Cargando…

Oncostatin M stimulates cell migration and proliferation by down-regulating E-cadherin in HTR8/SVneo cell line through STAT3 activation

BACKGROUND: During the first trimester of pregnancy, trophoblastic E-cadherin expression is down-regulated, thereby allowing extravillous trophoblasts (EVTs) to acquire the potential for migration and invasiveness. The aim of the present study was to investigate the role of OSM on the migration and...

Descripción completa

Detalles Bibliográficos
Autores principales: Ko, Hyun Sun, Choi, Sae Kyung, Kang, Hee Kyung, Kim, Ho Shik, Jeon, Ji Hyun, Park, In Yang, Shin, Jong Chul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3849455/
https://www.ncbi.nlm.nih.gov/pubmed/24060241
http://dx.doi.org/10.1186/1477-7827-11-93
_version_ 1782293928864645120
author Ko, Hyun Sun
Choi, Sae Kyung
Kang, Hee Kyung
Kim, Ho Shik
Jeon, Ji Hyun
Park, In Yang
Shin, Jong Chul
author_facet Ko, Hyun Sun
Choi, Sae Kyung
Kang, Hee Kyung
Kim, Ho Shik
Jeon, Ji Hyun
Park, In Yang
Shin, Jong Chul
author_sort Ko, Hyun Sun
collection PubMed
description BACKGROUND: During the first trimester of pregnancy, trophoblastic E-cadherin expression is down-regulated, thereby allowing extravillous trophoblasts (EVTs) to acquire the potential for migration and invasiveness. The aim of the present study was to investigate the role of OSM on the migration and proliferation of EVT cell line HTR8/SVneo with regard to its effects on the expression of E-cadherin and STAT3 activation. METHODS: We investigated the effects of OSM on RNA and protein expression of E-cadherin by real time RT-PCR analyses, western blotting, and indirect immunofluorescence staining in HTR8/SVneo cells, as well as the effects on cell migration and proliferation. The selective signal transducer and activator of transcription (STAT)3 inhibitor, stattic, and STAT3 siRNA were used to investigate STAT3 activation by OSM. RESULTS: OSM significantly reduced RNA and protein expression of E-cadherin. Indirect immunofluorescence staining of HTR8/SVneo cells also revealed the down-regulation of E-cadherin, compared with the controls. OSM-stimulated cell migration was attenuated by anti-gp130 antibodies. OSM-induced STAT3 phosphorylation, and the down-regulation of E-cadherin by OSM treatment was restored by stattic and STAT3 siRNA. In addition, OSM-stimulated migration and proliferation were significantly suppressed by STAT3 inhibition. CONCLUSIONS: This study suggests that OSM stimulates the migration and proliferation of EVTs during the first trimester of pregnancy through the down-regulation of E-cadherin. In addition, this study suggests that the effects of OSM on migration and proliferation are related to STAT3 activation, which is important in trophoblast invasiveness.
format Online
Article
Text
id pubmed-3849455
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-38494552013-12-05 Oncostatin M stimulates cell migration and proliferation by down-regulating E-cadherin in HTR8/SVneo cell line through STAT3 activation Ko, Hyun Sun Choi, Sae Kyung Kang, Hee Kyung Kim, Ho Shik Jeon, Ji Hyun Park, In Yang Shin, Jong Chul Reprod Biol Endocrinol Research BACKGROUND: During the first trimester of pregnancy, trophoblastic E-cadherin expression is down-regulated, thereby allowing extravillous trophoblasts (EVTs) to acquire the potential for migration and invasiveness. The aim of the present study was to investigate the role of OSM on the migration and proliferation of EVT cell line HTR8/SVneo with regard to its effects on the expression of E-cadherin and STAT3 activation. METHODS: We investigated the effects of OSM on RNA and protein expression of E-cadherin by real time RT-PCR analyses, western blotting, and indirect immunofluorescence staining in HTR8/SVneo cells, as well as the effects on cell migration and proliferation. The selective signal transducer and activator of transcription (STAT)3 inhibitor, stattic, and STAT3 siRNA were used to investigate STAT3 activation by OSM. RESULTS: OSM significantly reduced RNA and protein expression of E-cadherin. Indirect immunofluorescence staining of HTR8/SVneo cells also revealed the down-regulation of E-cadherin, compared with the controls. OSM-stimulated cell migration was attenuated by anti-gp130 antibodies. OSM-induced STAT3 phosphorylation, and the down-regulation of E-cadherin by OSM treatment was restored by stattic and STAT3 siRNA. In addition, OSM-stimulated migration and proliferation were significantly suppressed by STAT3 inhibition. CONCLUSIONS: This study suggests that OSM stimulates the migration and proliferation of EVTs during the first trimester of pregnancy through the down-regulation of E-cadherin. In addition, this study suggests that the effects of OSM on migration and proliferation are related to STAT3 activation, which is important in trophoblast invasiveness. BioMed Central 2013-09-24 /pmc/articles/PMC3849455/ /pubmed/24060241 http://dx.doi.org/10.1186/1477-7827-11-93 Text en Copyright © 2013 Ko et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Ko, Hyun Sun
Choi, Sae Kyung
Kang, Hee Kyung
Kim, Ho Shik
Jeon, Ji Hyun
Park, In Yang
Shin, Jong Chul
Oncostatin M stimulates cell migration and proliferation by down-regulating E-cadherin in HTR8/SVneo cell line through STAT3 activation
title Oncostatin M stimulates cell migration and proliferation by down-regulating E-cadherin in HTR8/SVneo cell line through STAT3 activation
title_full Oncostatin M stimulates cell migration and proliferation by down-regulating E-cadherin in HTR8/SVneo cell line through STAT3 activation
title_fullStr Oncostatin M stimulates cell migration and proliferation by down-regulating E-cadherin in HTR8/SVneo cell line through STAT3 activation
title_full_unstemmed Oncostatin M stimulates cell migration and proliferation by down-regulating E-cadherin in HTR8/SVneo cell line through STAT3 activation
title_short Oncostatin M stimulates cell migration and proliferation by down-regulating E-cadherin in HTR8/SVneo cell line through STAT3 activation
title_sort oncostatin m stimulates cell migration and proliferation by down-regulating e-cadherin in htr8/svneo cell line through stat3 activation
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3849455/
https://www.ncbi.nlm.nih.gov/pubmed/24060241
http://dx.doi.org/10.1186/1477-7827-11-93
work_keys_str_mv AT kohyunsun oncostatinmstimulatescellmigrationandproliferationbydownregulatingecadherininhtr8svneocelllinethroughstat3activation
AT choisaekyung oncostatinmstimulatescellmigrationandproliferationbydownregulatingecadherininhtr8svneocelllinethroughstat3activation
AT kangheekyung oncostatinmstimulatescellmigrationandproliferationbydownregulatingecadherininhtr8svneocelllinethroughstat3activation
AT kimhoshik oncostatinmstimulatescellmigrationandproliferationbydownregulatingecadherininhtr8svneocelllinethroughstat3activation
AT jeonjihyun oncostatinmstimulatescellmigrationandproliferationbydownregulatingecadherininhtr8svneocelllinethroughstat3activation
AT parkinyang oncostatinmstimulatescellmigrationandproliferationbydownregulatingecadherininhtr8svneocelllinethroughstat3activation
AT shinjongchul oncostatinmstimulatescellmigrationandproliferationbydownregulatingecadherininhtr8svneocelllinethroughstat3activation