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Non-Viral Delivery and Therapeutic Application of Small Interfering RNAs

RNA interference (RNAi) is a powerful method used for gene expression regulation. The increasing knowledge about the molecular mechanism of this phenomenon creates new avenues for the application of the RNAi technology in the treatment of various human diseases. However, delivery of RNA interference...

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Detalles Bibliográficos
Autores principales: Nikitenko, N. A., Prassolov, V. S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: A.I. Gordeyev 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3848066/
https://www.ncbi.nlm.nih.gov/pubmed/24303201
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author Nikitenko, N. A.
Prassolov, V. S.
author_facet Nikitenko, N. A.
Prassolov, V. S.
author_sort Nikitenko, N. A.
collection PubMed
description RNA interference (RNAi) is a powerful method used for gene expression regulation. The increasing knowledge about the molecular mechanism of this phenomenon creates new avenues for the application of the RNAi technology in the treatment of various human diseases. However, delivery of RNA interference mediators, small interfering RNAs (siRNAs), to target cells is a major hurdle. Effective and safe pharmacological use of siRNAs requires carriers that can deliver siRNA to its target site and the development of methods for protection of these fragile molecules from in vivo degradation. This review summarizes various strategies for siRNA delivery, including chemical modification and non-viral approaches, such as the polymer-based, peptide-based, lipid-based techniques, and inorganic nanosystems. The advantages, disadvantages, and prospects for the therapeutic application of these methods are also examined in this paper.
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spelling pubmed-38480662013-12-03 Non-Viral Delivery and Therapeutic Application of Small Interfering RNAs Nikitenko, N. A. Prassolov, V. S. Acta Naturae Research Article RNA interference (RNAi) is a powerful method used for gene expression regulation. The increasing knowledge about the molecular mechanism of this phenomenon creates new avenues for the application of the RNAi technology in the treatment of various human diseases. However, delivery of RNA interference mediators, small interfering RNAs (siRNAs), to target cells is a major hurdle. Effective and safe pharmacological use of siRNAs requires carriers that can deliver siRNA to its target site and the development of methods for protection of these fragile molecules from in vivo degradation. This review summarizes various strategies for siRNA delivery, including chemical modification and non-viral approaches, such as the polymer-based, peptide-based, lipid-based techniques, and inorganic nanosystems. The advantages, disadvantages, and prospects for the therapeutic application of these methods are also examined in this paper. A.I. Gordeyev 2013 /pmc/articles/PMC3848066/ /pubmed/24303201 Text en Copyright © 2013 Park-media Ltd. http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Nikitenko, N. A.
Prassolov, V. S.
Non-Viral Delivery and Therapeutic Application of Small Interfering RNAs
title Non-Viral Delivery and Therapeutic Application of Small Interfering RNAs
title_full Non-Viral Delivery and Therapeutic Application of Small Interfering RNAs
title_fullStr Non-Viral Delivery and Therapeutic Application of Small Interfering RNAs
title_full_unstemmed Non-Viral Delivery and Therapeutic Application of Small Interfering RNAs
title_short Non-Viral Delivery and Therapeutic Application of Small Interfering RNAs
title_sort non-viral delivery and therapeutic application of small interfering rnas
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3848066/
https://www.ncbi.nlm.nih.gov/pubmed/24303201
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