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Synaptophysin is involved in resetting of the mammalian circadian clock

BACKGROUND: Mammals can adapt to changing light/dark conditions by advancing or delaying their circadian clock phase. Light pulses evoke changes in gene expression and neuronal activity in the suprachiasmatic nuclei (SCN), the central pacemaker of the circadian system. Alterations in neuronal activi...

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Autores principales: Aramendy, Marie, Seibert, Sascha, Treppmann, Philipp, Richter, Karin, Ahnert-Hilger, Gudrun, Albrecht, Urs
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3851196/
https://www.ncbi.nlm.nih.gov/pubmed/24083423
http://dx.doi.org/10.1186/1740-3391-11-11
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author Aramendy, Marie
Seibert, Sascha
Treppmann, Philipp
Richter, Karin
Ahnert-Hilger, Gudrun
Albrecht, Urs
author_facet Aramendy, Marie
Seibert, Sascha
Treppmann, Philipp
Richter, Karin
Ahnert-Hilger, Gudrun
Albrecht, Urs
author_sort Aramendy, Marie
collection PubMed
description BACKGROUND: Mammals can adapt to changing light/dark conditions by advancing or delaying their circadian clock phase. Light pulses evoke changes in gene expression and neuronal activity in the suprachiasmatic nuclei (SCN), the central pacemaker of the circadian system. Alterations in neuronal activity are partially mediated by changes in synaptic vesicle (SV) fusion at the presynaptic membrane, which modulates release of neurotransmitters. METHODS: Male synaptophysin (Syp) knock-out and littermate control wild type mice were tested in an Aschoff type I resetting paradigm. Additionally, gene expression of cFos, Per1 and Per2 was assessed in the SCN. Finally, complexes between the synaptic vesicle proteins Syp and synaptobrevin (Syb) were studied in order to correlate behavior with protein complexes at synaptic vesicles. RESULTS: Here we show that mice lacking Syp, a modulator of neurotransmitter release, are defective in delaying clock phase. In contrast, clock phase advances as well as clock period are normal in Syp(-/-) knock-out mice. This correlates with the formation of Syp/Syb complexes. CONCLUSIONS: Our findings suggest that Syp is involved specifically in the response to a nocturnal light pulse occurring in the early night. It appears that the SV component Syp is critically involved in the delay portion of the resetting mechanism of the circadian clock.
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spelling pubmed-38511962013-12-06 Synaptophysin is involved in resetting of the mammalian circadian clock Aramendy, Marie Seibert, Sascha Treppmann, Philipp Richter, Karin Ahnert-Hilger, Gudrun Albrecht, Urs J Circadian Rhythms Research BACKGROUND: Mammals can adapt to changing light/dark conditions by advancing or delaying their circadian clock phase. Light pulses evoke changes in gene expression and neuronal activity in the suprachiasmatic nuclei (SCN), the central pacemaker of the circadian system. Alterations in neuronal activity are partially mediated by changes in synaptic vesicle (SV) fusion at the presynaptic membrane, which modulates release of neurotransmitters. METHODS: Male synaptophysin (Syp) knock-out and littermate control wild type mice were tested in an Aschoff type I resetting paradigm. Additionally, gene expression of cFos, Per1 and Per2 was assessed in the SCN. Finally, complexes between the synaptic vesicle proteins Syp and synaptobrevin (Syb) were studied in order to correlate behavior with protein complexes at synaptic vesicles. RESULTS: Here we show that mice lacking Syp, a modulator of neurotransmitter release, are defective in delaying clock phase. In contrast, clock phase advances as well as clock period are normal in Syp(-/-) knock-out mice. This correlates with the formation of Syp/Syb complexes. CONCLUSIONS: Our findings suggest that Syp is involved specifically in the response to a nocturnal light pulse occurring in the early night. It appears that the SV component Syp is critically involved in the delay portion of the resetting mechanism of the circadian clock. BioMed Central 2013-10-01 /pmc/articles/PMC3851196/ /pubmed/24083423 http://dx.doi.org/10.1186/1740-3391-11-11 Text en Copyright © 2013 Aramendy et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Aramendy, Marie
Seibert, Sascha
Treppmann, Philipp
Richter, Karin
Ahnert-Hilger, Gudrun
Albrecht, Urs
Synaptophysin is involved in resetting of the mammalian circadian clock
title Synaptophysin is involved in resetting of the mammalian circadian clock
title_full Synaptophysin is involved in resetting of the mammalian circadian clock
title_fullStr Synaptophysin is involved in resetting of the mammalian circadian clock
title_full_unstemmed Synaptophysin is involved in resetting of the mammalian circadian clock
title_short Synaptophysin is involved in resetting of the mammalian circadian clock
title_sort synaptophysin is involved in resetting of the mammalian circadian clock
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3851196/
https://www.ncbi.nlm.nih.gov/pubmed/24083423
http://dx.doi.org/10.1186/1740-3391-11-11
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