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Transcription through enhancers suppresses their activity in Drosophila

BACKGROUND: Enhancer elements determine the level of target gene transcription in a tissue-specific manner, providing for individual patterns of gene expression in different cells. Knowledge of the mechanisms controlling enhancer action is crucial for understanding global regulation of transcription...

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Autores principales: Erokhin, Maksim, Davydova, Anna, Parshikov, Alexander, Studitsky, Vasily M, Georgiev, Pavel, Chetverina, Darya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3852481/
https://www.ncbi.nlm.nih.gov/pubmed/24279291
http://dx.doi.org/10.1186/1756-8935-6-31
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author Erokhin, Maksim
Davydova, Anna
Parshikov, Alexander
Studitsky, Vasily M
Georgiev, Pavel
Chetverina, Darya
author_facet Erokhin, Maksim
Davydova, Anna
Parshikov, Alexander
Studitsky, Vasily M
Georgiev, Pavel
Chetverina, Darya
author_sort Erokhin, Maksim
collection PubMed
description BACKGROUND: Enhancer elements determine the level of target gene transcription in a tissue-specific manner, providing for individual patterns of gene expression in different cells. Knowledge of the mechanisms controlling enhancer action is crucial for understanding global regulation of transcription. In particular, enhancers are often localized within transcribed regions of the genome. A number of experiments suggest that transcription can have both positive and negative effects on regulatory elements. In this study, we performed direct tests for the effect of transcription on enhancer activity. RESULTS: Using a transgenic reporter system, we investigated the relationship between the presence of pass-through transcription and the activity of Drosophila enhancers controlling the expression of the white and yellow genes. The results show that transcription from different promoters affects the activity of enhancers, counteracting their ability to activate the target genes. As expected, the presence of a transcriptional terminator between the inhibiting promoter and the affected enhancer strongly reduces the suppression. Moreover, transcription leads to dislodging of the Zeste protein that is responsible for the enhancer-dependent regulation of the white gene, suggesting a 'transcription interference’ mechanism for this regulation. CONCLUSIONS: Our findings suggest a role for pass-through transcription in negative regulation of enhancer activity.
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spelling pubmed-38524812013-12-06 Transcription through enhancers suppresses their activity in Drosophila Erokhin, Maksim Davydova, Anna Parshikov, Alexander Studitsky, Vasily M Georgiev, Pavel Chetverina, Darya Epigenetics Chromatin Research BACKGROUND: Enhancer elements determine the level of target gene transcription in a tissue-specific manner, providing for individual patterns of gene expression in different cells. Knowledge of the mechanisms controlling enhancer action is crucial for understanding global regulation of transcription. In particular, enhancers are often localized within transcribed regions of the genome. A number of experiments suggest that transcription can have both positive and negative effects on regulatory elements. In this study, we performed direct tests for the effect of transcription on enhancer activity. RESULTS: Using a transgenic reporter system, we investigated the relationship between the presence of pass-through transcription and the activity of Drosophila enhancers controlling the expression of the white and yellow genes. The results show that transcription from different promoters affects the activity of enhancers, counteracting their ability to activate the target genes. As expected, the presence of a transcriptional terminator between the inhibiting promoter and the affected enhancer strongly reduces the suppression. Moreover, transcription leads to dislodging of the Zeste protein that is responsible for the enhancer-dependent regulation of the white gene, suggesting a 'transcription interference’ mechanism for this regulation. CONCLUSIONS: Our findings suggest a role for pass-through transcription in negative regulation of enhancer activity. BioMed Central 2013-09-26 /pmc/articles/PMC3852481/ /pubmed/24279291 http://dx.doi.org/10.1186/1756-8935-6-31 Text en Copyright © 2013 Erokhin et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Erokhin, Maksim
Davydova, Anna
Parshikov, Alexander
Studitsky, Vasily M
Georgiev, Pavel
Chetverina, Darya
Transcription through enhancers suppresses their activity in Drosophila
title Transcription through enhancers suppresses their activity in Drosophila
title_full Transcription through enhancers suppresses their activity in Drosophila
title_fullStr Transcription through enhancers suppresses their activity in Drosophila
title_full_unstemmed Transcription through enhancers suppresses their activity in Drosophila
title_short Transcription through enhancers suppresses their activity in Drosophila
title_sort transcription through enhancers suppresses their activity in drosophila
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3852481/
https://www.ncbi.nlm.nih.gov/pubmed/24279291
http://dx.doi.org/10.1186/1756-8935-6-31
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