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Inactivation of the novel avian influenza A (H7N9) virus under physical conditions or chemical agents treatment

BACKGROUND: In the spring of 2013, a novel avian-origin influenza A (H7N9) virus in Eastern China emerged causing human infections. Concerns that a new influenza pandemic could occur were raised. The potential effect of chemical agents and physical conditions on inactivation of the novel avian influ...

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Autores principales: Zou, Shumei, Guo, Junfeng, Gao, Rongbao, Dong, Libo, Zhou, Jianfang, Zhang, Ye, Dong, Jie, Bo, Hong, Qin, Kun, Shu, Yuelong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3852039/
https://www.ncbi.nlm.nih.gov/pubmed/24034697
http://dx.doi.org/10.1186/1743-422X-10-289
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author Zou, Shumei
Guo, Junfeng
Gao, Rongbao
Dong, Libo
Zhou, Jianfang
Zhang, Ye
Dong, Jie
Bo, Hong
Qin, Kun
Shu, Yuelong
author_facet Zou, Shumei
Guo, Junfeng
Gao, Rongbao
Dong, Libo
Zhou, Jianfang
Zhang, Ye
Dong, Jie
Bo, Hong
Qin, Kun
Shu, Yuelong
author_sort Zou, Shumei
collection PubMed
description BACKGROUND: In the spring of 2013, a novel avian-origin influenza A (H7N9) virus in Eastern China emerged causing human infections. Concerns that a new influenza pandemic could occur were raised. The potential effect of chemical agents and physical conditions on inactivation of the novel avian influenza H7N9 virus had not been assessed. METHODS: To determine the inactivation effectiveness of the novel avian influenza A (H7N9) virus under various physical conditions and chemical treatments, two H7N9 viruses A/Anhui/1/2013 and A/Shanghai/1/2013 were treated by varied temperatures, ultraviolet light, varied pHs and different disinfectants. The viruses with10(7.7) EID(50) were exposed to physical conditions (temperature, ultraviolet light and pH) or treated with commercial chemical agents (Sodium Hypochlorite, Virkon®-S, and Ethanol) respectively. After these treatments, the viruses were inoculated in SPF embryonated chicken eggs, the allantoic fluid was collected after 72–96 hours culture at 35°C and tested by haemagglutination assay. RESULTS: Both of the tested viruses could tolerate conditions under 56°C for 15 minutes or 60°C for 5 minutes, but their infectivity was completely lost under 56°C for 30 minutes, 65°C for 10 minutes, 70°C, 75°C and 100°C for 1 minute. It was also observed that the H7N9 viruses lost their infectivity totally after exposure of ultraviolet light irradiation for 30 minutes or longer time. Additionally, the viruses were completely inactivated at pH less than 2 for 0.5 hour or pH 3 for 24 hours, however, viruses remained infectious under pH treatment of 4–12 for 24 hours. The viruses were totally disinfected when treated with Sodium Hypochlorite, Virkon®-S and Ethanol at recommended concentrations after only 5 minutes. CONCLUSIONS: The novel avian influenza A (H7N9) virus can be inactivated under some physical conditions or with chemical treatments, but they present high tolerance to moderately acidic or higher alkali conditions. The results provided the essential information for public health intervention of novel H7N9 avian influenza outbreak.
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spelling pubmed-38520392013-12-06 Inactivation of the novel avian influenza A (H7N9) virus under physical conditions or chemical agents treatment Zou, Shumei Guo, Junfeng Gao, Rongbao Dong, Libo Zhou, Jianfang Zhang, Ye Dong, Jie Bo, Hong Qin, Kun Shu, Yuelong Virol J Research BACKGROUND: In the spring of 2013, a novel avian-origin influenza A (H7N9) virus in Eastern China emerged causing human infections. Concerns that a new influenza pandemic could occur were raised. The potential effect of chemical agents and physical conditions on inactivation of the novel avian influenza H7N9 virus had not been assessed. METHODS: To determine the inactivation effectiveness of the novel avian influenza A (H7N9) virus under various physical conditions and chemical treatments, two H7N9 viruses A/Anhui/1/2013 and A/Shanghai/1/2013 were treated by varied temperatures, ultraviolet light, varied pHs and different disinfectants. The viruses with10(7.7) EID(50) were exposed to physical conditions (temperature, ultraviolet light and pH) or treated with commercial chemical agents (Sodium Hypochlorite, Virkon®-S, and Ethanol) respectively. After these treatments, the viruses were inoculated in SPF embryonated chicken eggs, the allantoic fluid was collected after 72–96 hours culture at 35°C and tested by haemagglutination assay. RESULTS: Both of the tested viruses could tolerate conditions under 56°C for 15 minutes or 60°C for 5 minutes, but their infectivity was completely lost under 56°C for 30 minutes, 65°C for 10 minutes, 70°C, 75°C and 100°C for 1 minute. It was also observed that the H7N9 viruses lost their infectivity totally after exposure of ultraviolet light irradiation for 30 minutes or longer time. Additionally, the viruses were completely inactivated at pH less than 2 for 0.5 hour or pH 3 for 24 hours, however, viruses remained infectious under pH treatment of 4–12 for 24 hours. The viruses were totally disinfected when treated with Sodium Hypochlorite, Virkon®-S and Ethanol at recommended concentrations after only 5 minutes. CONCLUSIONS: The novel avian influenza A (H7N9) virus can be inactivated under some physical conditions or with chemical treatments, but they present high tolerance to moderately acidic or higher alkali conditions. The results provided the essential information for public health intervention of novel H7N9 avian influenza outbreak. BioMed Central 2013-09-15 /pmc/articles/PMC3852039/ /pubmed/24034697 http://dx.doi.org/10.1186/1743-422X-10-289 Text en Copyright © 2013 Zou et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Zou, Shumei
Guo, Junfeng
Gao, Rongbao
Dong, Libo
Zhou, Jianfang
Zhang, Ye
Dong, Jie
Bo, Hong
Qin, Kun
Shu, Yuelong
Inactivation of the novel avian influenza A (H7N9) virus under physical conditions or chemical agents treatment
title Inactivation of the novel avian influenza A (H7N9) virus under physical conditions or chemical agents treatment
title_full Inactivation of the novel avian influenza A (H7N9) virus under physical conditions or chemical agents treatment
title_fullStr Inactivation of the novel avian influenza A (H7N9) virus under physical conditions or chemical agents treatment
title_full_unstemmed Inactivation of the novel avian influenza A (H7N9) virus under physical conditions or chemical agents treatment
title_short Inactivation of the novel avian influenza A (H7N9) virus under physical conditions or chemical agents treatment
title_sort inactivation of the novel avian influenza a (h7n9) virus under physical conditions or chemical agents treatment
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3852039/
https://www.ncbi.nlm.nih.gov/pubmed/24034697
http://dx.doi.org/10.1186/1743-422X-10-289
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