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Insights into the mechanism of X-ray-induced disulfide-bond cleavage in lysozyme crystals based on EPR, optical absorption and X-ray diffraction studies
Electron paramagnetic resonance (EPR) and online UV–visible absorption microspectrophotometry with X-ray crystallography have been used in a complementary manner to follow X-ray-induced disulfide-bond cleavage. Online UV–visible spectroscopy showed that upon X-irradiation, disulfide radicalization a...
Autores principales: | Sutton, Kristin A., Black, Paul J., Mercer, Kermit R., Garman, Elspeth F., Owen, Robin L., Snell, Edward H., Bernhard, William A. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3852651/ https://www.ncbi.nlm.nih.gov/pubmed/24311579 http://dx.doi.org/10.1107/S0907444913022117 |
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