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The effect of Shenmai injection on the proliferation of Rat airway smooth muscle cells in asthma and underlying mechanism

BACKGROUND: Over-proliferation of airway smooth muscle cell (ASMC) is one of the important contributors to airway remodeling in asthma. The aim of this study was to investigate the effect of Shenmai injection (SMI) on the proliferation of the rat ASMC in asthma. METHODS: Rats were randomly divided i...

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Autores principales: Zhao, Limin, Wu, Jizhen, Zhang, Xiaoyu, Kuang, Hongyan, Guo, Yali, Ma, Lijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3849921/
https://www.ncbi.nlm.nih.gov/pubmed/24010863
http://dx.doi.org/10.1186/1472-6882-13-221
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author Zhao, Limin
Wu, Jizhen
Zhang, Xiaoyu
Kuang, Hongyan
Guo, Yali
Ma, Lijun
author_facet Zhao, Limin
Wu, Jizhen
Zhang, Xiaoyu
Kuang, Hongyan
Guo, Yali
Ma, Lijun
author_sort Zhao, Limin
collection PubMed
description BACKGROUND: Over-proliferation of airway smooth muscle cell (ASMC) is one of the important contributors to airway remodeling in asthma. The aim of this study was to investigate the effect of Shenmai injection (SMI) on the proliferation of the rat ASMC in asthma. METHODS: Rats were randomly divided into three groups: the control group, the asthma group, and the SMI treatment group. Reverse transcription-polymerase chain reaction (RT-PCR) and immunocytochemistry staining were used to detect the mRNA and protein expression of transient receptor potential vanilloid 1 (TRPV1) and proliferating cell nuclear antigen (PCNA) in rat ASMC respectively. Intracellular Ca(2+) concentration ( [Ca(2+)]i ) in rat ASMC were measured with Fluo-3/AM by confocal microscopy. The proliferation was detected by MTT assay. RESULTS: Compared with the control group, the asthma group showed an increased expression of TRPV1 and [Ca(2+)]i in rat ASMC. The expression of PCNA and absorbance of MTT assay in asthma rat ASMC was also significantly increased. SMI could significantly decrease the expression of TRPV1 channel and [Ca(2+)]i in the asthmatic rat ASMC. Furthermore, the expression of PCNA and absorbance of MTT assay in asthmatic rat ASMC was significantly reduced after SMI treatment. CONCLUSIONS: SMI may prevent asthma-induced ASMC over-proliferation probably by inhibiting the expression of TRPV1 channel, which regulates the intracellular calcium concentration.
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spelling pubmed-38499212013-12-18 The effect of Shenmai injection on the proliferation of Rat airway smooth muscle cells in asthma and underlying mechanism Zhao, Limin Wu, Jizhen Zhang, Xiaoyu Kuang, Hongyan Guo, Yali Ma, Lijun BMC Complement Altern Med Research Article BACKGROUND: Over-proliferation of airway smooth muscle cell (ASMC) is one of the important contributors to airway remodeling in asthma. The aim of this study was to investigate the effect of Shenmai injection (SMI) on the proliferation of the rat ASMC in asthma. METHODS: Rats were randomly divided into three groups: the control group, the asthma group, and the SMI treatment group. Reverse transcription-polymerase chain reaction (RT-PCR) and immunocytochemistry staining were used to detect the mRNA and protein expression of transient receptor potential vanilloid 1 (TRPV1) and proliferating cell nuclear antigen (PCNA) in rat ASMC respectively. Intracellular Ca(2+) concentration ( [Ca(2+)]i ) in rat ASMC were measured with Fluo-3/AM by confocal microscopy. The proliferation was detected by MTT assay. RESULTS: Compared with the control group, the asthma group showed an increased expression of TRPV1 and [Ca(2+)]i in rat ASMC. The expression of PCNA and absorbance of MTT assay in asthma rat ASMC was also significantly increased. SMI could significantly decrease the expression of TRPV1 channel and [Ca(2+)]i in the asthmatic rat ASMC. Furthermore, the expression of PCNA and absorbance of MTT assay in asthmatic rat ASMC was significantly reduced after SMI treatment. CONCLUSIONS: SMI may prevent asthma-induced ASMC over-proliferation probably by inhibiting the expression of TRPV1 channel, which regulates the intracellular calcium concentration. BioMed Central 2013-09-08 /pmc/articles/PMC3849921/ /pubmed/24010863 http://dx.doi.org/10.1186/1472-6882-13-221 Text en Copyright © 2013 Zhao et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhao, Limin
Wu, Jizhen
Zhang, Xiaoyu
Kuang, Hongyan
Guo, Yali
Ma, Lijun
The effect of Shenmai injection on the proliferation of Rat airway smooth muscle cells in asthma and underlying mechanism
title The effect of Shenmai injection on the proliferation of Rat airway smooth muscle cells in asthma and underlying mechanism
title_full The effect of Shenmai injection on the proliferation of Rat airway smooth muscle cells in asthma and underlying mechanism
title_fullStr The effect of Shenmai injection on the proliferation of Rat airway smooth muscle cells in asthma and underlying mechanism
title_full_unstemmed The effect of Shenmai injection on the proliferation of Rat airway smooth muscle cells in asthma and underlying mechanism
title_short The effect of Shenmai injection on the proliferation of Rat airway smooth muscle cells in asthma and underlying mechanism
title_sort effect of shenmai injection on the proliferation of rat airway smooth muscle cells in asthma and underlying mechanism
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3849921/
https://www.ncbi.nlm.nih.gov/pubmed/24010863
http://dx.doi.org/10.1186/1472-6882-13-221
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