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Nuclear lipid droplets identified by electron microscopy of serial sections

BACKGROUND: Recent studies have suggested that nuclear lipid droplets (LDs) are organized into domains similar to those of cytoplasmic LDs. As cytoplasmic LDs are formed at the endoplasmic reticulum (ER) membrane, which is structurally continuous with the nuclear envelope, it could be suggested howe...

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Autores principales: Uzbekov, Rustem, Roingeard, Philippe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3849021/
https://www.ncbi.nlm.nih.gov/pubmed/24070407
http://dx.doi.org/10.1186/1756-0500-6-386
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author Uzbekov, Rustem
Roingeard, Philippe
author_facet Uzbekov, Rustem
Roingeard, Philippe
author_sort Uzbekov, Rustem
collection PubMed
description BACKGROUND: Recent studies have suggested that nuclear lipid droplets (LDs) are organized into domains similar to those of cytoplasmic LDs. As cytoplasmic LDs are formed at the endoplasmic reticulum (ER) membrane, which is structurally continuous with the nuclear envelope, it could be suggested however that nuclear LDs are cytoplamic LDs trapped within an invagination of the nuclear envelope. The resolution of fluorescence confocal microscopy is not sufficiently high to exclude this hypothesis. FINDINGS: We therefore addressed this question by electron microscopy (EM) of serial sections. In human liver tissue, we observed some cytoplamic LDs partly surrounded by the nuclear compartment, but we were also able to identify LDs residing in the nuclear compartment that were not connected to the nuclear envelope. CONCLUSION: These findings indicate that nuclear LDs constitute specific subdomains of the nuclear compartment probably involved in nuclear lipid homeostasis.
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spelling pubmed-38490212013-12-04 Nuclear lipid droplets identified by electron microscopy of serial sections Uzbekov, Rustem Roingeard, Philippe BMC Res Notes Short Report BACKGROUND: Recent studies have suggested that nuclear lipid droplets (LDs) are organized into domains similar to those of cytoplasmic LDs. As cytoplasmic LDs are formed at the endoplasmic reticulum (ER) membrane, which is structurally continuous with the nuclear envelope, it could be suggested however that nuclear LDs are cytoplamic LDs trapped within an invagination of the nuclear envelope. The resolution of fluorescence confocal microscopy is not sufficiently high to exclude this hypothesis. FINDINGS: We therefore addressed this question by electron microscopy (EM) of serial sections. In human liver tissue, we observed some cytoplamic LDs partly surrounded by the nuclear compartment, but we were also able to identify LDs residing in the nuclear compartment that were not connected to the nuclear envelope. CONCLUSION: These findings indicate that nuclear LDs constitute specific subdomains of the nuclear compartment probably involved in nuclear lipid homeostasis. BioMed Central 2013-09-27 /pmc/articles/PMC3849021/ /pubmed/24070407 http://dx.doi.org/10.1186/1756-0500-6-386 Text en Copyright © 2013 Uzbekov and Roingeard; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Report
Uzbekov, Rustem
Roingeard, Philippe
Nuclear lipid droplets identified by electron microscopy of serial sections
title Nuclear lipid droplets identified by electron microscopy of serial sections
title_full Nuclear lipid droplets identified by electron microscopy of serial sections
title_fullStr Nuclear lipid droplets identified by electron microscopy of serial sections
title_full_unstemmed Nuclear lipid droplets identified by electron microscopy of serial sections
title_short Nuclear lipid droplets identified by electron microscopy of serial sections
title_sort nuclear lipid droplets identified by electron microscopy of serial sections
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3849021/
https://www.ncbi.nlm.nih.gov/pubmed/24070407
http://dx.doi.org/10.1186/1756-0500-6-386
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