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A new approach to 'megaprimer' polymerase chain reaction mutagenesis without an intermediate gel purification step

BACKGROUND: Site-directed mutagenesis is an efficient method to alter the structure and function of genes. Here we report a rapid and efficient megaprimer-based polymerase chain reaction (PCR) mutagenesis strategy that by-passes any intermediate purification of DNA between two rounds of PCR. RESULTS...

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Detalles Bibliográficos
Autores principales:	Tyagi, Rajiv, Lai, Richard, Duggleby, Ronald G
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2004
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC385241/ https://www.ncbi.nlm.nih.gov/pubmed/15070414 http://dx.doi.org/10.1186/1472-6750-4-2

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