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Simultaneous splicing of multiple DNA fragments in one PCR reaction

BACKGROUND: Rapid and simultaneous splicing of multiple DNA fragments is frequently required in many recombinant DNA projects. However, former overlap extension PCRs, the most common methods for splicing DNA fragments, are not really simultaneous fusing of multiple DNA fragments. RESULTS: We perform...

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Detalles Bibliográficos
Autores principales:	Luo, Wei-Gui, Liu, Hui-Zhen, Lin, Wan-Huang, Kabir, Mohammed Humayun, Su, Yi
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2013
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3847634/ https://www.ncbi.nlm.nih.gov/pubmed/24015676 http://dx.doi.org/10.1186/1480-9222-15-9

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