Cargando…

Do histopathologic findings improve by increasing the sample size in cholecystectomies?

BACKGROUND: Gallbladder diseases present with cholelithiasis in a wide spectrum of adenomas and cancers. Two or three specimens are sampled in cholecystectomies in routine pathology practice. The aim of this study was to investigate the increase in frequency of histologic alterations in cholecystect...

Descripción completa

Detalles Bibliográficos
Autores principales: Ozgur, Tumay, Toprak, Serhat, Koyuncuer, Ali, Guldur, Muhammed, Bayraktar, Gurman, Yaldiz, Mehmet
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3849800/
https://www.ncbi.nlm.nih.gov/pubmed/24261907
http://dx.doi.org/10.1186/1477-7819-11-245
_version_ 1782293991764525056
author Ozgur, Tumay
Toprak, Serhat
Koyuncuer, Ali
Guldur, Muhammed
Bayraktar, Gurman
Yaldiz, Mehmet
author_facet Ozgur, Tumay
Toprak, Serhat
Koyuncuer, Ali
Guldur, Muhammed
Bayraktar, Gurman
Yaldiz, Mehmet
author_sort Ozgur, Tumay
collection PubMed
description BACKGROUND: Gallbladder diseases present with cholelithiasis in a wide spectrum of adenomas and cancers. Two or three specimens are sampled in cholecystectomies in routine pathology practice. The aim of this study was to investigate the increase in frequency of histologic alterations in cholecystectomies, particularly precancerous lesions, by increasing the sample size to understand the carcinoma pathway. METHODS: Cholecystectomies of 432 patients with pathology records and materials from two medical centers were collected, and two groups were created. Initial data with two or three samples were allocated to Group 1 and the new six samples with the initial ones were allocated to Group 2. Hematoxylin and eosin (H&E) sections were examined for histopathologic alterations, and periodic acid–Schiff (PAS) Alcian blue (pH 2.5) and high iron diamine (pH 2.5) stains were used to signify the mucin profile in case of metaplasias. For the comparison of findings, non-parametric tests, McNemar’s tests, chi-squared tests and Fisher’s exact test were performed. RESULTS: Of the 432 patients, 308 (71.3%) patients were female and 124 (28.7%) patients were male. The mean age of patients was 47.9 ± 14.6 years. Cholesterolosis was observed in 95 (22%) patients in Group 1 and 108 (25%) patients in Group 2. Gallstones were detected in 255 (59%) of the cholecystectomies. There was a significant difference between Group 1 and Group 2 by increasing the sample size when we compared cholesterolosis, metaplasia and polyps (P <0.05). Cholecystitis and dysplasia rates were the same in both of the groups. There was no cancer determined. CONCLUSION: Increasing the sample size in cholecystectomies increased the diagnosis of some histologic alterations, but further studies with a larger number of samples over a longer period time might increase the ability to determine precancerous lesions and concomitants.
format Online
Article
Text
id pubmed-3849800
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-38498002013-12-05 Do histopathologic findings improve by increasing the sample size in cholecystectomies? Ozgur, Tumay Toprak, Serhat Koyuncuer, Ali Guldur, Muhammed Bayraktar, Gurman Yaldiz, Mehmet World J Surg Oncol Research BACKGROUND: Gallbladder diseases present with cholelithiasis in a wide spectrum of adenomas and cancers. Two or three specimens are sampled in cholecystectomies in routine pathology practice. The aim of this study was to investigate the increase in frequency of histologic alterations in cholecystectomies, particularly precancerous lesions, by increasing the sample size to understand the carcinoma pathway. METHODS: Cholecystectomies of 432 patients with pathology records and materials from two medical centers were collected, and two groups were created. Initial data with two or three samples were allocated to Group 1 and the new six samples with the initial ones were allocated to Group 2. Hematoxylin and eosin (H&E) sections were examined for histopathologic alterations, and periodic acid–Schiff (PAS) Alcian blue (pH 2.5) and high iron diamine (pH 2.5) stains were used to signify the mucin profile in case of metaplasias. For the comparison of findings, non-parametric tests, McNemar’s tests, chi-squared tests and Fisher’s exact test were performed. RESULTS: Of the 432 patients, 308 (71.3%) patients were female and 124 (28.7%) patients were male. The mean age of patients was 47.9 ± 14.6 years. Cholesterolosis was observed in 95 (22%) patients in Group 1 and 108 (25%) patients in Group 2. Gallstones were detected in 255 (59%) of the cholecystectomies. There was a significant difference between Group 1 and Group 2 by increasing the sample size when we compared cholesterolosis, metaplasia and polyps (P <0.05). Cholecystitis and dysplasia rates were the same in both of the groups. There was no cancer determined. CONCLUSION: Increasing the sample size in cholecystectomies increased the diagnosis of some histologic alterations, but further studies with a larger number of samples over a longer period time might increase the ability to determine precancerous lesions and concomitants. BioMed Central 2013-10-01 /pmc/articles/PMC3849800/ /pubmed/24261907 http://dx.doi.org/10.1186/1477-7819-11-245 Text en Copyright © 2013 Ozgur et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Ozgur, Tumay
Toprak, Serhat
Koyuncuer, Ali
Guldur, Muhammed
Bayraktar, Gurman
Yaldiz, Mehmet
Do histopathologic findings improve by increasing the sample size in cholecystectomies?
title Do histopathologic findings improve by increasing the sample size in cholecystectomies?
title_full Do histopathologic findings improve by increasing the sample size in cholecystectomies?
title_fullStr Do histopathologic findings improve by increasing the sample size in cholecystectomies?
title_full_unstemmed Do histopathologic findings improve by increasing the sample size in cholecystectomies?
title_short Do histopathologic findings improve by increasing the sample size in cholecystectomies?
title_sort do histopathologic findings improve by increasing the sample size in cholecystectomies?
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3849800/
https://www.ncbi.nlm.nih.gov/pubmed/24261907
http://dx.doi.org/10.1186/1477-7819-11-245
work_keys_str_mv AT ozgurtumay dohistopathologicfindingsimprovebyincreasingthesamplesizeincholecystectomies
AT toprakserhat dohistopathologicfindingsimprovebyincreasingthesamplesizeincholecystectomies
AT koyuncuerali dohistopathologicfindingsimprovebyincreasingthesamplesizeincholecystectomies
AT guldurmuhammed dohistopathologicfindingsimprovebyincreasingthesamplesizeincholecystectomies
AT bayraktargurman dohistopathologicfindingsimprovebyincreasingthesamplesizeincholecystectomies
AT yaldizmehmet dohistopathologicfindingsimprovebyincreasingthesamplesizeincholecystectomies