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Reference loci for RT-qPCR analysis of differentiating human embryonic stem cells

BACKGROUND: Selecting stably expressed reference genes is essential for proper reverse transcription quantitative polymerase chain reaction gene expression analysis. However, this choice is not always straightforward. In the case of differentiating human embryonic stem (hES) cells, differentiation i...

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Detalles Bibliográficos
Autores principales: Vossaert, Liesbeth, O’Leary, Thomas, Van Neste, Christophe, Heindryckx, Björn, Vandesompele, Jo, De Sutter, Petra, Deforce, Dieter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3848990/
https://www.ncbi.nlm.nih.gov/pubmed/24028740
http://dx.doi.org/10.1186/1471-2199-14-21