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Discovery of chlamydial peptidoglycan reveals bacteria with murein sacculi but without FtsZ

Chlamydiae are important pathogens and symbionts, with unique cell biology features. They lack the cell-division protein FtsZ, which functions in maintaining cell shape and orchestrating cell division in almost all other bacteria. In addition, the existence of peptidoglycan (PG) in chlamydial cell e...

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Autores principales: Pilhofer, Martin, Aistleitner, Karin, Biboy, Jacob, Gray, Joe, Kuru, Erkin, Hall, Edward, Brun, Yves V., VanNieuwenhze, Michael S., Vollmer, Waldemar, Horn, Matthias, Jensen, Grant J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3847603/
https://www.ncbi.nlm.nih.gov/pubmed/24292151
http://dx.doi.org/10.1038/ncomms3856
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author Pilhofer, Martin
Aistleitner, Karin
Biboy, Jacob
Gray, Joe
Kuru, Erkin
Hall, Edward
Brun, Yves V.
VanNieuwenhze, Michael S.
Vollmer, Waldemar
Horn, Matthias
Jensen, Grant J.
author_facet Pilhofer, Martin
Aistleitner, Karin
Biboy, Jacob
Gray, Joe
Kuru, Erkin
Hall, Edward
Brun, Yves V.
VanNieuwenhze, Michael S.
Vollmer, Waldemar
Horn, Matthias
Jensen, Grant J.
author_sort Pilhofer, Martin
collection PubMed
description Chlamydiae are important pathogens and symbionts, with unique cell biology features. They lack the cell-division protein FtsZ, which functions in maintaining cell shape and orchestrating cell division in almost all other bacteria. In addition, the existence of peptidoglycan (PG) in chlamydial cell envelopes has been highly controversial. Using electron cryotomography, mass spectrometry and fluorescent labeling dyes, here we show that some environmental chlamydiae have cell-wall sacculi consisting of an unusual PG type. Treatment with fosfomycin (a PG synthesis inhibitor) leads to lower infection rates and aberrant cell shapes, suggesting that PG synthesis is crucial for the chlamydial life cycle. Our findings demonstrate for the first time the presence of PG in a member of the Chlamydiae. They also present a unique example of a bacterium with a PG sacculus but without FtsZ, challenging the current hypothesis that it is the absence of a cell wall that renders FtsZ non-essential.
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spelling pubmed-38476032014-06-02 Discovery of chlamydial peptidoglycan reveals bacteria with murein sacculi but without FtsZ Pilhofer, Martin Aistleitner, Karin Biboy, Jacob Gray, Joe Kuru, Erkin Hall, Edward Brun, Yves V. VanNieuwenhze, Michael S. Vollmer, Waldemar Horn, Matthias Jensen, Grant J. Nat Commun Article Chlamydiae are important pathogens and symbionts, with unique cell biology features. They lack the cell-division protein FtsZ, which functions in maintaining cell shape and orchestrating cell division in almost all other bacteria. In addition, the existence of peptidoglycan (PG) in chlamydial cell envelopes has been highly controversial. Using electron cryotomography, mass spectrometry and fluorescent labeling dyes, here we show that some environmental chlamydiae have cell-wall sacculi consisting of an unusual PG type. Treatment with fosfomycin (a PG synthesis inhibitor) leads to lower infection rates and aberrant cell shapes, suggesting that PG synthesis is crucial for the chlamydial life cycle. Our findings demonstrate for the first time the presence of PG in a member of the Chlamydiae. They also present a unique example of a bacterium with a PG sacculus but without FtsZ, challenging the current hypothesis that it is the absence of a cell wall that renders FtsZ non-essential. 2013-12-02 /pmc/articles/PMC3847603/ /pubmed/24292151 http://dx.doi.org/10.1038/ncomms3856 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Pilhofer, Martin
Aistleitner, Karin
Biboy, Jacob
Gray, Joe
Kuru, Erkin
Hall, Edward
Brun, Yves V.
VanNieuwenhze, Michael S.
Vollmer, Waldemar
Horn, Matthias
Jensen, Grant J.
Discovery of chlamydial peptidoglycan reveals bacteria with murein sacculi but without FtsZ
title Discovery of chlamydial peptidoglycan reveals bacteria with murein sacculi but without FtsZ
title_full Discovery of chlamydial peptidoglycan reveals bacteria with murein sacculi but without FtsZ
title_fullStr Discovery of chlamydial peptidoglycan reveals bacteria with murein sacculi but without FtsZ
title_full_unstemmed Discovery of chlamydial peptidoglycan reveals bacteria with murein sacculi but without FtsZ
title_short Discovery of chlamydial peptidoglycan reveals bacteria with murein sacculi but without FtsZ
title_sort discovery of chlamydial peptidoglycan reveals bacteria with murein sacculi but without ftsz
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3847603/
https://www.ncbi.nlm.nih.gov/pubmed/24292151
http://dx.doi.org/10.1038/ncomms3856
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