A new approach to 'megaprimer' polymerase chain reaction mutagenesis without an intermediate gel purification step

BACKGROUND: Site-directed mutagenesis is an efficient method to alter the structure and function of genes. Here we report a rapid and efficient megaprimer-based polymerase chain reaction (PCR) mutagenesis strategy that by-passes any intermediate purification of DNA between two rounds of PCR. RESULTS...

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Detalles Bibliográficos
Autores principales: Tyagi, Rajiv, Lai, Richard, Duggleby, Ronald G
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2004
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC385241/
https://www.ncbi.nlm.nih.gov/pubmed/15070414
http://dx.doi.org/10.1186/1472-6750-4-2

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC385241/
https://www.ncbi.nlm.nih.gov/pubmed/15070414
http://dx.doi.org/10.1186/1472-6750-4-2

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