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Negative-pressure wound therapy induces endothelial progenitor cell mobilization in diabetic patients with foot infection or skin defects

Non healing chronic wounds are difficult to treat in patients with diabetes and can result in severe medical problems for these patients and for society. Negative-pressure wound therapy (NPWT) has been adopted to treat intractable chronic wounds and has been reported to be effective. However, the me...

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Autores principales: Seo, Sang Gyo, Yeo, Ji Hyun, Kim, JI Hye, Kim, Ji-Beom, Cho, Tae-Joon, Lee, Dong Yeon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3849576/
https://www.ncbi.nlm.nih.gov/pubmed/24232261
http://dx.doi.org/10.1038/emm.2013.129
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author Seo, Sang Gyo
Yeo, Ji Hyun
Kim, JI Hye
Kim, Ji-Beom
Cho, Tae-Joon
Lee, Dong Yeon
author_facet Seo, Sang Gyo
Yeo, Ji Hyun
Kim, JI Hye
Kim, Ji-Beom
Cho, Tae-Joon
Lee, Dong Yeon
author_sort Seo, Sang Gyo
collection PubMed
description Non healing chronic wounds are difficult to treat in patients with diabetes and can result in severe medical problems for these patients and for society. Negative-pressure wound therapy (NPWT) has been adopted to treat intractable chronic wounds and has been reported to be effective. However, the mechanisms underlying the effects of this treatment have not been elucidated. To assess the vasculogenic effect of NPWT, we evaluated the systemic mobilization of endothelial progenitor cells (EPCs) during NPWT. Twenty-two of 29 consecutive patients who presented at the clinic of Seoul National Universty Hospital between December 2009 and November 2010 who underwent NPWT for diabetic foot infections or skin ulcers were included in this study. Peripheral blood samples were taken before NPWT (pre-NPWT) and 7–14 days after the initiation of NPWT (during-NPWT). Fluorescence-activated cell sorting (FACS) analysis showed that the number of cells in EPC-enriched fractions increased after NPWT, and the numbers of EPC colony forming units (CFUs) significantly increased during NPWT. We believe that NPWT is useful for treating patients with diabetic foot infections and skin ulcers, especially when these conditions are accompanied by peripheral arterial insufficiency. The systemic mobilization of EPCs during NPWT may be a mechanism for healing intractable wounds in diabetic patients with foot infections or skin defects via the formation of increased granulation tissue with numerous small blood vessels.
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spelling pubmed-38495762013-12-06 Negative-pressure wound therapy induces endothelial progenitor cell mobilization in diabetic patients with foot infection or skin defects Seo, Sang Gyo Yeo, Ji Hyun Kim, JI Hye Kim, Ji-Beom Cho, Tae-Joon Lee, Dong Yeon Exp Mol Med Original Article Non healing chronic wounds are difficult to treat in patients with diabetes and can result in severe medical problems for these patients and for society. Negative-pressure wound therapy (NPWT) has been adopted to treat intractable chronic wounds and has been reported to be effective. However, the mechanisms underlying the effects of this treatment have not been elucidated. To assess the vasculogenic effect of NPWT, we evaluated the systemic mobilization of endothelial progenitor cells (EPCs) during NPWT. Twenty-two of 29 consecutive patients who presented at the clinic of Seoul National Universty Hospital between December 2009 and November 2010 who underwent NPWT for diabetic foot infections or skin ulcers were included in this study. Peripheral blood samples were taken before NPWT (pre-NPWT) and 7–14 days after the initiation of NPWT (during-NPWT). Fluorescence-activated cell sorting (FACS) analysis showed that the number of cells in EPC-enriched fractions increased after NPWT, and the numbers of EPC colony forming units (CFUs) significantly increased during NPWT. We believe that NPWT is useful for treating patients with diabetic foot infections and skin ulcers, especially when these conditions are accompanied by peripheral arterial insufficiency. The systemic mobilization of EPCs during NPWT may be a mechanism for healing intractable wounds in diabetic patients with foot infections or skin defects via the formation of increased granulation tissue with numerous small blood vessels. Nature Publishing Group 2013-11 2013-11-15 /pmc/articles/PMC3849576/ /pubmed/24232261 http://dx.doi.org/10.1038/emm.2013.129 Text en Copyright © 2013 KSBMB. http://creativecommons.org/licenses/by-nc-sa/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
spellingShingle Original Article
Seo, Sang Gyo
Yeo, Ji Hyun
Kim, JI Hye
Kim, Ji-Beom
Cho, Tae-Joon
Lee, Dong Yeon
Negative-pressure wound therapy induces endothelial progenitor cell mobilization in diabetic patients with foot infection or skin defects
title Negative-pressure wound therapy induces endothelial progenitor cell mobilization in diabetic patients with foot infection or skin defects
title_full Negative-pressure wound therapy induces endothelial progenitor cell mobilization in diabetic patients with foot infection or skin defects
title_fullStr Negative-pressure wound therapy induces endothelial progenitor cell mobilization in diabetic patients with foot infection or skin defects
title_full_unstemmed Negative-pressure wound therapy induces endothelial progenitor cell mobilization in diabetic patients with foot infection or skin defects
title_short Negative-pressure wound therapy induces endothelial progenitor cell mobilization in diabetic patients with foot infection or skin defects
title_sort negative-pressure wound therapy induces endothelial progenitor cell mobilization in diabetic patients with foot infection or skin defects
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3849576/
https://www.ncbi.nlm.nih.gov/pubmed/24232261
http://dx.doi.org/10.1038/emm.2013.129
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