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Transcriptome Analysis Identifies Regulators of Hematopoietic Stem and Progenitor Cells

Hematopoietic stem cells (HSCs) maintain blood homeostasis and are the functional units of bone marrow transplantation. To improve the molecular understanding of HSCs and their proximal progenitors, we performed transcriptome analysis within the context of the ImmGen Consortium data set. Gene sets t...

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Detalles Bibliográficos
Autores principales: Gazit, Roi, Garrison, Brian S., Rao, Tata Nageswara, Shay, Tal, Costello, James, Ericson, Jeff, Kim, Francis, Collins, James J., Regev, Aviv, Wagers, Amy J., Rossi, Derrick J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3849420/
https://www.ncbi.nlm.nih.gov/pubmed/24319662
http://dx.doi.org/10.1016/j.stemcr.2013.07.004
Descripción
Sumario:Hematopoietic stem cells (HSCs) maintain blood homeostasis and are the functional units of bone marrow transplantation. To improve the molecular understanding of HSCs and their proximal progenitors, we performed transcriptome analysis within the context of the ImmGen Consortium data set. Gene sets that define steady-state and mobilized HSCs, as well as hematopoietic stem and progenitor cells (HSPCs), were determined. Genes involved in transcriptional regulation, including a group of putative transcriptional repressors, were identified in multipotent progenitors and HSCs. Proximal promoter analyses combined with ImmGen module analysis identified candidate regulators of HSCs. Enforced expression of one predicted regulator, Hlf, in diverse HSPC subsets led to extensive self-renewal activity ex vivo. These analyses reveal unique insights into the mechanisms that control the core properties of HSPCs.