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Lower muscle regenerative potential in full-thickness supraspinatus tears compared to partial-thickness tears

BACKGROUND AND PURPOSE: Rotator cuff tears are associated with secondary rotator cuff muscle pathology, which is definitive for the prognosis of rotator cuff repair. There is little information regarding the early histological and immunohistochemical nature of these muscle changes in humans. We anal...

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Autores principales: Lundgreen, Kirsten, Lian, Øystein Bjerkestrand, Engebretsen, Lars, Scott, Alex
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Informa Healthcare 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3851671/
https://www.ncbi.nlm.nih.gov/pubmed/24171689
http://dx.doi.org/10.3109/17453674.2013.858289
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author Lundgreen, Kirsten
Lian, Øystein Bjerkestrand
Engebretsen, Lars
Scott, Alex
author_facet Lundgreen, Kirsten
Lian, Øystein Bjerkestrand
Engebretsen, Lars
Scott, Alex
author_sort Lundgreen, Kirsten
collection PubMed
description BACKGROUND AND PURPOSE: Rotator cuff tears are associated with secondary rotator cuff muscle pathology, which is definitive for the prognosis of rotator cuff repair. There is little information regarding the early histological and immunohistochemical nature of these muscle changes in humans. We analyzed muscle biopsies from patients with supraspinatus tendon tears. METHODS: Supraspinatus muscle biopsies were obtained from 24 patients undergoing arthroscopic repair of partial- or full-thickness supraspinatus tendon tears. Tissue was formalin-fixed and processed for histology (for assessment of fatty infiltration and other degenerative changes) or immunohistochemistry (to identify satellite cells (CD56+), proliferating cells (Ki67+), and myofibers containing predominantly type 1 or 2 myosin heavy chain (MHC)). Myofiber diameters and the relative content of MHC1 and MHC2 were determined morphometrically. RESULTS: Degenerative changes were present in both patient groups (partial and full-thickness tears). Patients with full-thickness tears had a reduced density of satellite cells, fewer proliferating cells, atrophy of MHC1+ and MHC2+ myofibers, and reduced MHC1 content. INTERPRETATION: Full-thickness tears show significantly reduced muscle proliferative capacity, myofiber atrophy, and loss of MHC1 content compared to partial-thickness supraspinatus tendon tears.
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spelling pubmed-38516712013-12-08 Lower muscle regenerative potential in full-thickness supraspinatus tears compared to partial-thickness tears Lundgreen, Kirsten Lian, Øystein Bjerkestrand Engebretsen, Lars Scott, Alex Acta Orthop Article BACKGROUND AND PURPOSE: Rotator cuff tears are associated with secondary rotator cuff muscle pathology, which is definitive for the prognosis of rotator cuff repair. There is little information regarding the early histological and immunohistochemical nature of these muscle changes in humans. We analyzed muscle biopsies from patients with supraspinatus tendon tears. METHODS: Supraspinatus muscle biopsies were obtained from 24 patients undergoing arthroscopic repair of partial- or full-thickness supraspinatus tendon tears. Tissue was formalin-fixed and processed for histology (for assessment of fatty infiltration and other degenerative changes) or immunohistochemistry (to identify satellite cells (CD56+), proliferating cells (Ki67+), and myofibers containing predominantly type 1 or 2 myosin heavy chain (MHC)). Myofiber diameters and the relative content of MHC1 and MHC2 were determined morphometrically. RESULTS: Degenerative changes were present in both patient groups (partial and full-thickness tears). Patients with full-thickness tears had a reduced density of satellite cells, fewer proliferating cells, atrophy of MHC1+ and MHC2+ myofibers, and reduced MHC1 content. INTERPRETATION: Full-thickness tears show significantly reduced muscle proliferative capacity, myofiber atrophy, and loss of MHC1 content compared to partial-thickness supraspinatus tendon tears. Informa Healthcare 2013-12 2013-12-02 /pmc/articles/PMC3851671/ /pubmed/24171689 http://dx.doi.org/10.3109/17453674.2013.858289 Text en Copyright: © Nordic Orthopaedic Federation http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the source is credited.
spellingShingle Article
Lundgreen, Kirsten
Lian, Øystein Bjerkestrand
Engebretsen, Lars
Scott, Alex
Lower muscle regenerative potential in full-thickness supraspinatus tears compared to partial-thickness tears
title Lower muscle regenerative potential in full-thickness supraspinatus tears compared to partial-thickness tears
title_full Lower muscle regenerative potential in full-thickness supraspinatus tears compared to partial-thickness tears
title_fullStr Lower muscle regenerative potential in full-thickness supraspinatus tears compared to partial-thickness tears
title_full_unstemmed Lower muscle regenerative potential in full-thickness supraspinatus tears compared to partial-thickness tears
title_short Lower muscle regenerative potential in full-thickness supraspinatus tears compared to partial-thickness tears
title_sort lower muscle regenerative potential in full-thickness supraspinatus tears compared to partial-thickness tears
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3851671/
https://www.ncbi.nlm.nih.gov/pubmed/24171689
http://dx.doi.org/10.3109/17453674.2013.858289
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