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The Developmental Competence of Oocytes Retrieved from The Leading Follicle in Controlled Ovarian Stimulated Cycles
BACKGROUND: This study compares the developmental capacity of gametes retrieved from the largest follicle with small follicles of a cohort in controlled ovarian stimulated cycles. MATERIALS AND METHODS: This prospective study performed in a private assisted fertilization center included 1016 follicl...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royan Institute
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3850321/ https://www.ncbi.nlm.nih.gov/pubmed/24520451 |
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author | Braga, Daniela Paes de Almeida Ferreira Bonetti, Tatiana Carvalho de Souza da Silva, Ismael Dale Cotrim Guerreiro Setti, Amanda Souza Iaconelli, Assumpto Jr Borges, Edson Jr |
author_facet | Braga, Daniela Paes de Almeida Ferreira Bonetti, Tatiana Carvalho de Souza da Silva, Ismael Dale Cotrim Guerreiro Setti, Amanda Souza Iaconelli, Assumpto Jr Borges, Edson Jr |
author_sort | Braga, Daniela Paes de Almeida Ferreira |
collection | PubMed |
description | BACKGROUND: This study compares the developmental capacity of gametes retrieved from the largest follicle with small follicles of a cohort in controlled ovarian stimulated cycles. MATERIALS AND METHODS: This prospective study performed in a private assisted fertilization center included 1016 follicles collected from 96 patients who underwent intra cytoplasmic sperm injection (ICSI). After follicular aspiration, oocytes were assigned to two groups according to the diameter of the derived follicle. The large follicle group (n=96) comprised oocytes derived from the leading follicle of the cohort and the small follicle group (n=920) consisted oocytes derived from the smaller follicles of the cohort. The fertilization and percentage of topquality embryos were compared between groups by Chi-square or Fisher’s exact test, where appropriate. The effect of the follicular diameter on oocyte dimorphism was assessed by binary logistic regression. RESULTS: A significantly higher percentage of oocytes derived from the leading follicle were in the metaphase II (MII) stage (100 vs. 70.0%, p<0.001). However we observed no significant differences regarding the percentage of degenerated oocytes between the large (6.25%) and small follicle (5.0%) groups (p=0.550). Regression analysis demonstrated a nearly two-fold increase in the incidence of vacuoles in oocytes derived from the largest follicle of the cohort (OR: 1.81, p=0.046). The fertilization rate (50.0 vs. 38.8%, p=0.038) and the percentage of top quality embryos (84.7 vs. 76.4%, p=0.040) were significantly higher for oocytes derived from the largest follicle. However, the percentage of abnormal fertilized oocytes was equally distributed between the large follicle (15.0%) and small follicle (12. 8%) groups (p=0.550). CONCLUSION: Our data suggest that intrafollicular mechanisms within the larger follicle of the cohort may allow for these follicles to amplify the responsiveness to exogenous gonadotropin, which leads to the formation of more competent oocytes with higher fertilization and developmental capacities. |
format | Online Article Text |
id | pubmed-3850321 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Royan Institute |
record_format | MEDLINE/PubMed |
spelling | pubmed-38503212014-02-11 The Developmental Competence of Oocytes Retrieved from The Leading Follicle in Controlled Ovarian Stimulated Cycles Braga, Daniela Paes de Almeida Ferreira Bonetti, Tatiana Carvalho de Souza da Silva, Ismael Dale Cotrim Guerreiro Setti, Amanda Souza Iaconelli, Assumpto Jr Borges, Edson Jr Int J Fertil Steril Original Article BACKGROUND: This study compares the developmental capacity of gametes retrieved from the largest follicle with small follicles of a cohort in controlled ovarian stimulated cycles. MATERIALS AND METHODS: This prospective study performed in a private assisted fertilization center included 1016 follicles collected from 96 patients who underwent intra cytoplasmic sperm injection (ICSI). After follicular aspiration, oocytes were assigned to two groups according to the diameter of the derived follicle. The large follicle group (n=96) comprised oocytes derived from the leading follicle of the cohort and the small follicle group (n=920) consisted oocytes derived from the smaller follicles of the cohort. The fertilization and percentage of topquality embryos were compared between groups by Chi-square or Fisher’s exact test, where appropriate. The effect of the follicular diameter on oocyte dimorphism was assessed by binary logistic regression. RESULTS: A significantly higher percentage of oocytes derived from the leading follicle were in the metaphase II (MII) stage (100 vs. 70.0%, p<0.001). However we observed no significant differences regarding the percentage of degenerated oocytes between the large (6.25%) and small follicle (5.0%) groups (p=0.550). Regression analysis demonstrated a nearly two-fold increase in the incidence of vacuoles in oocytes derived from the largest follicle of the cohort (OR: 1.81, p=0.046). The fertilization rate (50.0 vs. 38.8%, p=0.038) and the percentage of top quality embryos (84.7 vs. 76.4%, p=0.040) were significantly higher for oocytes derived from the largest follicle. However, the percentage of abnormal fertilized oocytes was equally distributed between the large follicle (15.0%) and small follicle (12. 8%) groups (p=0.550). CONCLUSION: Our data suggest that intrafollicular mechanisms within the larger follicle of the cohort may allow for these follicles to amplify the responsiveness to exogenous gonadotropin, which leads to the formation of more competent oocytes with higher fertilization and developmental capacities. Royan Institute 2013 2013-03-03 /pmc/articles/PMC3850321/ /pubmed/24520451 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Braga, Daniela Paes de Almeida Ferreira Bonetti, Tatiana Carvalho de Souza da Silva, Ismael Dale Cotrim Guerreiro Setti, Amanda Souza Iaconelli, Assumpto Jr Borges, Edson Jr The Developmental Competence of Oocytes Retrieved from The Leading Follicle in Controlled Ovarian Stimulated Cycles |
title | The Developmental Competence of Oocytes Retrieved from The
Leading Follicle in Controlled Ovarian Stimulated Cycles |
title_full | The Developmental Competence of Oocytes Retrieved from The
Leading Follicle in Controlled Ovarian Stimulated Cycles |
title_fullStr | The Developmental Competence of Oocytes Retrieved from The
Leading Follicle in Controlled Ovarian Stimulated Cycles |
title_full_unstemmed | The Developmental Competence of Oocytes Retrieved from The
Leading Follicle in Controlled Ovarian Stimulated Cycles |
title_short | The Developmental Competence of Oocytes Retrieved from The
Leading Follicle in Controlled Ovarian Stimulated Cycles |
title_sort | developmental competence of oocytes retrieved from the
leading follicle in controlled ovarian stimulated cycles |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3850321/ https://www.ncbi.nlm.nih.gov/pubmed/24520451 |
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