Cargando…

Parthenium hysterophorus: A Probable Source of Anticancer, Antioxidant and Anti-HIV Agents

The present work reports the anticancer, antioxidant, lipo-protective, and anti-HIV activities of phytoconstituents present in P. hysterophorus leaf. Dried leaf samples were sequentially extracted with nonpolar and polar solvents. Ethanol fraction showed noticeable cytotoxic activity (81–85%) in SRB...

Descripción completa

Detalles Bibliográficos
Autores principales: Kumar, Shashank, Chashoo, Gousia, Saxena, Ajit K., Pandey, Abhay K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3848086/
https://www.ncbi.nlm.nih.gov/pubmed/24350290
http://dx.doi.org/10.1155/2013/810734
_version_ 1782293716667465728
author Kumar, Shashank
Chashoo, Gousia
Saxena, Ajit K.
Pandey, Abhay K.
author_facet Kumar, Shashank
Chashoo, Gousia
Saxena, Ajit K.
Pandey, Abhay K.
author_sort Kumar, Shashank
collection PubMed
description The present work reports the anticancer, antioxidant, lipo-protective, and anti-HIV activities of phytoconstituents present in P. hysterophorus leaf. Dried leaf samples were sequentially extracted with nonpolar and polar solvents. Ethanol fraction showed noticeable cytotoxic activity (81–85%) in SRB assay against MCF-7 and THP-1 cancer cell lines at 100 μg/ml concentration, while lower activity was observed with DU-145 cell line. The same extract exhibited 17–98% growth inhibition of HL-60 cancer cell lines in MTT assay, showing concentration dependent response. Ethanol extract caused 12% reduction in mitochondrial membrane potential and 10% increment in sub G1 population of HL-60 cell lines. Several leaf fractions, namely, ethyl acetate, ethanol, and aqueous fractions exhibited considerable reducing capability at higher concentrations. Most of the extracts demonstrated appreciable (>75%) metal ion chelating and hydroxyl radical scavenging activities at 200 µg/ml. All the extracts except aqueous fraction accounted for about 70–80% inhibition of lipid peroxidation in rat liver homogenate indicating protective response against membrane damage. About 40% inhibition of reverse transcriptase (RT) activity was observed in hexane fraction in anti-HIV assay at 6.0 µg/ml concentration. The study showed that phytochemicals present in P. hysterophorus leaf have considerable potential as cytotoxic and antioxidant agents with low to moderate anti-HIV activity.
format Online
Article
Text
id pubmed-3848086
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-38480862013-12-12 Parthenium hysterophorus: A Probable Source of Anticancer, Antioxidant and Anti-HIV Agents Kumar, Shashank Chashoo, Gousia Saxena, Ajit K. Pandey, Abhay K. Biomed Res Int Research Article The present work reports the anticancer, antioxidant, lipo-protective, and anti-HIV activities of phytoconstituents present in P. hysterophorus leaf. Dried leaf samples were sequentially extracted with nonpolar and polar solvents. Ethanol fraction showed noticeable cytotoxic activity (81–85%) in SRB assay against MCF-7 and THP-1 cancer cell lines at 100 μg/ml concentration, while lower activity was observed with DU-145 cell line. The same extract exhibited 17–98% growth inhibition of HL-60 cancer cell lines in MTT assay, showing concentration dependent response. Ethanol extract caused 12% reduction in mitochondrial membrane potential and 10% increment in sub G1 population of HL-60 cell lines. Several leaf fractions, namely, ethyl acetate, ethanol, and aqueous fractions exhibited considerable reducing capability at higher concentrations. Most of the extracts demonstrated appreciable (>75%) metal ion chelating and hydroxyl radical scavenging activities at 200 µg/ml. All the extracts except aqueous fraction accounted for about 70–80% inhibition of lipid peroxidation in rat liver homogenate indicating protective response against membrane damage. About 40% inhibition of reverse transcriptase (RT) activity was observed in hexane fraction in anti-HIV assay at 6.0 µg/ml concentration. The study showed that phytochemicals present in P. hysterophorus leaf have considerable potential as cytotoxic and antioxidant agents with low to moderate anti-HIV activity. Hindawi Publishing Corporation 2013 2013-11-17 /pmc/articles/PMC3848086/ /pubmed/24350290 http://dx.doi.org/10.1155/2013/810734 Text en Copyright © 2013 Shashank Kumar et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kumar, Shashank
Chashoo, Gousia
Saxena, Ajit K.
Pandey, Abhay K.
Parthenium hysterophorus: A Probable Source of Anticancer, Antioxidant and Anti-HIV Agents
title Parthenium hysterophorus: A Probable Source of Anticancer, Antioxidant and Anti-HIV Agents
title_full Parthenium hysterophorus: A Probable Source of Anticancer, Antioxidant and Anti-HIV Agents
title_fullStr Parthenium hysterophorus: A Probable Source of Anticancer, Antioxidant and Anti-HIV Agents
title_full_unstemmed Parthenium hysterophorus: A Probable Source of Anticancer, Antioxidant and Anti-HIV Agents
title_short Parthenium hysterophorus: A Probable Source of Anticancer, Antioxidant and Anti-HIV Agents
title_sort parthenium hysterophorus: a probable source of anticancer, antioxidant and anti-hiv agents
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3848086/
https://www.ncbi.nlm.nih.gov/pubmed/24350290
http://dx.doi.org/10.1155/2013/810734
work_keys_str_mv AT kumarshashank partheniumhysterophorusaprobablesourceofanticancerantioxidantandantihivagents
AT chashoogousia partheniumhysterophorusaprobablesourceofanticancerantioxidantandantihivagents
AT saxenaajitk partheniumhysterophorusaprobablesourceofanticancerantioxidantandantihivagents
AT pandeyabhayk partheniumhysterophorusaprobablesourceofanticancerantioxidantandantihivagents