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Gene expression of sternohyoid and diaphragm muscles in type 2 diabetic rats
BACKGROUND: Type 2 diabetes differs from type 1 diabetes in its pathogenesis. Type 1 diabetic diaphragm has altered gene expression which includes lipid and carbohydrate metabolism, ubiquitination and oxidoreductase activity. The objectives of the present study were to assess respiratory muscle gene...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3851765/ https://www.ncbi.nlm.nih.gov/pubmed/24199937 http://dx.doi.org/10.1186/1472-6823-13-43 |
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author | van Lunteren, Erik Moyer, Michelle |
author_facet | van Lunteren, Erik Moyer, Michelle |
author_sort | van Lunteren, Erik |
collection | PubMed |
description | BACKGROUND: Type 2 diabetes differs from type 1 diabetes in its pathogenesis. Type 1 diabetic diaphragm has altered gene expression which includes lipid and carbohydrate metabolism, ubiquitination and oxidoreductase activity. The objectives of the present study were to assess respiratory muscle gene expression changes in type 2 diabetes and to determine whether they are greater for the diaphragm than an upper airway muscle. METHODS: Diaphragm and sternohyoid muscle from Zucker diabetic fatty (ZDF) rats were analyzed with Affymetrix gene expression arrays. RESULTS: The two muscles had 97 and 102 genes, respectively, with at least ± 1.5-fold significantly changed expression with diabetes, and these were assigned to gene ontology groups based on over-representation analysis. Several significantly changed groups were common to both muscles, including lipid metabolism, carbohydrate metabolism, muscle contraction, ion transport and collagen, although the number of genes and the specific genes involved differed considerably for the two muscles. In both muscles there was a shift in metabolism gene expression from carbohydrate metabolism toward lipid metabolism, but the shift was greater and involved more genes in diabetic diaphragm than diabetic sternohyoid muscle. Groups present in only diaphragm were blood circulation and oxidoreductase activity. Groups present in only sternohyoid were immune & inflammation and response to stress & wounding, with complement genes being a prominent component. CONCLUSION: Type 2 diabetes-induced gene expression changes in respiratory muscles has both similarities and differences relative to previous data on type 1 diabetes gene expression. Furthermore, the diabetic alterations in gene expression differ between diaphragm and sternohyoid. |
format | Online Article Text |
id | pubmed-3851765 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-38517652013-12-06 Gene expression of sternohyoid and diaphragm muscles in type 2 diabetic rats van Lunteren, Erik Moyer, Michelle BMC Endocr Disord Research Article BACKGROUND: Type 2 diabetes differs from type 1 diabetes in its pathogenesis. Type 1 diabetic diaphragm has altered gene expression which includes lipid and carbohydrate metabolism, ubiquitination and oxidoreductase activity. The objectives of the present study were to assess respiratory muscle gene expression changes in type 2 diabetes and to determine whether they are greater for the diaphragm than an upper airway muscle. METHODS: Diaphragm and sternohyoid muscle from Zucker diabetic fatty (ZDF) rats were analyzed with Affymetrix gene expression arrays. RESULTS: The two muscles had 97 and 102 genes, respectively, with at least ± 1.5-fold significantly changed expression with diabetes, and these were assigned to gene ontology groups based on over-representation analysis. Several significantly changed groups were common to both muscles, including lipid metabolism, carbohydrate metabolism, muscle contraction, ion transport and collagen, although the number of genes and the specific genes involved differed considerably for the two muscles. In both muscles there was a shift in metabolism gene expression from carbohydrate metabolism toward lipid metabolism, but the shift was greater and involved more genes in diabetic diaphragm than diabetic sternohyoid muscle. Groups present in only diaphragm were blood circulation and oxidoreductase activity. Groups present in only sternohyoid were immune & inflammation and response to stress & wounding, with complement genes being a prominent component. CONCLUSION: Type 2 diabetes-induced gene expression changes in respiratory muscles has both similarities and differences relative to previous data on type 1 diabetes gene expression. Furthermore, the diabetic alterations in gene expression differ between diaphragm and sternohyoid. BioMed Central 2013-10-07 /pmc/articles/PMC3851765/ /pubmed/24199937 http://dx.doi.org/10.1186/1472-6823-13-43 Text en Copyright © 2013 van Lunteren and Moyer; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article van Lunteren, Erik Moyer, Michelle Gene expression of sternohyoid and diaphragm muscles in type 2 diabetic rats |
title | Gene expression of sternohyoid and diaphragm muscles in type 2 diabetic rats |
title_full | Gene expression of sternohyoid and diaphragm muscles in type 2 diabetic rats |
title_fullStr | Gene expression of sternohyoid and diaphragm muscles in type 2 diabetic rats |
title_full_unstemmed | Gene expression of sternohyoid and diaphragm muscles in type 2 diabetic rats |
title_short | Gene expression of sternohyoid and diaphragm muscles in type 2 diabetic rats |
title_sort | gene expression of sternohyoid and diaphragm muscles in type 2 diabetic rats |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3851765/ https://www.ncbi.nlm.nih.gov/pubmed/24199937 http://dx.doi.org/10.1186/1472-6823-13-43 |
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