Complementation; Lighting-Up Tau-Tau Interaction in Living Cells |
title_fullStr | Bimolecular Fluorescence Complementation; Lighting-Up Tau-Tau Interaction in Living Cells |
title_full_unstemmed | Bimolecular Fluorescence Complementation; Lighting-Up Tau-Tau Interaction in Living Cells |
title_short | Bimolecular Fluorescence Complementation; Lighting-Up Tau-Tau Interaction in Living Cells |
title_sort | bimolecular fluorescence complementation; lighting-up tau-tau interaction in living cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3847076/ https://www.ncbi.nlm.nih.gov/pubmed/24312574 http://dx.doi.org/10.1371/journal.pone.0081682 |
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