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Effects of Vitamin D(3), Calcipotriol and FTY720 on the Expression of Surface Molecules and Cytolytic Activities of Human Natural Killer Cells and Dendritic Cells

We describe here the effects of three drugs that are either approved or have the potential for treating multiple sclerosis (MS) patients through the in vitro activities of human natural killer (NK) cells and dendritic cells (DCs). Our results indicate that 1,25(OH)(2)D(3), the biologically active me...

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Autores principales: Al-Jaderi, Zaidoon, Maghazachi, Azzam A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3847708/
https://www.ncbi.nlm.nih.gov/pubmed/24169587
http://dx.doi.org/10.3390/toxins5111932
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author Al-Jaderi, Zaidoon
Maghazachi, Azzam A.
author_facet Al-Jaderi, Zaidoon
Maghazachi, Azzam A.
author_sort Al-Jaderi, Zaidoon
collection PubMed
description We describe here the effects of three drugs that are either approved or have the potential for treating multiple sclerosis (MS) patients through the in vitro activities of human natural killer (NK) cells and dendritic cells (DCs). Our results indicate that 1,25(OH)(2)D(3), the biologically active metabolite vitamin D(3), calcipotriol and FTY720 augment IL-2-activated NK cell lysis of K562 and RAJI tumor cell lines as well as immature (i) and mature (m) DCs, with variable efficacies. These results are corroborated with the ability of the drugs to up-regulate the expression of NK cytotoxicity receptors NKp30 and NKp44, as well as NKG2D on the surfaces of NK cells. Also, they down-regulate the expression of the killer inhibitory receptor CD158. The three drugs down-regulate the expression of CCR6 on the surface of iDCs, whereas vitamin D(3) and calcipotriol tend to up-regulate the expression of CCR7 on mDCs, suggesting that they may influence the migration of DCs into the lymph nodes. Finally, vitamin D(3), calcipotriol and FTY720 enhance NK17/NK1 cell lysis of K562 cells, suggesting that a possible mechanism of action for these drugs is via activating these newly described cells. In conclusion, our results show novel mechanisms of action for vitamin D(3), calcipotriol and FTY720 on cells of the innate immune system.
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spelling pubmed-38477082013-12-03 Effects of Vitamin D(3), Calcipotriol and FTY720 on the Expression of Surface Molecules and Cytolytic Activities of Human Natural Killer Cells and Dendritic Cells Al-Jaderi, Zaidoon Maghazachi, Azzam A. Toxins (Basel) Article We describe here the effects of three drugs that are either approved or have the potential for treating multiple sclerosis (MS) patients through the in vitro activities of human natural killer (NK) cells and dendritic cells (DCs). Our results indicate that 1,25(OH)(2)D(3), the biologically active metabolite vitamin D(3), calcipotriol and FTY720 augment IL-2-activated NK cell lysis of K562 and RAJI tumor cell lines as well as immature (i) and mature (m) DCs, with variable efficacies. These results are corroborated with the ability of the drugs to up-regulate the expression of NK cytotoxicity receptors NKp30 and NKp44, as well as NKG2D on the surfaces of NK cells. Also, they down-regulate the expression of the killer inhibitory receptor CD158. The three drugs down-regulate the expression of CCR6 on the surface of iDCs, whereas vitamin D(3) and calcipotriol tend to up-regulate the expression of CCR7 on mDCs, suggesting that they may influence the migration of DCs into the lymph nodes. Finally, vitamin D(3), calcipotriol and FTY720 enhance NK17/NK1 cell lysis of K562 cells, suggesting that a possible mechanism of action for these drugs is via activating these newly described cells. In conclusion, our results show novel mechanisms of action for vitamin D(3), calcipotriol and FTY720 on cells of the innate immune system. MDPI 2013-10-28 /pmc/articles/PMC3847708/ /pubmed/24169587 http://dx.doi.org/10.3390/toxins5111932 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Al-Jaderi, Zaidoon
Maghazachi, Azzam A.
Effects of Vitamin D(3), Calcipotriol and FTY720 on the Expression of Surface Molecules and Cytolytic Activities of Human Natural Killer Cells and Dendritic Cells
title Effects of Vitamin D(3), Calcipotriol and FTY720 on the Expression of Surface Molecules and Cytolytic Activities of Human Natural Killer Cells and Dendritic Cells
title_full Effects of Vitamin D(3), Calcipotriol and FTY720 on the Expression of Surface Molecules and Cytolytic Activities of Human Natural Killer Cells and Dendritic Cells
title_fullStr Effects of Vitamin D(3), Calcipotriol and FTY720 on the Expression of Surface Molecules and Cytolytic Activities of Human Natural Killer Cells and Dendritic Cells
title_full_unstemmed Effects of Vitamin D(3), Calcipotriol and FTY720 on the Expression of Surface Molecules and Cytolytic Activities of Human Natural Killer Cells and Dendritic Cells
title_short Effects of Vitamin D(3), Calcipotriol and FTY720 on the Expression of Surface Molecules and Cytolytic Activities of Human Natural Killer Cells and Dendritic Cells
title_sort effects of vitamin d(3), calcipotriol and fty720 on the expression of surface molecules and cytolytic activities of human natural killer cells and dendritic cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3847708/
https://www.ncbi.nlm.nih.gov/pubmed/24169587
http://dx.doi.org/10.3390/toxins5111932
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