Cargando…
The Nedd4-binding protein 3 (N4BP3) is crucial for axonal and dendritic branching in developing neurons
BACKGROUND: Circuit formation in the nervous system essentially relies on the proper development of neurons and their processes. In this context, the ubiquitin ligase Nedd4 is a crucial modulator of axonal and dendritic branching. RESULTS: Herein we characterize the Nedd4-binding protein 3 (N4BP3),...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3849298/ https://www.ncbi.nlm.nih.gov/pubmed/24044555 http://dx.doi.org/10.1186/1749-8104-8-18 |
_version_ | 1782293904185360384 |
---|---|
author | Schmeisser, Michael J Kühl, Susanne J Schoen, Michael Beth, Nathalie H Weis, Tobias M Grabrucker, Andreas M Kühl, Michael Boeckers, Tobias M |
author_facet | Schmeisser, Michael J Kühl, Susanne J Schoen, Michael Beth, Nathalie H Weis, Tobias M Grabrucker, Andreas M Kühl, Michael Boeckers, Tobias M |
author_sort | Schmeisser, Michael J |
collection | PubMed |
description | BACKGROUND: Circuit formation in the nervous system essentially relies on the proper development of neurons and their processes. In this context, the ubiquitin ligase Nedd4 is a crucial modulator of axonal and dendritic branching. RESULTS: Herein we characterize the Nedd4-binding protein 3 (N4BP3), a Fezzin family member, during nerve cell development. In developing rat primary hippocampal neurons, endogenous N4BP3 localizes to neuronal processes, including axons and dendrites. Transient in vitro knockdown of N4BP3 in hippocampal cultures during neuritogenesis results in impaired branching of axons and dendrites. In line with these findings, in vivo knockdown of n4bp3 in Xenopus laevis embryos results in severe alteration of cranial nerve branching. CONCLUSIONS: We introduce N4BP3 as a novel molecular element for the correct branching of neurites in developing neurons and propose a central role for an N4BP3-Nedd4 complex in neurite branching and circuit formation. |
format | Online Article Text |
id | pubmed-3849298 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-38492982013-12-05 The Nedd4-binding protein 3 (N4BP3) is crucial for axonal and dendritic branching in developing neurons Schmeisser, Michael J Kühl, Susanne J Schoen, Michael Beth, Nathalie H Weis, Tobias M Grabrucker, Andreas M Kühl, Michael Boeckers, Tobias M Neural Dev Research Article BACKGROUND: Circuit formation in the nervous system essentially relies on the proper development of neurons and their processes. In this context, the ubiquitin ligase Nedd4 is a crucial modulator of axonal and dendritic branching. RESULTS: Herein we characterize the Nedd4-binding protein 3 (N4BP3), a Fezzin family member, during nerve cell development. In developing rat primary hippocampal neurons, endogenous N4BP3 localizes to neuronal processes, including axons and dendrites. Transient in vitro knockdown of N4BP3 in hippocampal cultures during neuritogenesis results in impaired branching of axons and dendrites. In line with these findings, in vivo knockdown of n4bp3 in Xenopus laevis embryos results in severe alteration of cranial nerve branching. CONCLUSIONS: We introduce N4BP3 as a novel molecular element for the correct branching of neurites in developing neurons and propose a central role for an N4BP3-Nedd4 complex in neurite branching and circuit formation. BioMed Central 2013-09-17 /pmc/articles/PMC3849298/ /pubmed/24044555 http://dx.doi.org/10.1186/1749-8104-8-18 Text en Copyright © 2013 Schmeisser et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Schmeisser, Michael J Kühl, Susanne J Schoen, Michael Beth, Nathalie H Weis, Tobias M Grabrucker, Andreas M Kühl, Michael Boeckers, Tobias M The Nedd4-binding protein 3 (N4BP3) is crucial for axonal and dendritic branching in developing neurons |
title | The Nedd4-binding protein 3 (N4BP3) is crucial for axonal and dendritic branching in developing neurons |
title_full | The Nedd4-binding protein 3 (N4BP3) is crucial for axonal and dendritic branching in developing neurons |
title_fullStr | The Nedd4-binding protein 3 (N4BP3) is crucial for axonal and dendritic branching in developing neurons |
title_full_unstemmed | The Nedd4-binding protein 3 (N4BP3) is crucial for axonal and dendritic branching in developing neurons |
title_short | The Nedd4-binding protein 3 (N4BP3) is crucial for axonal and dendritic branching in developing neurons |
title_sort | nedd4-binding protein 3 (n4bp3) is crucial for axonal and dendritic branching in developing neurons |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3849298/ https://www.ncbi.nlm.nih.gov/pubmed/24044555 http://dx.doi.org/10.1186/1749-8104-8-18 |
work_keys_str_mv | AT schmeissermichaelj thenedd4bindingprotein3n4bp3iscrucialforaxonalanddendriticbranchingindevelopingneurons AT kuhlsusannej thenedd4bindingprotein3n4bp3iscrucialforaxonalanddendriticbranchingindevelopingneurons AT schoenmichael thenedd4bindingprotein3n4bp3iscrucialforaxonalanddendriticbranchingindevelopingneurons AT bethnathalieh thenedd4bindingprotein3n4bp3iscrucialforaxonalanddendriticbranchingindevelopingneurons AT weistobiasm thenedd4bindingprotein3n4bp3iscrucialforaxonalanddendriticbranchingindevelopingneurons AT grabruckerandreasm thenedd4bindingprotein3n4bp3iscrucialforaxonalanddendriticbranchingindevelopingneurons AT kuhlmichael thenedd4bindingprotein3n4bp3iscrucialforaxonalanddendriticbranchingindevelopingneurons AT boeckerstobiasm thenedd4bindingprotein3n4bp3iscrucialforaxonalanddendriticbranchingindevelopingneurons AT schmeissermichaelj nedd4bindingprotein3n4bp3iscrucialforaxonalanddendriticbranchingindevelopingneurons AT kuhlsusannej nedd4bindingprotein3n4bp3iscrucialforaxonalanddendriticbranchingindevelopingneurons AT schoenmichael nedd4bindingprotein3n4bp3iscrucialforaxonalanddendriticbranchingindevelopingneurons AT bethnathalieh nedd4bindingprotein3n4bp3iscrucialforaxonalanddendriticbranchingindevelopingneurons AT weistobiasm nedd4bindingprotein3n4bp3iscrucialforaxonalanddendriticbranchingindevelopingneurons AT grabruckerandreasm nedd4bindingprotein3n4bp3iscrucialforaxonalanddendriticbranchingindevelopingneurons AT kuhlmichael nedd4bindingprotein3n4bp3iscrucialforaxonalanddendriticbranchingindevelopingneurons AT boeckerstobiasm nedd4bindingprotein3n4bp3iscrucialforaxonalanddendriticbranchingindevelopingneurons |