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Study on the antiviral activity of San Huang Yi Gan Capsule against hepatitis B virus with seropharmacological method

BACKGROUND: Seropharmacology arising recently is a novel method of in vitro pharmacological study on Chinese herb using drug-containing animal serum. As seropharmacology possesses the advantages of experiments in vitro and in vivo, it is increasingly applied in pharmacological research on Chinese me...

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Autores principales: Xu, Haibo, Wu, Qinghe, Peng, Cheng, Zhou, Lijuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3849434/
https://www.ncbi.nlm.nih.gov/pubmed/24073917
http://dx.doi.org/10.1186/1472-6882-13-239
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author Xu, Haibo
Wu, Qinghe
Peng, Cheng
Zhou, Lijuan
author_facet Xu, Haibo
Wu, Qinghe
Peng, Cheng
Zhou, Lijuan
author_sort Xu, Haibo
collection PubMed
description BACKGROUND: Seropharmacology arising recently is a novel method of in vitro pharmacological study on Chinese herb using drug-containing animal serum. As seropharmacology possesses the advantages of experiments in vitro and in vivo, it is increasingly applied in pharmacological research on Chinese medicine. However, some issues of seropharmacology remain controversial and need to be clearly defined. San Huang Yi Gan Capsule (SHYGC) is a Chinese herbal formula with antiviral property against hepatitis B virus (HBV), but little is known about the mechanism underlying its anti-HBV activity. The aim of the present study was to elucidate the action mechanism of SHYGC using seropharmacological method and systematically address the methodology of preparing drug-containing serum. METHODS: New Zealand rabbits were orally administrated SHYGC with various regimens, followed by preparation of SHYGC-containing rabbit sera with a variety of methods. After HBV-producing HepG2 2.2.15 cells were treated with SHYGC-containing sera or entecavir for 9 days, the levels of hepatitis B surface antigen (HBsAg) and HBV DNA and the activity of DNA Polymerase were determined in HepG2 2.2.15 cells-conditioned media. RESULTS: An optimally standardized method of preparing drug-containing serum was raised for seropharmacology, with which SHYGC was demonstrated to suppress HBsAg expression, HBV DNA replication and DNA Polymerase activity in a dose-dependent fashion. CONCLUSIONS: This seropharmacological study shows SHYGC is a potentially powerful anti-HBV agent. Additionally, seropharmacology is a promising pharmacological method with a broad range of advantages, and it can be widely used in biomedical research, if combined with pharmacokinetics.
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spelling pubmed-38494342013-12-05 Study on the antiviral activity of San Huang Yi Gan Capsule against hepatitis B virus with seropharmacological method Xu, Haibo Wu, Qinghe Peng, Cheng Zhou, Lijuan BMC Complement Altern Med Research Article BACKGROUND: Seropharmacology arising recently is a novel method of in vitro pharmacological study on Chinese herb using drug-containing animal serum. As seropharmacology possesses the advantages of experiments in vitro and in vivo, it is increasingly applied in pharmacological research on Chinese medicine. However, some issues of seropharmacology remain controversial and need to be clearly defined. San Huang Yi Gan Capsule (SHYGC) is a Chinese herbal formula with antiviral property against hepatitis B virus (HBV), but little is known about the mechanism underlying its anti-HBV activity. The aim of the present study was to elucidate the action mechanism of SHYGC using seropharmacological method and systematically address the methodology of preparing drug-containing serum. METHODS: New Zealand rabbits were orally administrated SHYGC with various regimens, followed by preparation of SHYGC-containing rabbit sera with a variety of methods. After HBV-producing HepG2 2.2.15 cells were treated with SHYGC-containing sera or entecavir for 9 days, the levels of hepatitis B surface antigen (HBsAg) and HBV DNA and the activity of DNA Polymerase were determined in HepG2 2.2.15 cells-conditioned media. RESULTS: An optimally standardized method of preparing drug-containing serum was raised for seropharmacology, with which SHYGC was demonstrated to suppress HBsAg expression, HBV DNA replication and DNA Polymerase activity in a dose-dependent fashion. CONCLUSIONS: This seropharmacological study shows SHYGC is a potentially powerful anti-HBV agent. Additionally, seropharmacology is a promising pharmacological method with a broad range of advantages, and it can be widely used in biomedical research, if combined with pharmacokinetics. BioMed Central 2013-09-27 /pmc/articles/PMC3849434/ /pubmed/24073917 http://dx.doi.org/10.1186/1472-6882-13-239 Text en Copyright © 2013 Xu et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Xu, Haibo
Wu, Qinghe
Peng, Cheng
Zhou, Lijuan
Study on the antiviral activity of San Huang Yi Gan Capsule against hepatitis B virus with seropharmacological method
title Study on the antiviral activity of San Huang Yi Gan Capsule against hepatitis B virus with seropharmacological method
title_full Study on the antiviral activity of San Huang Yi Gan Capsule against hepatitis B virus with seropharmacological method
title_fullStr Study on the antiviral activity of San Huang Yi Gan Capsule against hepatitis B virus with seropharmacological method
title_full_unstemmed Study on the antiviral activity of San Huang Yi Gan Capsule against hepatitis B virus with seropharmacological method
title_short Study on the antiviral activity of San Huang Yi Gan Capsule against hepatitis B virus with seropharmacological method
title_sort study on the antiviral activity of san huang yi gan capsule against hepatitis b virus with seropharmacological method
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3849434/
https://www.ncbi.nlm.nih.gov/pubmed/24073917
http://dx.doi.org/10.1186/1472-6882-13-239
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