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Elevation in Peripheral Blood Circulating Tumor Cell Number Correlates with Macroscopic Progression in UICC Stage IV Colorectal Cancer Patients

Aims: Cytokeratin(CK) based real-time RT-PCR assays (QRT-PCR) are now available for peripheral blood circulating tumor cell (CTC) evaluations in colorectal cancer(CRC) patients. Results are non-existent for the application of these techniques to the determination of progression and therapy response...

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Autores principales: Molnar, Bela, Floro, Lajos, Sipos, Ferenc, Toth, Bernadett, Sreter, Lydia, Tulassay, Zsolt
Formato: Online Artículo Texto
Lenguaje:English
Publicado: IOS Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3850608/
https://www.ncbi.nlm.nih.gov/pubmed/18334735
http://dx.doi.org/10.1155/2008/941509
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author Molnar, Bela
Floro, Lajos
Sipos, Ferenc
Toth, Bernadett
Sreter, Lydia
Tulassay, Zsolt
author_facet Molnar, Bela
Floro, Lajos
Sipos, Ferenc
Toth, Bernadett
Sreter, Lydia
Tulassay, Zsolt
author_sort Molnar, Bela
collection PubMed
description Aims: Cytokeratin(CK) based real-time RT-PCR assays (QRT-PCR) are now available for peripheral blood circulating tumor cell (CTC) evaluations in colorectal cancer(CRC) patients. Results are non-existent for the application of these techniques to the determination of progression and therapy response in Dukes stage D CRC patients. Patients and methods: Each month 30 ml peripheral blood of 30 Dukes D patients (17 with progression) were drawn. CEA, CA19-9, CA72-A and TPA-M determinations were made. CK20, thymidilate synthase(TS) QRT-PCR was performed, as well. Buffy coat was used for immunmagnetic cancer cell isolation and CTC counting. Correlation between elevated CTC and macroscopic progression within 3 months was determined by Chi(2) test. Results: Microscopic CTC single cell, doublet, cluster number were found in correlation with CK20 QRT-PCR results (p < 0.01). There was a significant increase in microscopic CTC number, CK20 and decrease in TS QRT-PCR levels (p < 0.05) in the peripheral blood of the non-responder as compared to responder patients. Elevation of the CTC was in significant correlation with macroscopic progression of the disease in 3 months (p < 0.01). Conclusions: CTC number reflects the chemotherapeutic sensitivity of CRC patients. Elevation of circulating tumor cell number in peripheral blood is in correlation with macroscopic progression.
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spelling pubmed-38506082013-12-12 Elevation in Peripheral Blood Circulating Tumor Cell Number Correlates with Macroscopic Progression in UICC Stage IV Colorectal Cancer Patients Molnar, Bela Floro, Lajos Sipos, Ferenc Toth, Bernadett Sreter, Lydia Tulassay, Zsolt Dis Markers Other Aims: Cytokeratin(CK) based real-time RT-PCR assays (QRT-PCR) are now available for peripheral blood circulating tumor cell (CTC) evaluations in colorectal cancer(CRC) patients. Results are non-existent for the application of these techniques to the determination of progression and therapy response in Dukes stage D CRC patients. Patients and methods: Each month 30 ml peripheral blood of 30 Dukes D patients (17 with progression) were drawn. CEA, CA19-9, CA72-A and TPA-M determinations were made. CK20, thymidilate synthase(TS) QRT-PCR was performed, as well. Buffy coat was used for immunmagnetic cancer cell isolation and CTC counting. Correlation between elevated CTC and macroscopic progression within 3 months was determined by Chi(2) test. Results: Microscopic CTC single cell, doublet, cluster number were found in correlation with CK20 QRT-PCR results (p < 0.01). There was a significant increase in microscopic CTC number, CK20 and decrease in TS QRT-PCR levels (p < 0.05) in the peripheral blood of the non-responder as compared to responder patients. Elevation of the CTC was in significant correlation with macroscopic progression of the disease in 3 months (p < 0.01). Conclusions: CTC number reflects the chemotherapeutic sensitivity of CRC patients. Elevation of circulating tumor cell number in peripheral blood is in correlation with macroscopic progression. IOS Press 2008 2008-03-04 /pmc/articles/PMC3850608/ /pubmed/18334735 http://dx.doi.org/10.1155/2008/941509 Text en Copyright © 2008 Hindawi Publishing Corporation.
spellingShingle Other
Molnar, Bela
Floro, Lajos
Sipos, Ferenc
Toth, Bernadett
Sreter, Lydia
Tulassay, Zsolt
Elevation in Peripheral Blood Circulating Tumor Cell Number Correlates with Macroscopic Progression in UICC Stage IV Colorectal Cancer Patients
title Elevation in Peripheral Blood Circulating Tumor Cell Number Correlates with Macroscopic Progression in UICC Stage IV Colorectal Cancer Patients
title_full Elevation in Peripheral Blood Circulating Tumor Cell Number Correlates with Macroscopic Progression in UICC Stage IV Colorectal Cancer Patients
title_fullStr Elevation in Peripheral Blood Circulating Tumor Cell Number Correlates with Macroscopic Progression in UICC Stage IV Colorectal Cancer Patients
title_full_unstemmed Elevation in Peripheral Blood Circulating Tumor Cell Number Correlates with Macroscopic Progression in UICC Stage IV Colorectal Cancer Patients
title_short Elevation in Peripheral Blood Circulating Tumor Cell Number Correlates with Macroscopic Progression in UICC Stage IV Colorectal Cancer Patients
title_sort elevation in peripheral blood circulating tumor cell number correlates with macroscopic progression in uicc stage iv colorectal cancer patients
topic Other
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3850608/
https://www.ncbi.nlm.nih.gov/pubmed/18334735
http://dx.doi.org/10.1155/2008/941509
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