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T Cells at the Site of Autoimmune Inflammation Show Increased Potential for Trogocytosis

CD4+ T cells acquire membrane fragments from antigen-presenting-cells via a process termed trogocytosis. Identifying which CD4+ T cells undergo trogocytosis in co-culture with Ag-loaded APC can enrich for antigen-reactive T cells without knowledge of their fine specificity or cytokine-production pro...

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Autores principales: Haastert, Bettina, Mellanby, Richard J., Anderton, Stephen M., O'Connor, Richard A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3852262/
https://www.ncbi.nlm.nih.gov/pubmed/24324692
http://dx.doi.org/10.1371/journal.pone.0081404
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author Haastert, Bettina
Mellanby, Richard J.
Anderton, Stephen M.
O'Connor, Richard A.
author_facet Haastert, Bettina
Mellanby, Richard J.
Anderton, Stephen M.
O'Connor, Richard A.
author_sort Haastert, Bettina
collection PubMed
description CD4+ T cells acquire membrane fragments from antigen-presenting-cells via a process termed trogocytosis. Identifying which CD4+ T cells undergo trogocytosis in co-culture with Ag-loaded APC can enrich for antigen-reactive T cells without knowledge of their fine specificity or cytokine-production profiles. We sought to assess the suitability of this method to identify disease relevant effector and regulatory T cells during autoimmune inflammation. Trogocytosis efficiently identified MBP-reactive T cells in vitro and ex-vivo following immunization. However, Foxp3+ regulatory T cells constitutively displayed a higher rate of trogocytosis than their Foxp3- counterparts which limits the potential of trogocytosis to identify antigen-reactive Treg cells. During inflammation a locally elevated rate of trogocytosis (seen in both effector and regulatory T cells isolated from the inflamed CNS) precludes the use of trogocytosis as a measure of antigenic reactivity among cells taken from inflammatory sites. Our results indicate trogocytosis detection can enrich for Ag-reactive conventional T cells in the periphery but is limited in its ability to identify Ag-reactive Treg or T effector cells at sites of inflammation. Increased trogocytosis potential at inflammatory sites also draws into the question the biological significance of this phenomenon during inflammation, in Treg mediated suppression and for the maintenance of tolerance in health and disease.
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spelling pubmed-38522622013-12-09 T Cells at the Site of Autoimmune Inflammation Show Increased Potential for Trogocytosis Haastert, Bettina Mellanby, Richard J. Anderton, Stephen M. O'Connor, Richard A. PLoS One Research Article CD4+ T cells acquire membrane fragments from antigen-presenting-cells via a process termed trogocytosis. Identifying which CD4+ T cells undergo trogocytosis in co-culture with Ag-loaded APC can enrich for antigen-reactive T cells without knowledge of their fine specificity or cytokine-production profiles. We sought to assess the suitability of this method to identify disease relevant effector and regulatory T cells during autoimmune inflammation. Trogocytosis efficiently identified MBP-reactive T cells in vitro and ex-vivo following immunization. However, Foxp3+ regulatory T cells constitutively displayed a higher rate of trogocytosis than their Foxp3- counterparts which limits the potential of trogocytosis to identify antigen-reactive Treg cells. During inflammation a locally elevated rate of trogocytosis (seen in both effector and regulatory T cells isolated from the inflamed CNS) precludes the use of trogocytosis as a measure of antigenic reactivity among cells taken from inflammatory sites. Our results indicate trogocytosis detection can enrich for Ag-reactive conventional T cells in the periphery but is limited in its ability to identify Ag-reactive Treg or T effector cells at sites of inflammation. Increased trogocytosis potential at inflammatory sites also draws into the question the biological significance of this phenomenon during inflammation, in Treg mediated suppression and for the maintenance of tolerance in health and disease. Public Library of Science 2013-12-04 /pmc/articles/PMC3852262/ /pubmed/24324692 http://dx.doi.org/10.1371/journal.pone.0081404 Text en © 2013 Haastert et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Haastert, Bettina
Mellanby, Richard J.
Anderton, Stephen M.
O'Connor, Richard A.
T Cells at the Site of Autoimmune Inflammation Show Increased Potential for Trogocytosis
title T Cells at the Site of Autoimmune Inflammation Show Increased Potential for Trogocytosis
title_full T Cells at the Site of Autoimmune Inflammation Show Increased Potential for Trogocytosis
title_fullStr T Cells at the Site of Autoimmune Inflammation Show Increased Potential for Trogocytosis
title_full_unstemmed T Cells at the Site of Autoimmune Inflammation Show Increased Potential for Trogocytosis
title_short T Cells at the Site of Autoimmune Inflammation Show Increased Potential for Trogocytosis
title_sort t cells at the site of autoimmune inflammation show increased potential for trogocytosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3852262/
https://www.ncbi.nlm.nih.gov/pubmed/24324692
http://dx.doi.org/10.1371/journal.pone.0081404
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