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Expression of the gamma 2 chain of laminin-332 in eutopic and ectopic endometrium of patients with endometriosis
BACKGROUND: Endometrial cells, which are shed by retrograde menstruation, may aberrantly express molecules involved in invasion and migration, leading to endometriosis. The aim of this study was to investigate the expression of the laminin gamma 2 chain (LAMC2) in the tissues of women with and witho...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3849601/ https://www.ncbi.nlm.nih.gov/pubmed/24070183 http://dx.doi.org/10.1186/1477-7827-11-94 |
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author | Locci, Rosella Nisolle, Michelle Angioni, Stefano Foidart, Jean-Michel Munaut, Carine |
author_facet | Locci, Rosella Nisolle, Michelle Angioni, Stefano Foidart, Jean-Michel Munaut, Carine |
author_sort | Locci, Rosella |
collection | PubMed |
description | BACKGROUND: Endometrial cells, which are shed by retrograde menstruation, may aberrantly express molecules involved in invasion and migration, leading to endometriosis. The aim of this study was to investigate the expression of the laminin gamma 2 chain (LAMC2) in the tissues of women with and without endometriosis. METHODS: Endometrial biopsy specimens were collected from healthy volunteers and from endometriosis patients. Biopsy specimens from the corresponding endometriotic lesions were also collected. The expression of laminin gamma 2 chain was evaluated by immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR). RESULTS: Endometrial tissue from women with or without endometriosis showed constitutive expression of LAMC2 mRNA throughout the menstrual cycle. A higher mRNA level was observed in ectopic endometrium (Ec) from women with endometriosis compared with eutopic endometrium (Eu) from women with endometriosis. Immunohistochemistry revealed a varied pattern of laminin gamma 2 chain expression, with increased epithelial expression in eutopic endometrium from women with endometriosis compared with those without endometriosis. CONCLUSIONS: The altered expression of laminin gamma 2 chain in eutopic endometrium from women with endometriosis may provide new opportunities for diagnosis and treatment. |
format | Online Article Text |
id | pubmed-3849601 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-38496012013-12-05 Expression of the gamma 2 chain of laminin-332 in eutopic and ectopic endometrium of patients with endometriosis Locci, Rosella Nisolle, Michelle Angioni, Stefano Foidart, Jean-Michel Munaut, Carine Reprod Biol Endocrinol Research BACKGROUND: Endometrial cells, which are shed by retrograde menstruation, may aberrantly express molecules involved in invasion and migration, leading to endometriosis. The aim of this study was to investigate the expression of the laminin gamma 2 chain (LAMC2) in the tissues of women with and without endometriosis. METHODS: Endometrial biopsy specimens were collected from healthy volunteers and from endometriosis patients. Biopsy specimens from the corresponding endometriotic lesions were also collected. The expression of laminin gamma 2 chain was evaluated by immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR). RESULTS: Endometrial tissue from women with or without endometriosis showed constitutive expression of LAMC2 mRNA throughout the menstrual cycle. A higher mRNA level was observed in ectopic endometrium (Ec) from women with endometriosis compared with eutopic endometrium (Eu) from women with endometriosis. Immunohistochemistry revealed a varied pattern of laminin gamma 2 chain expression, with increased epithelial expression in eutopic endometrium from women with endometriosis compared with those without endometriosis. CONCLUSIONS: The altered expression of laminin gamma 2 chain in eutopic endometrium from women with endometriosis may provide new opportunities for diagnosis and treatment. BioMed Central 2013-09-26 /pmc/articles/PMC3849601/ /pubmed/24070183 http://dx.doi.org/10.1186/1477-7827-11-94 Text en Copyright © 2013 Locci et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Locci, Rosella Nisolle, Michelle Angioni, Stefano Foidart, Jean-Michel Munaut, Carine Expression of the gamma 2 chain of laminin-332 in eutopic and ectopic endometrium of patients with endometriosis |
title | Expression of the gamma 2 chain of laminin-332 in eutopic and ectopic endometrium of patients with endometriosis |
title_full | Expression of the gamma 2 chain of laminin-332 in eutopic and ectopic endometrium of patients with endometriosis |
title_fullStr | Expression of the gamma 2 chain of laminin-332 in eutopic and ectopic endometrium of patients with endometriosis |
title_full_unstemmed | Expression of the gamma 2 chain of laminin-332 in eutopic and ectopic endometrium of patients with endometriosis |
title_short | Expression of the gamma 2 chain of laminin-332 in eutopic and ectopic endometrium of patients with endometriosis |
title_sort | expression of the gamma 2 chain of laminin-332 in eutopic and ectopic endometrium of patients with endometriosis |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3849601/ https://www.ncbi.nlm.nih.gov/pubmed/24070183 http://dx.doi.org/10.1186/1477-7827-11-94 |
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