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The Developmental Competence of Oocytes Retrieved from The Leading Follicle in Controlled Ovarian Stimulated Cycles

BACKGROUND: This study compares the developmental capacity of gametes retrieved from the largest follicle with small follicles of a cohort in controlled ovarian stimulated cycles. MATERIALS AND METHODS: This prospective study performed in a private assisted fertilization center included 1016 follicl...

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Autores principales: Braga, Daniela Paes de Almeida Ferreira, Bonetti, Tatiana Carvalho de Souza, da Silva, Ismael Dale Cotrim Guerreiro, Setti, Amanda Souza, Iaconelli, Assumpto Jr, Borges, Edson Jr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royan Institute 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3850321/
https://www.ncbi.nlm.nih.gov/pubmed/24520451
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author Braga, Daniela Paes de Almeida Ferreira
Bonetti, Tatiana Carvalho de Souza
da Silva, Ismael Dale Cotrim Guerreiro
Setti, Amanda Souza
Iaconelli, Assumpto Jr
Borges, Edson Jr
author_facet Braga, Daniela Paes de Almeida Ferreira
Bonetti, Tatiana Carvalho de Souza
da Silva, Ismael Dale Cotrim Guerreiro
Setti, Amanda Souza
Iaconelli, Assumpto Jr
Borges, Edson Jr
author_sort Braga, Daniela Paes de Almeida Ferreira
collection PubMed
description BACKGROUND: This study compares the developmental capacity of gametes retrieved from the largest follicle with small follicles of a cohort in controlled ovarian stimulated cycles. MATERIALS AND METHODS: This prospective study performed in a private assisted fertilization center included 1016 follicles collected from 96 patients who underwent intra cytoplasmic sperm injection (ICSI). After follicular aspiration, oocytes were assigned to two groups according to the diameter of the derived follicle. The large follicle group (n=96) comprised oocytes derived from the leading follicle of the cohort and the small follicle group (n=920) consisted oocytes derived from the smaller follicles of the cohort. The fertilization and percentage of topquality embryos were compared between groups by Chi-square or Fisher’s exact test, where appropriate. The effect of the follicular diameter on oocyte dimorphism was assessed by binary logistic regression. RESULTS: A significantly higher percentage of oocytes derived from the leading follicle were in the metaphase II (MII) stage (100 vs. 70.0%, p<0.001). However we observed no significant differences regarding the percentage of degenerated oocytes between the large (6.25%) and small follicle (5.0%) groups (p=0.550). Regression analysis demonstrated a nearly two-fold increase in the incidence of vacuoles in oocytes derived from the largest follicle of the cohort (OR: 1.81, p=0.046). The fertilization rate (50.0 vs. 38.8%, p=0.038) and the percentage of top quality embryos (84.7 vs. 76.4%, p=0.040) were significantly higher for oocytes derived from the largest follicle. However, the percentage of abnormal fertilized oocytes was equally distributed between the large follicle (15.0%) and small follicle (12. 8%) groups (p=0.550). CONCLUSION: Our data suggest that intrafollicular mechanisms within the larger follicle of the cohort may allow for these follicles to amplify the responsiveness to exogenous gonadotropin, which leads to the formation of more competent oocytes with higher fertilization and developmental capacities.
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spelling pubmed-38503212014-02-11 The Developmental Competence of Oocytes Retrieved from The Leading Follicle in Controlled Ovarian Stimulated Cycles Braga, Daniela Paes de Almeida Ferreira Bonetti, Tatiana Carvalho de Souza da Silva, Ismael Dale Cotrim Guerreiro Setti, Amanda Souza Iaconelli, Assumpto Jr Borges, Edson Jr Int J Fertil Steril Original Article BACKGROUND: This study compares the developmental capacity of gametes retrieved from the largest follicle with small follicles of a cohort in controlled ovarian stimulated cycles. MATERIALS AND METHODS: This prospective study performed in a private assisted fertilization center included 1016 follicles collected from 96 patients who underwent intra cytoplasmic sperm injection (ICSI). After follicular aspiration, oocytes were assigned to two groups according to the diameter of the derived follicle. The large follicle group (n=96) comprised oocytes derived from the leading follicle of the cohort and the small follicle group (n=920) consisted oocytes derived from the smaller follicles of the cohort. The fertilization and percentage of topquality embryos were compared between groups by Chi-square or Fisher’s exact test, where appropriate. The effect of the follicular diameter on oocyte dimorphism was assessed by binary logistic regression. RESULTS: A significantly higher percentage of oocytes derived from the leading follicle were in the metaphase II (MII) stage (100 vs. 70.0%, p<0.001). However we observed no significant differences regarding the percentage of degenerated oocytes between the large (6.25%) and small follicle (5.0%) groups (p=0.550). Regression analysis demonstrated a nearly two-fold increase in the incidence of vacuoles in oocytes derived from the largest follicle of the cohort (OR: 1.81, p=0.046). The fertilization rate (50.0 vs. 38.8%, p=0.038) and the percentage of top quality embryos (84.7 vs. 76.4%, p=0.040) were significantly higher for oocytes derived from the largest follicle. However, the percentage of abnormal fertilized oocytes was equally distributed between the large follicle (15.0%) and small follicle (12. 8%) groups (p=0.550). CONCLUSION: Our data suggest that intrafollicular mechanisms within the larger follicle of the cohort may allow for these follicles to amplify the responsiveness to exogenous gonadotropin, which leads to the formation of more competent oocytes with higher fertilization and developmental capacities. Royan Institute 2013 2013-03-03 /pmc/articles/PMC3850321/ /pubmed/24520451 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Braga, Daniela Paes de Almeida Ferreira
Bonetti, Tatiana Carvalho de Souza
da Silva, Ismael Dale Cotrim Guerreiro
Setti, Amanda Souza
Iaconelli, Assumpto Jr
Borges, Edson Jr
The Developmental Competence of Oocytes Retrieved from The Leading Follicle in Controlled Ovarian Stimulated Cycles
title The Developmental Competence of Oocytes Retrieved from The Leading Follicle in Controlled Ovarian Stimulated Cycles
title_full The Developmental Competence of Oocytes Retrieved from The Leading Follicle in Controlled Ovarian Stimulated Cycles
title_fullStr The Developmental Competence of Oocytes Retrieved from The Leading Follicle in Controlled Ovarian Stimulated Cycles
title_full_unstemmed The Developmental Competence of Oocytes Retrieved from The Leading Follicle in Controlled Ovarian Stimulated Cycles
title_short The Developmental Competence of Oocytes Retrieved from The Leading Follicle in Controlled Ovarian Stimulated Cycles
title_sort developmental competence of oocytes retrieved from the leading follicle in controlled ovarian stimulated cycles
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3850321/
https://www.ncbi.nlm.nih.gov/pubmed/24520451
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