Cargando…

Resolution of five-component mixture using mean centering ratio and inverse least squares chemometrics

BACKGROUND: A comparative study of the use of mean centering of ratio spectra and inverse least squares for the resolution of paracetamol, methylparaben, propylparaben, chlorpheniramine maleate and pseudoephedrine hydrochloride has been achieved showing that the two chemometric methods provide a goo...

Descripción completa

Detalles Bibliográficos
Autores principales: Issa, Mahmoud Mohamed, Nejem, R’afat Mahmoud, Shanab, Alaa Mohamed Abu, Shaat, Nahed Talab
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3848590/
https://www.ncbi.nlm.nih.gov/pubmed/24028626
http://dx.doi.org/10.1186/1752-153X-7-152
_version_ 1782293785792741376
author Issa, Mahmoud Mohamed
Nejem, R’afat Mahmoud
Shanab, Alaa Mohamed Abu
Shaat, Nahed Talab
author_facet Issa, Mahmoud Mohamed
Nejem, R’afat Mahmoud
Shanab, Alaa Mohamed Abu
Shaat, Nahed Talab
author_sort Issa, Mahmoud Mohamed
collection PubMed
description BACKGROUND: A comparative study of the use of mean centering of ratio spectra and inverse least squares for the resolution of paracetamol, methylparaben, propylparaben, chlorpheniramine maleate and pseudoephedrine hydrochloride has been achieved showing that the two chemometric methods provide a good example of the high resolving power of these techniques. Method (I) is the mean centering of ratio spectra which depends on using the mean centered ratio spectra in four successive steps that eliminates the derivative steps and therefore the signal to noise ratio is improved. The absorption spectra of prepared solutions were measured in the range of 220–280 nm. Method (II) is based on the inverse least squares that depend on updating developed multivariate calibration model. The absorption spectra of the prepared mixtures in the range 230–270 nm were recorded. RESULTS: The linear concentration ranges were 0–25.6, 0–15.0, 0–15.0, 0–45.0 and 0–100.0 μg mL(-1) for paracetamol, methylparaben, propylparaben, chlorpheniramine maleate and pseudoephedrine hydrochloride, respectively. The mean recoveries for simultaneous determination were between 99.9-101.3% for the two methods. The two developed methods have been successfully used for prediction of five-component mixture in Decamol Flu syrup with good selectivity, high sensitivity and extremely low detection limit. CONCLUSION: No published method has been reported for simultaneous determination of the five components of this mixture so that the results of the mean centering of ratio spectra method were compared with those of the proposed inverse least squares method. Statistical comparison was performed using t-test and F-ratio at P = 0.05. There was no significant difference between the results.
format Online
Article
Text
id pubmed-3848590
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-38485902013-12-05 Resolution of five-component mixture using mean centering ratio and inverse least squares chemometrics Issa, Mahmoud Mohamed Nejem, R’afat Mahmoud Shanab, Alaa Mohamed Abu Shaat, Nahed Talab Chem Cent J Research Article BACKGROUND: A comparative study of the use of mean centering of ratio spectra and inverse least squares for the resolution of paracetamol, methylparaben, propylparaben, chlorpheniramine maleate and pseudoephedrine hydrochloride has been achieved showing that the two chemometric methods provide a good example of the high resolving power of these techniques. Method (I) is the mean centering of ratio spectra which depends on using the mean centered ratio spectra in four successive steps that eliminates the derivative steps and therefore the signal to noise ratio is improved. The absorption spectra of prepared solutions were measured in the range of 220–280 nm. Method (II) is based on the inverse least squares that depend on updating developed multivariate calibration model. The absorption spectra of the prepared mixtures in the range 230–270 nm were recorded. RESULTS: The linear concentration ranges were 0–25.6, 0–15.0, 0–15.0, 0–45.0 and 0–100.0 μg mL(-1) for paracetamol, methylparaben, propylparaben, chlorpheniramine maleate and pseudoephedrine hydrochloride, respectively. The mean recoveries for simultaneous determination were between 99.9-101.3% for the two methods. The two developed methods have been successfully used for prediction of five-component mixture in Decamol Flu syrup with good selectivity, high sensitivity and extremely low detection limit. CONCLUSION: No published method has been reported for simultaneous determination of the five components of this mixture so that the results of the mean centering of ratio spectra method were compared with those of the proposed inverse least squares method. Statistical comparison was performed using t-test and F-ratio at P = 0.05. There was no significant difference between the results. BioMed Central 2013-09-12 /pmc/articles/PMC3848590/ /pubmed/24028626 http://dx.doi.org/10.1186/1752-153X-7-152 Text en Copyright © 2013 Issa et al.; licensee Chemistry Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Issa, Mahmoud Mohamed
Nejem, R’afat Mahmoud
Shanab, Alaa Mohamed Abu
Shaat, Nahed Talab
Resolution of five-component mixture using mean centering ratio and inverse least squares chemometrics
title Resolution of five-component mixture using mean centering ratio and inverse least squares chemometrics
title_full Resolution of five-component mixture using mean centering ratio and inverse least squares chemometrics
title_fullStr Resolution of five-component mixture using mean centering ratio and inverse least squares chemometrics
title_full_unstemmed Resolution of five-component mixture using mean centering ratio and inverse least squares chemometrics
title_short Resolution of five-component mixture using mean centering ratio and inverse least squares chemometrics
title_sort resolution of five-component mixture using mean centering ratio and inverse least squares chemometrics
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3848590/
https://www.ncbi.nlm.nih.gov/pubmed/24028626
http://dx.doi.org/10.1186/1752-153X-7-152
work_keys_str_mv AT issamahmoudmohamed resolutionoffivecomponentmixtureusingmeancenteringratioandinverseleastsquareschemometrics
AT nejemrafatmahmoud resolutionoffivecomponentmixtureusingmeancenteringratioandinverseleastsquareschemometrics
AT shanabalaamohamedabu resolutionoffivecomponentmixtureusingmeancenteringratioandinverseleastsquareschemometrics
AT shaatnahedtalab resolutionoffivecomponentmixtureusingmeancenteringratioandinverseleastsquareschemometrics