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Spectrophotometric detection of susceptibility to anti-malarial drugs

BACKGROUND: With malaria drug resistance increasing in prevalence and severity, new technologies are needed to aid and improve the accuracy and clinical relevance of laboratory or field testing for malaria drug resistance. This study presents a method based on simple and reagentless spectroscopic me...

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Autores principales: Serebrennikova, Yulia M, Patel, Janus, Milhous, Wilbur K, Garcia-Rubio, Luis H, Huffman, Debra E, Smith, Jennifer M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3849014/
https://www.ncbi.nlm.nih.gov/pubmed/23992478
http://dx.doi.org/10.1186/1475-2875-12-305
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author Serebrennikova, Yulia M
Patel, Janus
Milhous, Wilbur K
Garcia-Rubio, Luis H
Huffman, Debra E
Smith, Jennifer M
author_facet Serebrennikova, Yulia M
Patel, Janus
Milhous, Wilbur K
Garcia-Rubio, Luis H
Huffman, Debra E
Smith, Jennifer M
author_sort Serebrennikova, Yulia M
collection PubMed
description BACKGROUND: With malaria drug resistance increasing in prevalence and severity, new technologies are needed to aid and improve the accuracy and clinical relevance of laboratory or field testing for malaria drug resistance. This study presents a method based on simple and reagentless spectroscopic measurements coupled with comprehensive spectral interpretation analysis that provides valuable quantitative information on the morphological and compositional responses of Plasmodium falciparum and infected red blood cells (IRBCs) to anti-malarial treatment. METHODS: The changes in the size, internal structure, nucleotide and haemozoin composition of the parasites as well as the morphology (size and shape) and haemoglobin composition of the IRBCs treated with dihydroartemisinin (DHA) and mefloquine (MFQ) were investigated using a spectral interpretation analysis. RESULTS: DHA treatment reduced the sizes of the parasites and their structural organelles. The haemoglobin composition of the host IRBCs determined from spectroscopic analysis changed negligibly following DHA treatment. MFQ treated parasites grew to the same size as those from parallel non-treated cultures but lacked haemozoin. Lesser deformation of the cell shape and no haemoglobin depletion were detected for the IRBCs of MFQ treated cultures. CONCLUSIONS: The spectroscopic analysis method proved to be sensitive for recognition of the effects of anti-malarial treatment on the structure and composition of the parasites and IRBCs. The method can have significant potential for research and clinical applications such as evaluating patient specimens for drug action, drug effects or for therapeutic monitoring.
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spelling pubmed-38490142013-12-07 Spectrophotometric detection of susceptibility to anti-malarial drugs Serebrennikova, Yulia M Patel, Janus Milhous, Wilbur K Garcia-Rubio, Luis H Huffman, Debra E Smith, Jennifer M Malar J Research BACKGROUND: With malaria drug resistance increasing in prevalence and severity, new technologies are needed to aid and improve the accuracy and clinical relevance of laboratory or field testing for malaria drug resistance. This study presents a method based on simple and reagentless spectroscopic measurements coupled with comprehensive spectral interpretation analysis that provides valuable quantitative information on the morphological and compositional responses of Plasmodium falciparum and infected red blood cells (IRBCs) to anti-malarial treatment. METHODS: The changes in the size, internal structure, nucleotide and haemozoin composition of the parasites as well as the morphology (size and shape) and haemoglobin composition of the IRBCs treated with dihydroartemisinin (DHA) and mefloquine (MFQ) were investigated using a spectral interpretation analysis. RESULTS: DHA treatment reduced the sizes of the parasites and their structural organelles. The haemoglobin composition of the host IRBCs determined from spectroscopic analysis changed negligibly following DHA treatment. MFQ treated parasites grew to the same size as those from parallel non-treated cultures but lacked haemozoin. Lesser deformation of the cell shape and no haemoglobin depletion were detected for the IRBCs of MFQ treated cultures. CONCLUSIONS: The spectroscopic analysis method proved to be sensitive for recognition of the effects of anti-malarial treatment on the structure and composition of the parasites and IRBCs. The method can have significant potential for research and clinical applications such as evaluating patient specimens for drug action, drug effects or for therapeutic monitoring. BioMed Central 2013-08-30 /pmc/articles/PMC3849014/ /pubmed/23992478 http://dx.doi.org/10.1186/1475-2875-12-305 Text en Copyright © 2013 Serebrennikova et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Serebrennikova, Yulia M
Patel, Janus
Milhous, Wilbur K
Garcia-Rubio, Luis H
Huffman, Debra E
Smith, Jennifer M
Spectrophotometric detection of susceptibility to anti-malarial drugs
title Spectrophotometric detection of susceptibility to anti-malarial drugs
title_full Spectrophotometric detection of susceptibility to anti-malarial drugs
title_fullStr Spectrophotometric detection of susceptibility to anti-malarial drugs
title_full_unstemmed Spectrophotometric detection of susceptibility to anti-malarial drugs
title_short Spectrophotometric detection of susceptibility to anti-malarial drugs
title_sort spectrophotometric detection of susceptibility to anti-malarial drugs
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3849014/
https://www.ncbi.nlm.nih.gov/pubmed/23992478
http://dx.doi.org/10.1186/1475-2875-12-305
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