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Sorghum mutant RG displays antithetic leaf shoot lignin accumulation resulting in improved stem saccharification properties
BACKGROUND: Improving saccharification efficiency in bioenergy crop species remains an important challenge. Here, we report the characterization of a Sorghum (Sorghum bicolor L.) mutant, named REDforGREEN (RG), as a bioenergy feedstock. RESULTS: It was found that RG displayed increased accumulation...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3852544/ https://www.ncbi.nlm.nih.gov/pubmed/24103129 http://dx.doi.org/10.1186/1754-6834-6-146 |
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author | Petti, Carloalberto Harman-Ware, Anne E Tateno, Mizuki Kushwaha, Rekha Shearer, Andrew Downie, A Bruce Crocker, Mark DeBolt, Seth |
author_facet | Petti, Carloalberto Harman-Ware, Anne E Tateno, Mizuki Kushwaha, Rekha Shearer, Andrew Downie, A Bruce Crocker, Mark DeBolt, Seth |
author_sort | Petti, Carloalberto |
collection | PubMed |
description | BACKGROUND: Improving saccharification efficiency in bioenergy crop species remains an important challenge. Here, we report the characterization of a Sorghum (Sorghum bicolor L.) mutant, named REDforGREEN (RG), as a bioenergy feedstock. RESULTS: It was found that RG displayed increased accumulation of lignin in leaves and depletion in the stems, antithetic to the trend observed in wild type. Consistent with these measurements, the RG leaf tissue displayed reduced saccharification efficiency whereas the stem saccharification efficiency increased relative to wild type. Reduced lignin was linked to improved saccharification in RG stems, but a chemical shift to greater S:G ratios in RG stem lignin was also observed. Similarities in cellulose content and structure by XRD-analysis support the correlation between increased saccharification properties and reduced lignin instead of changes in the cellulose composition and/or structure. CONCLUSION: Antithetic lignin accumulation was observed in the RG mutant leaf-and stem-tissue, which resulted in greater saccharification efficiency in the RG stem and differential thermochemical product yield in high lignin leaves. Thus, the red leaf coloration of the RG mutant represents a potential marker for improved conversion of stem cellulose to fermentable sugars in the C4 grass Sorghum. |
format | Online Article Text |
id | pubmed-3852544 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-38525442013-12-06 Sorghum mutant RG displays antithetic leaf shoot lignin accumulation resulting in improved stem saccharification properties Petti, Carloalberto Harman-Ware, Anne E Tateno, Mizuki Kushwaha, Rekha Shearer, Andrew Downie, A Bruce Crocker, Mark DeBolt, Seth Biotechnol Biofuels Research BACKGROUND: Improving saccharification efficiency in bioenergy crop species remains an important challenge. Here, we report the characterization of a Sorghum (Sorghum bicolor L.) mutant, named REDforGREEN (RG), as a bioenergy feedstock. RESULTS: It was found that RG displayed increased accumulation of lignin in leaves and depletion in the stems, antithetic to the trend observed in wild type. Consistent with these measurements, the RG leaf tissue displayed reduced saccharification efficiency whereas the stem saccharification efficiency increased relative to wild type. Reduced lignin was linked to improved saccharification in RG stems, but a chemical shift to greater S:G ratios in RG stem lignin was also observed. Similarities in cellulose content and structure by XRD-analysis support the correlation between increased saccharification properties and reduced lignin instead of changes in the cellulose composition and/or structure. CONCLUSION: Antithetic lignin accumulation was observed in the RG mutant leaf-and stem-tissue, which resulted in greater saccharification efficiency in the RG stem and differential thermochemical product yield in high lignin leaves. Thus, the red leaf coloration of the RG mutant represents a potential marker for improved conversion of stem cellulose to fermentable sugars in the C4 grass Sorghum. BioMed Central 2013-10-09 /pmc/articles/PMC3852544/ /pubmed/24103129 http://dx.doi.org/10.1186/1754-6834-6-146 Text en Copyright © 2013 Petti et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Petti, Carloalberto Harman-Ware, Anne E Tateno, Mizuki Kushwaha, Rekha Shearer, Andrew Downie, A Bruce Crocker, Mark DeBolt, Seth Sorghum mutant RG displays antithetic leaf shoot lignin accumulation resulting in improved stem saccharification properties |
title | Sorghum mutant RG displays antithetic leaf shoot lignin accumulation resulting in improved stem saccharification properties |
title_full | Sorghum mutant RG displays antithetic leaf shoot lignin accumulation resulting in improved stem saccharification properties |
title_fullStr | Sorghum mutant RG displays antithetic leaf shoot lignin accumulation resulting in improved stem saccharification properties |
title_full_unstemmed | Sorghum mutant RG displays antithetic leaf shoot lignin accumulation resulting in improved stem saccharification properties |
title_short | Sorghum mutant RG displays antithetic leaf shoot lignin accumulation resulting in improved stem saccharification properties |
title_sort | sorghum mutant rg displays antithetic leaf shoot lignin accumulation resulting in improved stem saccharification properties |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3852544/ https://www.ncbi.nlm.nih.gov/pubmed/24103129 http://dx.doi.org/10.1186/1754-6834-6-146 |
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